Isolation of mesenchymal stem-like cells in meningioma specimens

Lim,Hyo-Yeol; Kim,Kyung Min; Kim,Bo Kyung; Shim,Jin-Kyoung; Lee,Ji-Hyun; Huh,Yong-Min; Kim,Se-Hoon; Kim,Eui-Hyun; Park,Eun-Kyung; Shim,Kyu-Won; Chang,Jong Hee; Kim,Dong-Seok; Kim,Sun   Ho; Hong,Yong-Kil; Lee,Su-Jae; Kang,Seok-Gu

doi:10.3892/ijo.2013.2053

Isolation of mesenchymal stem-like cells in meningioma specimens

Authors:
- Hyo-Yeol Lim
- Kyung Min Kim
- Bo Kyung Kim
- Jin-Kyoung Shim
- Ji-Hyun Lee
- Yong-Min Huh
- Se-Hoon Kim
- Eui-Hyun Kim
- Eun-Kyung Park
- Kyu-Won Shim
- Jong Hee Chang
- Dong-Seok Kim
- Sun Ho Kim
- Yong-Kil Hong
- Su-Jae Lee
- Seok-Gu Kang
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Affiliations: Department of Neurosurgery, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea, Department of Medical Science, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea, Department of Radiology, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea, Department of Pathology, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea, Department of Neurosurgery, Seoul St. Mary's Hospital, The Catholic University of Korea College of Medicine, Seoul, Republic of Korea, Department of Chemistry, Research Institute for Natural Sciences, Hanyang University, Seoul, Republic of Korea
Published online on: August 6, 2013 https://doi.org/10.3892/ijo.2013.2053
Pages: 1260-1268

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Abstract

Cells resembling bone marrow mesenchymal stem cells (BM-MSCs) have been isolated from glioma specimens; however, little is known about the existence of mesenchymal stem-like cells (MSLCs) in meningioma. Here, we hypothesized that cells similar to BM-MSCs exist in meningioma specimens and sought to investigate whether these putative meningioma stroma MSLCs (MS-MSLCs) could be isolated. To this end, we cultured fresh meningioma specimens using the same protocols as used previously to isolate BM-MSC. Cultured cells were analyzed for surface markers associated with BM-MSCs by fluorescence-activated cell sorting (FACS) and candidate cells were exposed to mesenchymal differentiation conditions. Possible locations of MS-MSLCs were determined by immunohistochemical analysis of sections of meningioma specimens. Spindle-shaped and, adherent cells similar to BM-MSCs were isolated in 2 of 20 meningioma specimens. FACS analysis showed that the surface markers of MS-MSLCs were similar to those of BM-MSCs and the chosen cells demonstrated an ability to differentiate into osteogenic, adipogenic and chondrogenic cells. The tumorigenicity of MS-MSLCs was tested by injection of these cells into the brain of athymic nude mice; no tumors were subsequently discovered. Immunohistochemical analyses indicated that CD105+ cells were closely associated with endothelial cells and pericytes in meningioma specimens. Our results established for the first time that cells similar to BM-MSCs exist in meningioma specimens. These cells, termed MS-MSLCs, could be one component of the meningioma cellular microenvironment.

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