A novel curcuminoid exhibits enhanced antitumor activity in nasopharyngeal carcinoma

Pan,Yunbao; Liu,Guohong; Xiao,Jian; Su,Bojin; Zhou,Fuling; Wei,Yongchang

doi:10.3892/ijo.2016.3425

A novel curcuminoid exhibits enhanced antitumor activity in nasopharyngeal carcinoma

Authors:
- Yunbao Pan
- Guohong Liu
- Jian Xiao
- Bojin Su
- Fuling Zhou
- Yongchang Wei
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Affiliations: Department of Pathology, Affiliated Hospital, Jiangnan University, Wuxi, Jiangsu 214062, P.R. China, School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, P.R. China, Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, P.R. China, Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
Published online on: March 7, 2016 https://doi.org/10.3892/ijo.2016.3425
Pages: 2175-2183

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Abstract

Curcumin shows growth-inhibition against tumor cells through multi-target mechanisms. Nevertheless, the poor stability and pharmacokinetics considerably limit its clinical functions. Increased effort has been put into the chemical alteration of curcumin to find potential analogues with improved bioavailability and antitumor activities. In this study, the antitumor activity of a novel curcuminoid (B63) in nasopharyngeal carcinoma (NPC) was examined. The MTT and colony formation assays were used to detect NPC cell viability and proliferation. Flow cytometry was used to detect cell cycle distribution. The Annexin V/PI staining assay and cleavage PARP and cleavage caspase-3 expression were used to examine apoptosis. Western blotting was used to examine the protein expression of endoplasmic reticulum (ER) stress pathway markers, XBP-1, ATF-4 and CHOP. The suppressive effect of B63 on tumor growth was examined in vivo by subcutaneously inoculated NPC in a tumor model using nude mice. Treatment with B63 potentially caused growth inhibition and apoptosis in NPC cells in a dose- and time-responsive manner. Its antitumor effect was associated with the ER stress activation. Nevertheless, the same dose of curcumin did not activate ER stress. In addition, knockdown of Chop attenuated B63-induced cell viability inhibition, suggesting that the apoptotic pathway is ER stress-dependent. The tumor volume and weight were significantly reduced by pretreating the NPC cells with B63 before implantation in the in vivo mouse model. B63 exhibited a more potent antitumor action than curcumin in NPC. These observations on the novel compound B63 indicate a novel candidate for NPC therapy.

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