Downregulation of Msi1 suppresses the growth of human colon cancer by targeting p21cip1

Gao,Chao; Han,Chun; Yu,Qiyao; Guan,Yue; Li,Na; Zhou,Jingjing; Tian,Yanming; Zhang,Yi

doi:10.3892/ijo.2014.2749

Downregulation of Msi1 suppresses the growth of human colon cancer by targeting p21cip1

Authors:
- Chao Gao
- Chun Han
- Qiyao Yu
- Yue Guan
- Na Li
- Jingjing Zhou
- Yanming Tian
- Yi Zhang
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Affiliations: Department of Radiation Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Nephrology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Physiology, Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
Published online on: November 13, 2014 https://doi.org/10.3892/ijo.2014.2749
Pages: 732-740

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Abstract

Musashi1 (Msi1), a member of the RNA-binding protein (RBP) family, is highly expressed in neural progenitor or stem cells for the maintenance of stemness as well as in various cancers. Emerging studies have demonstrated that it regulates cell processes by translational activation or suppresses specifically bound mRNA. In the present study, we initially reported remarkably increased expression of Msi1 in colon cancer tissues compared with adjacent non-tumor tissues. Knockdown of Msi1 significantly suppressed the proliferation, colony formation, tumorsphere formation and the progression of implanted colon cancers, and induced cell cycle attest at G0/G1 phase, along with the upregulated expression of p21cip1. Reporter assays using a chimeric mRNA that combined luciferase and the 3'-UTR of p21cip1 revealed that Msi1 decreased the reporter activity through the specific motif. Thus, the current results suggested that downregulation of Msi1 could inhibit the growth of colon cancers and Msi1 may be a promising therapeutic target molecule for human colon cancers.

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