Molecular profiling of chordoma

Scheil-Bertram,Stefanie; Kappler,Roland; von Baer,Alexandra; Hartwig,Erich; Sarkar,Michael; Serra,Massimo; Brüderlein,Silke; Westhoff,Bettina; Melzner,Ingo; Bassaly,Birgit; Herms,Jochen; Hugo,Heinz-Hermann; Schulte,Michael; Möller,Peter

doi:10.3892/ijo.2014.2268

Molecular profiling of chordoma

Authors:
- Stefanie Scheil-Bertram
- Roland Kappler
- Alexandra von Baer
- Erich Hartwig
- Michael Sarkar
- Massimo Serra
- Silke Brüderlein
- Bettina Westhoff
- Ingo Melzner
- Birgit Bassaly
- Jochen Herms
- Heinz-Hermann Hugo
- Michael Schulte
- Peter Möller
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Affiliations: Institute of Pathology, University Hospitals of Ulm, Germany, Department of Pediatric Surgery, Dr. von Hauner Children's Hospital, Ludwig-Maximilian University of Munich, Munich, Germany, Department of Orthopedic Trauma, Hand and Reconstructive Surgery, University Hospitals of Ulm, Germany, Department of Trauma, Hand and Reconstructive Surgery, Ev. Diakonissenanstalt, Karlsruhe, Germany, Department of Trauma and Reconstructive Surgery, Karl-Olga-Krankenhaus, Stuttgart, Germany, Laboratory of Experimental Oncology, Orthopedic Rizzoli Institute, Bologna, Italy, Department of Orthopedics, University of Düsseldorf, Germany, Institute of Pathology, University of Giessen, Germany, Department of Translational Brain Research, DZNE (German Center for Neurodegenerative Diseases) and Ludwig-Maximilian University of Munich, Munich, Germany, Department of Neurosurgery, University of Kiel, Kiel, Germany, Department of Trauma and Orthopedic Surgery, Diakoniekrankenhaus, Rotenburg (Wümme), Germany
Published online on: January 21, 2014 https://doi.org/10.3892/ijo.2014.2268
Pages: 1041-1055
Copyright: © Scheil-Bertram et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The molecular basis of chordoma is still poorly understood, particularly with respect to differentially expressed genes involved in the primary origin of chordoma. In this study, therefore, we compared the transcriptional expression profile of one sacral chordoma recurrence, two chordoma cell lines (U-CH1 and U-CH2) and one chondrosarcoma cell line (U-CS2) with vertebral disc using a high-density oligonucleotide array. The expression of 65 genes whose mRNA levels differed significantly (p<0.001; ≥6-fold change) between chordoma and control (vertebral disc) was identified. Genes with increased expression in chordoma compared to control and chondrosarcoma were most frequently located on chromosomes 2 (11%), 5 (8%), 1 and 7 (each 6%), whereas interphase cytogenetics of 33 chordomas demonstrated gains of chromosomal material most prevalent on 7q (42%), 12q (21%), 17q (21%), 20q (27%) and 22q (21%). The microarray data were confirmed for selected genes by quantitative polymerase chain reaction analysis. As in other studies, we showed the expression of brachyury. We demonstrate the expression of new potential candidates for chordoma tumorigenesis, such as CD24, ECRG4, RARRES2, IGFBP2, RAP1, HAI2, RAB38, osteopontin, GalNAc-T3, VAMP8 and others. Thus, we identified and validated a set of interesting candidate genes whose differential expression likely plays a role in chordoma.

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