Open Access

NCAPD2 augments the tumorigenesis and progression of human liver cancer via the PI3K‑Akt‑mTOR signaling pathway

  • Authors:
    • Jiang-Xue Gu
    • Ke Huang
    • Wei-Lin Zhao
    • Xiao-Ming Zheng
    • Yu-Qin Wu
    • Shi-Rong Yan
    • Yu-Gang Huang
    • Pei Hu
  • View Affiliations

  • Published online on: July 31, 2024     https://doi.org/10.3892/ijmm.2024.5408
  • Article Number: 84
  • Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Non‑SMC condensin I complex subunit D2 (NCAPD2) is a newly identified oncogene; however, the specific biological function and molecular mechanism of NCAPD2 in liver cancer progression remain unknown. In the present study, the aberrant expression of NCAPD2 in liver cancer was investigated using public tumor databases, including TNMplot, The Cancer Genome Atlas and the International Cancer Genome Consortium based on bioinformatics analyses, and it was validated using a clinical cohort. It was revealed that NCAPD2 was significantly upregulated in liver cancer tissues compared with in control liver tissues, and NCAPD2 served as an independent prognostic factor and predicted poor prognosis in liver cancer. In addition, the expression of NCAPD2 was positively correlated with the percentage of Ki67+ cells. Finally, single‑cell sequencing data, gene‑set enrichment analyses and in vitro investigations, including cell proliferation assay, Transwell assay, wound healing assay, cell cycle experiments, cell apoptosis assay and western blotting, were carried out in human liver cancer cell lines to assess the biological mechanisms of NCAPD2 in patients with liver cancer. The results revealed that the upregulation of NCAPD2 enhanced tumor cell proliferation, invasion and cell cycle progression at the G2/M‑phase transition, and inhibited apoptosis in liver cancer cells. Furthermore, NCAPD2 overexpression was closely associated with the phosphatidylinositol 3‑kinase (PI3K)‑Akt‑mammalian target of rapamycin (mTOR)/c‑Myc signaling pathway and epithelial‑mesenchymal transition (EMT) progression in HepG2 and Huh7 cells. In addition, upregulated NCAPD2 was shown to have adverse effects on overall survival and disease‑specific survival in liver cancer. In conclusion, the overexpression of NCAPD2 was shown to lead to cell cycle progression at the G2/M‑phase transition, activation of the PI3K‑Akt‑mTOR/c‑Myc signaling pathway and EMT progression in human liver cancer cells.
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October-2024
Volume 54 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Gu J, Huang K, Zhao W, Zheng X, Wu Y, Yan S, Huang Y and Hu P: NCAPD2 augments the tumorigenesis and progression of human liver cancer via the PI3K‑Akt‑mTOR signaling pathway. Int J Mol Med 54: 84, 2024.
APA
Gu, J., Huang, K., Zhao, W., Zheng, X., Wu, Y., Yan, S. ... Hu, P. (2024). NCAPD2 augments the tumorigenesis and progression of human liver cancer via the PI3K‑Akt‑mTOR signaling pathway. International Journal of Molecular Medicine, 54, 84. https://doi.org/10.3892/ijmm.2024.5408
MLA
Gu, J., Huang, K., Zhao, W., Zheng, X., Wu, Y., Yan, S., Huang, Y., Hu, P."NCAPD2 augments the tumorigenesis and progression of human liver cancer via the PI3K‑Akt‑mTOR signaling pathway". International Journal of Molecular Medicine 54.4 (2024): 84.
Chicago
Gu, J., Huang, K., Zhao, W., Zheng, X., Wu, Y., Yan, S., Huang, Y., Hu, P."NCAPD2 augments the tumorigenesis and progression of human liver cancer via the PI3K‑Akt‑mTOR signaling pathway". International Journal of Molecular Medicine 54, no. 4 (2024): 84. https://doi.org/10.3892/ijmm.2024.5408