Oleifolioside B-mediated autophagy promotes apoptosis in A549 human non-small cell lung cancer cells

Jin,Cheng-Yun; Yu,Hai  Yang; Park,Cheol; Han,Min   Ho; Hong,Su  Hyun; Kim,Kyoung-Sook; Lee,Young-Choon; Chang,Young-Chae; Cheong,Jaehun; Moon,Sung-Kwon; Kim,Gi-Young; Moon,Hyung-In; Kim,Wun-Jae; Lee,Jai-Heon; Choi,Yung  Hyun

doi:10.3892/ijo.2013.2143

Oleifolioside B-mediated autophagy promotes apoptosis in A549 human non-small cell lung cancer cells

Authors:
- Cheng-Yun Jin
- Hai Yang Yu
- Cheol Park
- Min Ho Han
- Su Hyun Hong
- Kyoung-Sook Kim
- Young-Choon Lee
- Young-Chae Chang
- Jaehun Cheong
- Sung-Kwon Moon
- Gi-Young Kim
- Hyung-In Moon
- Wun-Jae Kim
- Jai-Heon Lee
- Yung Hyun Choi
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Affiliations: School of Pharmaceutical Science, Zhengzhou University, Henan 450001, P.R. China., College of Natural Resources and Life Science, BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714, Republic of Korea, Department of Molecular Biology, College of Natural Sciences, Dongeui University, Busan 614-714, Republic of Korea, Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan 614-052, Republic of Korea, Research Institute of Biomedical Engineering and Department of Medicine, Catholic University of Daegu School of Medicine, Daegu 705-718, Republic of Korea, Department of Molecular Biology, College of Natural Sciences, Busan National University, Busan 609-735, Republic of Korea, School of Food Science and Technology, Chung-Ang University, Ansung 456-756, Republic of Korea, Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea, Department of Urology, Chungbuk National University College of Medicine, Cheongju 361-763, Republic of Korea
Published online on: October 17, 2013 https://doi.org/10.3892/ijo.2013.2143
Pages: 1943-1950

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Abstract

The biochemical mechanisms of cell death by oleifolioside B (OB), a cycloartane-type triterpene glycoside isolated from Dendropanax morbifera Leveille, were investigated in A549 human lung carcinoma cells. Our data indicated that exposure to OB led to caspase activation and typical features of apoptosis; however, apoptotic cell death was not prevented by z-VAD-fmk, a pan-caspase inhibitor, demonstrating that OB-induced apoptosis was independent of caspase activation. Subsequently, we found that OB increased autophagy, as indicated by an increase in monodansylcadaverine fluorescent dye-labeled autophagosome formation and in the levels of the autophagic form of microtubule-associated protein 1 light chain 3 and Atg3, an autophagy-specific gene, which is associated with inhibiting phospho-nuclear factor erythroid 2-related factor 2 (Nrf2) expression. However, pretreatment with bafilomycin A1, an autophagy inhibitor, attenuated OB-induced apoptosis and dephosphorylation of Nrf2. The data suggest that OB-induced autophagy functions as a death mechanism in A549 cells and OB has potential as a novel anticancer agent capable of targeting apoptotic and autophagic cell death and the Nrf2 signaling pathway.

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