The anticancer effects of Saccharina japonica on 267B1/K-ras human prostate cancer cells

Jo,Mi  Jeong; Kim,Hyung  Rak; Kim,Gun  Do

doi:10.3892/ijo.2012.1612

The anticancer effects of Saccharina japonica on 267B1/K-ras human prostate cancer cells

Authors:
- Mi Jeong Jo
- Hyung Rak Kim
- Gun Do Kim
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Affiliations: Department of Microbiology, College of Natural Sciences, Pukyung National University, Busan 608-737, Republic of Korea, Department of Food Science and Nutrition, College of Fisheries Sciences, Pukyung National University, Busan 608-737, Republic of Korea
Published online on: August 30, 2012 https://doi.org/10.3892/ijo.2012.1612
Pages: 1789-1797

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Abstract

Saccharina japonica (S. japonica), a brown macro-alga, has been used as a traditional medicine in Korea for thousands of years. In this study, the potential anticancer effects of S. japonica were evaluated on 267B1/K-ras human prostate cancer cells. The exposure of cells to the extract induced inhibition of cell growth by increasing the number of apoptotic cells with cell shrinkage and inhibition of cell cycle progression. The effects of the extract on the cells were assessed by studying the cleavage of caspases and the target proteins of caspases. The increased expression of various cleaved caspases and changed expression of other proteins related in the apoptosis pathway were observed. 4'-6-Diamidino-2-phenylindole (DAPI) and immunofluorescence staining showed the cells undergoing apoptosis. Apoptosis induced changes in the expression of proteins involved in a variety of signaling pathways such as endocellular reticulum (ER) stress, death receptor and mammalian target of rapamycin (mTOR)-FoxO-mediated pathways. The data suggest that the extract (n-hexane sub-fraction) of S. japonica, induces apoptosis and cell cycle arrest in 267B1/K-ras human prostate cancer cells, and has potential as a complementary agent for cancer prevention.

