Tetraspanin 1 promotes invasiveness of cervical cancer cells

Hölters,Sebastian; Anacker,Jelena; Jansen,Lars; Beer-Grondke,Katrin; Dürst,Matthias; Rubio,Ignacio

doi:10.3892/ijo.2013.1980

Tetraspanin 1 promotes invasiveness of cervical cancer cells

Authors:
- Sebastian Hölters
- Jelena Anacker
- Lars Jansen
- Katrin Beer-Grondke
- Matthias Dürst
- Ignacio Rubio
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Affiliations: Department of Gynecology and Obstretrics, Jena University Hospital, Jena, Germany, Department of Obstretrics and Gynecology, University of Würzburg, Würzburg, Germany, Center for Molecular Biomedicine, Institute of Molecular Cell Biology, Jena University Hospital, Jena, Germany
Published online on: June 10, 2013 https://doi.org/10.3892/ijo.2013.1980
Pages: 503-512

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Abstract

Tetraspanins are a heterogeneous group of 4-transmembrane proteins that segregate into so-called tetraspanin-enriched microdomains (TEMs) along with other cell surface proteins such as integrins. TEMs of various types are reportedly involved in the regulation of cell growth, migration and invasion of several tumour cell types, both as suppressors or supporting structures. Tetraspanin 1 (Tspan1, NET-1), a member of the transmembrane 4 superfamily (TM4SF) of tetraspanins, is overexpressed in high-grade cervical intraepithelial neoplasia (CIN) and terminal carcinomas but its precise function in the context of carcinoma of the cervix uteri is not known. Here, we present a comprehensive investigation of the role of tetraspanin 1 in the cervical cancer cell lines SiHa and HeLa. We document that tetraspanin 1 increases the invasive potential of cervical cancer cells, whereas proliferation, growth in soft agar and adhesion are largely unaffected. In line with the latter findings, our data exclude the participation of testraspanin in integrin-mediated activation of focal adhesion kinase (FAK), paxillin and phosphoinositide-3-kinase (PI3K) and in EGFR-dependent signalling to the Ras/Erk pathway. In conclusion, our data argue against a role for tetraspanin 1 as a genuine mediator of cell surface receptor signalling but rather document a role for tetraspanin 1 in the control of cervical cancer cell motility and invasion.

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