Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system

  • Authors:
    • Hiroto Izumi
    • Shohei Nagao
    • Shinichi Mochizuki
    • Nobuaki Fujiwara
    • Kazuo Sakurai
    • Yasuo Morimoto
  • View Affiliations

  • Published online on: March 23, 2016     https://doi.org/10.3892/ijo.2016.3451
  • Pages: 2472-2478
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Abstract

Silencing Y-box binding protein 1 (YB-1) can be an excellent target for cancer therapy and many lung cancer cells express the polysaccharide-recognition receptor Dectin-1. We designed a Dectin-1 targeting vehicle delivering YB-1-antisense DNA. First, we selected five optimal antisense DNA sequences to silence YB-1 from among 153 candidates. We chose the sequence closest to the start codon (AS014), and attached dA40 to the 3' end; dA40 promotes complex formation with a β-(1➝3)-d-glucan called schizophyllan (SPG). The resultant complexes were applied to 12 human-oriented lung cancer cell lines, and cell viability was examined. The cell lines exhibited decreased viability and showed strong affinity to bind SPG, suggesting the AS014/SPG complex entered the cells via the Dectin-1 mediated pathway.
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June-2016
Volume 48 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Izumi H, Nagao S, Mochizuki S, Fujiwara N, Sakurai K and Morimoto Y: Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system. Int J Oncol 48: 2472-2478, 2016.
APA
Izumi, H., Nagao, S., Mochizuki, S., Fujiwara, N., Sakurai, K., & Morimoto, Y. (2016). Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system. International Journal of Oncology, 48, 2472-2478. https://doi.org/10.3892/ijo.2016.3451
MLA
Izumi, H., Nagao, S., Mochizuki, S., Fujiwara, N., Sakurai, K., Morimoto, Y."Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system". International Journal of Oncology 48.6 (2016): 2472-2478.
Chicago
Izumi, H., Nagao, S., Mochizuki, S., Fujiwara, N., Sakurai, K., Morimoto, Y."Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system". International Journal of Oncology 48, no. 6 (2016): 2472-2478. https://doi.org/10.3892/ijo.2016.3451