Gallic acid exerts a protective or an anti-proliferative effect on glioma T98G cells via dose-dependent epigenetic regulation mediated by miRNAs

Paolini,Alessandro; Curti,Valeria; Pasi,Francesca; Mazzini,Giuliano; Nano,Rosanna; Capelli,Enrica

doi:10.3892/ijo.2015.2864

Gallic acid exerts a protective or an anti-proliferative effect on glioma T98G cells via dose-dependent epigenetic regulation mediated by miRNAs

Authors:
- Alessandro Paolini
- Valeria Curti
- Francesca Pasi
- Giuliano Mazzini
- Rosanna Nano
- Enrica Capelli
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Affiliations: Department of Biology and Biotechnology, Laboratory of Neuro Radio Experimental Biology, ‘Lazzaro Spallanzani’, University of Pavia, 27100 Pavia, Italy, Department of Earth and Environmental Sciences, Laboratory of Immunology and Genetic Analysis, University of Pavia, 27100 Pavia, Italy, IGM-CNR Histochemistry and Cytometry Section, Department of Animal Biology, University of Pavia, 27100 Pavia, Italy
Published online on: February 2, 2015 https://doi.org/10.3892/ijo.2015.2864
Pages: 1491-1497
Copyright: © Paolini et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Glioblastoma multiforme (GBM) is the most malignant primary brain tumor in adulthood, characterized by very high recurrence. Following the limited results for conventional therapies, novel therapeutic agents are under investigation. Among the putative new molecules, gallic acid (GA) represents a promising new anticancer drug. The anticancer effect of this drug has been based on its antioxidant effects. The aim of the present study was to investigate the toxic effects of GA on the T98G human glioblastoma cell line and its capacity to modulate the expression of microRNAs targeting the genes involved in tumor growth and invasion. Cytotoxicity, clonogenic ability and cell migration after GA treatment were tested. Moreover, the expression of miRNAs that target genes for antioxidant mitochondrial enzymes (mir-17-3p), p-21 protein (mir-21-5p) and ATM (mir-421-5p) was determined by qRT-PCR. The results confirmed in the T98G cells the anti-proliferative effect of GA reported for other glioma cell lines and showed that the miRNA expression changes depending on GA concentrations. Different GA concentrations can determine a protective or a toxic effect on tumor cells. Thus, the key for GA to induce a specific anticancer action is to use an optimal concentration that avoids these twin effects.

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