Hypoxia upregulates Malat1 expression through a CaMKK/AMPK/HIF-1α axis

  • Authors:
    • Sandrine Sallé-Lefort
    • Stéphanie Miard
    • Marc-André Nolin
    • Louise Boivin
    • Marie-Ève Paré
    • Richard Debigaré
    • Frédéric Picard
  • View Affiliations

  • Published online on: July 25, 2016     https://doi.org/10.3892/ijo.2016.3630
  • Pages: 1731-1736
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Abstract

Increased expression levels of the long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (Malat1) have been associated with enhanced proliferation and metastasis of several cancer cell types. Hypoxia, a hallmark characteristic of solid tumors, has been linked to an increase in the activity of the ATP-generating AMPK protein. Since Malat1 was recently shown to be upregulated during hypoxia, the objective of this study was to determine the contribution of AMPK in the mechanistic pathways regulating Malat1 expression in low oxygen conditions. Compared to those cultured in 21% O2 conditions, HeLa cells incubated in 1.5% O2 expressed more Malat1 transcripts. This observation was mimicked in HEK293T cells using a synthetic reporter construct containing 5.6 kb of the human Malat1 promoter, suggesting that hypoxia directly impacted Malat1 gene transcription. Interestingly, pharmacological stimulation of AMPK increased Malat1 promoter transactivation in 21% O2 conditions, whereas inhibition of either AMPK or its upstream activator CaMKK completely abolished the augmentation of Malat1 under hypoxia. Pharmacological modulation of LKB1, another major regulator of AMPK, had no impact on Malat1 promoter transactivation, suggesting that calcium inputs are important in the control of Malat1 expression by AMPK. Overexpression of hypoxia-inducible factor-1α (HIF-1α) increased Malat1 expression in 21% O2 conditions, whereas pharmacological inhibition of HIF-1α blocked the impact of hypoxia on the Malat1 promoter. Taken together, these findings strongly suggest that Malat1 expression is regulated in hypoxic conditions by a CaMKK/AMPK/HIF-1α axis. More research is needed in physiological settings to test the clinical relevance of this pathway.
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October-2016
Volume 49 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sallé-Lefort S, Miard S, Nolin M, Boivin L, Paré M, Debigaré R and Picard F: Hypoxia upregulates Malat1 expression through a CaMKK/AMPK/HIF-1α axis. Int J Oncol 49: 1731-1736, 2016.
APA
Sallé-Lefort, S., Miard, S., Nolin, M., Boivin, L., Paré, M., Debigaré, R., & Picard, F. (2016). Hypoxia upregulates Malat1 expression through a CaMKK/AMPK/HIF-1α axis. International Journal of Oncology, 49, 1731-1736. https://doi.org/10.3892/ijo.2016.3630
MLA
Sallé-Lefort, S., Miard, S., Nolin, M., Boivin, L., Paré, M., Debigaré, R., Picard, F."Hypoxia upregulates Malat1 expression through a CaMKK/AMPK/HIF-1α axis". International Journal of Oncology 49.4 (2016): 1731-1736.
Chicago
Sallé-Lefort, S., Miard, S., Nolin, M., Boivin, L., Paré, M., Debigaré, R., Picard, F."Hypoxia upregulates Malat1 expression through a CaMKK/AMPK/HIF-1α axis". International Journal of Oncology 49, no. 4 (2016): 1731-1736. https://doi.org/10.3892/ijo.2016.3630