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1.	Parda DS, Thraves PJ, Kuettel MR, et al: Neoplastic transformation of a human prostate epithelial cell line by the v-Ki-ras oncogene. Prostate. 23:91–98. 1993. View Article : Google Scholar : PubMed/NCBI
2.	Wynder EL, Rose DP and Cohen LA: Nutrition and prostate cancer: a proposal for dietary intervention. Nutr Cancer. 22:1–10. 1994. View Article : Google Scholar : PubMed/NCBI
3.	Giovannucci E: Epidemiologic characteristics of prostate cancer. Cancer. 75:1766–1777. 1995. View Article : Google Scholar
4.	Stan SD, Kar S, Stoner GD and Singh SV: Bioactive food components and cancer risk reduction. J Cell Biochem. 104:339–356. 2008. View Article : Google Scholar : PubMed/NCBI
5.	Booth E: Trace elements and seaweeds. Proceeding of the 4th International seaweed symposium. De Virville AD and Feldmann J: MacMillan; London: pp. 385–393. 1964
6.	Yan X, Chuda Y, Suzuki M and Nagata T: Fucoxanthin as the major antioxidant in Hijikia fusiformis, a common edible seaweed. Biosci Biotechnol Biochem. 63:605–607. 1999. View Article : Google Scholar : PubMed/NCBI
7.	Munro MHG, Blunt JW, Dumdei EJ, et al: The discovery and development of marine compounds with pharmaceutical potential. J Biotechnol. 70:15–25. 1999. View Article : Google Scholar : PubMed/NCBI
8.	Rice-Evans C, Miller N and Paganga G: Antioxidant properties of phenolic compounds. Trends Plant Sci. 2:152–159. 1997. View Article : Google Scholar
9.	Yuan YV and Walsh NA: Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds. Food Chem Toxicol. 44:1144–1150. 2006. View Article : Google Scholar : PubMed/NCBI
10.	Lee NY, Ermakova SP, Zvyagintseva TN, Kang KW, Dong Z and Choi HS: Inhibitory effects of fucoidan on activation of epidermal growth factor receptor and cell transformation in JB6 Cl41 cells. Food Chem Toxicol. 46:1793–1800. 2008. View Article : Google Scholar : PubMed/NCBI
11.	Lieberthal W, Koh JS and Levine JS: Necrosis and apoptosis in acute renal failure. Semin Nephrol. 18:505–518. 1998.PubMed/NCBI
12.	Zimmermann KC, Bonzon C and Green DR: The machinery of programmed cell death. Pharmacol Ther. 92:57–70. 2001. View Article : Google Scholar : PubMed/NCBI
13.	Cryns VL, Bergeron L, Zhu H, Li H and Yuan J: Specific cleavage of α-fodrin during Fas-and tumor necrosis factor-induced apoptosis is mediated by an interleukin-1β-converting enzyme/Ced-3 protease distinct from the poly(ADP-ribose) polymerase protease. J Biol Chem. 271:31277–31282. 1996.
14.	Kothakota S, Azuma T, Reinhard C, et al: Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis. Science. 278:294–298. 1997. View Article : Google Scholar : PubMed/NCBI
15.	Lazebnik Y, Kaufmann S, Desnoyers S, Poirier G and Earnshaw W: Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature. 371:346–347. 1994. View Article : Google Scholar : PubMed/NCBI
16.	Lazebnik YA, Takahashi A, Moir RD, et al: Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution. Proc Natl Acad Sci USA. 92:9042–9046. 1995. View Article : Google Scholar : PubMed/NCBI
17.	Mashima T, Naito M, Fujita N, Noguchi K and Tsuruo T: Identification of actin as a substrate of ICE and an ICE-like protease and involvement of an ICE-like protease but not ICE in VP-16-induced U937 apoptosis. Biochem Biophys Res Commun. 217:1185–1192. 1995. View Article : Google Scholar : PubMed/NCBI
18.	Sakahira H, Enari M and Nagata S: Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature. 391:96–99. 1998. View Article : Google Scholar : PubMed/NCBI
19.	Sherr CJ: Gl phase progression: cycling on cue. Cell. 79:551–555. 1994. View Article : Google Scholar : PubMed/NCBI
20.	Xiong Y, Hannon G, Zhang H, Casso D, Kobayashi R and Beach D: p21 is a universal inhibitor of cyclin kinases. Nature. 366:701–704. 1993. View Article : Google Scholar : PubMed/NCBI
21.	Kwon O, Kim KA, Kim SO, et al: NF-κB inhibition increases chemosensitivity to trichostatin A-induced cell death of Ki-Ras-transformed human prostate epithelial cells. Carcinogenesis. 27:2258–2268. 2006.
22.	Kuo PL, Hsu YL, Cho CY, Ng LT, Kuo YH and Lin CC: Apoptotic effects of Antrodia cinnamomea1 fruiting bodies extract are mediated through calcium and calpain-dependent pathways in Hep 3B cells. Food Chem Toxicol. 44:1316–1326. 2006. View Article : Google Scholar : PubMed/NCBI
23.	Rasheva VI and Domingos PM: Cellular responses to endoplasmic reticulum stress and apoptosis. Apoptosis. 14:996–1007. 2009. View Article : Google Scholar : PubMed/NCBI
24.	Muzio M, Chinnaiyan AM, Kischkel FC, et al: FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex. Cell. 85:817–827. 1996. View Article : Google Scholar : PubMed/NCBI
25.	Sabatini DM: mTOR and cancer: insights into a complex relationship. Nat Rev Cancer. 6:729–734. 2006. View Article : Google Scholar : PubMed/NCBI
26.	Park SJ, Sohn HY, Yoon J and Park SI: Down-regulation of FoxO-dependent c-FLIP expression mediates TRAIL-induced apoptosis in activated hepatic stellate cells. Cell Signal. 21:1495–1503. 2009. View Article : Google Scholar : PubMed/NCBI
27.	Oberhammer FA, Hochegger K, Fröschl G, Tiefenbacher R and Pavelka M: Chromatin condensation during apoptosis is accompanied by degradation of lamin A+B, without enhanced activation of cdc2 kinase. J Cell Biol. 126:827–837. 1994.
28.	Sørensen CS, Syljuåsen RG, Falck J, et al: Chk1 regulates the S phase checkpoint by coupling the physiological turnover and ionizing radiation-induced accelerated proteolysis of Cdc25A. Cancer Cell. 3:247–258. 2003.PubMed/NCBI
29.	Gartel AL and Tyner AL: The role of the cyclin-dependent kinase inhibitor p21 in apoptosis 1 supported in part by NIH grant R01 DK56283 (to ALT) for the p21 research and Campus Research Board and Illinois Department of Public Health Penny Severns Breast and Cervical Cancer grants (to ALG). 1. Mol Cancer Ther. 1:6392002.