Transformation of rodent fibroblasts by ICP4, the major transactivating protein of herpes simplex virus type 1

  • Authors:
    • A Descalzo
    • C Weiand
    • L Dietze
    • B Matz
    • G Bauer
  • View Affiliations

  • Published online on: April 1, 1997     https://doi.org/10.3892/ijo.10.4.765
  • Pages: 765-773
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Abstract

Rat fibroblasts were transfected with a plasmid containing the IE3 gene derived from the temperature sensitive HSV-1 mutant tsK. Three of four clones expressing biologically active, temperature-sensitive ICP4, formed a substantial number of colonies in soft agar at the permissive temperature. During the first passages of cells, the transformed state of the major proportion of transformed cells was dependent on the continuous activity of ICP4. In a smaller and distinct subpopulation of transformed cells, as well as after longer subcloning of cells, ICP4 was no longer required for the maintenance of the transformed state, pointing to the induction of stable genomic changes by ICP4. Our data show that ICP4 of HSV-1 is involved in transformation of fibroblasts. Transformed cells are, however, subject to intracellular and intercellular control mechanisms.

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April 1997
Volume 10 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Descalzo A, Weiand C, Dietze L, Matz B and Bauer G: Transformation of rodent fibroblasts by ICP4, the major transactivating protein of herpes simplex virus type 1. Int J Oncol 10: 765-773, 1997.
APA
Descalzo, A., Weiand, C., Dietze, L., Matz, B., & Bauer, G. (1997). Transformation of rodent fibroblasts by ICP4, the major transactivating protein of herpes simplex virus type 1. International Journal of Oncology, 10, 765-773. https://doi.org/10.3892/ijo.10.4.765
MLA
Descalzo, A., Weiand, C., Dietze, L., Matz, B., Bauer, G."Transformation of rodent fibroblasts by ICP4, the major transactivating protein of herpes simplex virus type 1". International Journal of Oncology 10.4 (1997): 765-773.
Chicago
Descalzo, A., Weiand, C., Dietze, L., Matz, B., Bauer, G."Transformation of rodent fibroblasts by ICP4, the major transactivating protein of herpes simplex virus type 1". International Journal of Oncology 10, no. 4 (1997): 765-773. https://doi.org/10.3892/ijo.10.4.765