Open Access

Regulation of TPD52 by antitumor microRNA-218 suppresses cancer cell migration and invasion in lung squamous cell carcinoma

  • Authors:
    • Tomohiro Kumamoto
    • Naohiko Seki
    • Hiroko Mataki
    • Keiko Mizuno
    • Kazuto Kamikawaji
    • Takuya Samukawa
    • Keiichi Koshizuka
    • Yusuke Goto
    • Hiromasa Inoue
  • View Affiliations

  • Published online on: September 13, 2016     https://doi.org/10.3892/ijo.2016.3690
  • Pages: 1870-1880
  • Copyright: © Kumamoto et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The development of targeted molecular therapies has greatly benefited patients with lung adenocarcinomas. In contrast, these treatments have had little benefit in the management of lung squamous cell carcinoma (lung SCC). Therefore, new treatment options based on current genomic approaches are needed for lung SCC. Aberrant microRNA (miRNA) expression has been shown to promote lung cancer development and aggressiveness. Downregulation of microRNA-218 (miR-218) was frequently observed in our miRNA expression signatures of cancers, and previous studies have shown an antitumor function of miR-218 in several types of cancers. However, the impact of miR-218 on lung SCC is still ambiguous. The present study investigated the antitumor roles of miR-218 in lung SCC to identify the target genes regulated by this miRNA. Ectopic expression of miR-218 greatly inhibited cancer cell migration and invasion in the lung SCC cell lines EBC-1 and SK-MES-1. Through a combination of in silico analysis and gene expression data searching, tumor protein D52 (TPD52) was selected as a putative target of miR-218 regulation. Moreover, direct binding of miR-218 to the 3'-UTR of TPD52 was observed by dual luciferase reporter assay. Overexpression of TPD52 was observed in lung SCC clinical specimens, and knockdown of TPD52 significantly suppressed cancer cell migration and invasion in lung SCC cell lines. Furthermore, the downstream pathways mediated by TPD52 involved critical regulators of genomic stability and mitotic checkpoint genes. Taken together, our data showed that downregulation of miR-218 enhances overexpression of TPD52 in lung SCC cells, promoting cancer cell aggressiveness. Identification of tumor-suppressive miRNA-mediated RNA networks of lung SCC will provide new insights into the potential mechanisms of the molecular pathogenesis of the disease.
View Figures
View References

Related Articles

Journal Cover

November-2016
Volume 49 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kumamoto T, Seki N, Mataki H, Mizuno K, Kamikawaji K, Samukawa T, Koshizuka K, Goto Y and Inoue H: Regulation of TPD52 by antitumor microRNA-218 suppresses cancer cell migration and invasion in lung squamous cell carcinoma. Int J Oncol 49: 1870-1880, 2016.
APA
Kumamoto, T., Seki, N., Mataki, H., Mizuno, K., Kamikawaji, K., Samukawa, T. ... Inoue, H. (2016). Regulation of TPD52 by antitumor microRNA-218 suppresses cancer cell migration and invasion in lung squamous cell carcinoma. International Journal of Oncology, 49, 1870-1880. https://doi.org/10.3892/ijo.2016.3690
MLA
Kumamoto, T., Seki, N., Mataki, H., Mizuno, K., Kamikawaji, K., Samukawa, T., Koshizuka, K., Goto, Y., Inoue, H."Regulation of TPD52 by antitumor microRNA-218 suppresses cancer cell migration and invasion in lung squamous cell carcinoma". International Journal of Oncology 49.5 (2016): 1870-1880.
Chicago
Kumamoto, T., Seki, N., Mataki, H., Mizuno, K., Kamikawaji, K., Samukawa, T., Koshizuka, K., Goto, Y., Inoue, H."Regulation of TPD52 by antitumor microRNA-218 suppresses cancer cell migration and invasion in lung squamous cell carcinoma". International Journal of Oncology 49, no. 5 (2016): 1870-1880. https://doi.org/10.3892/ijo.2016.3690