Chaperonin-containing T‑complex protein 1 subunit 8 promotes cell migration and invasion in human esophageal squamous cell carcinoma by regulating α-actin and β-tubulin expression

Yang,Xiaojing; Ren,Hanru; Shao,Yuhui; Sun,Yi; Zhang,Lihua; Li,Hongling; Zhang,Xiulong; Yang,Xinmiao; Yu,Weiwei; Fu,Jie

doi:10.3892/ijo.2018.4335

Chaperonin-containing T‑complex protein 1 subunit 8 promotes cell migration and invasion in human esophageal squamous cell carcinoma by regulating α-actin and β-tubulin expression

Authors:
- Xiaojing Yang
- Hanru Ren
- Yuhui Shao
- Yi Sun
- Lihua Zhang
- Hongling Li
- Xiulong Zhang
- Xinmiao Yang
- Weiwei Yu
- Jie Fu
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Affiliations: Department of Radiation Oncology, Shanghai Jiao Tong University Affiliated with Sixth People's Hospital, Shanghai 200233, P.R China, Department of Orthopedics, Shanghai Pudong Hospital, Fudan University, Pudong Medical Center, Shanghai 201300, P.R China
Published online on: March 27, 2018 https://doi.org/10.3892/ijo.2018.4335
Pages: 2021-2030

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Abstract

The chaperonin-containing T‑complex protein 1 (CCT) has eight subunits, CCT 1-8, which are dysregulated in several types of cancer. To determine how subunit 8 (CCT8) influences the development of esophageal squamous cell carcinoma (ESCC), immunohistochemistry and western blot analysis were performed on 128 ESCC samples in the present study to measure the expression of CCT8. The prognostic value of CCT8 was analyzed using univariate and multivariate survival analyses. CCT8 knockdown in ESCC cells was performed and subsequently, the migration and invasion of ESCC cells was assessed. The results of immunohistochemistry and western blot analysis of ESCC tissue indicated that the expression of CCT8 in tumor tissues from patients with lymph node metastasis (LNM) was high whereas its expression in tissues from those without LNM was low. In addition, the overall survival rate of patients with high CCT8 expression was poor. It was demonstrated that CCT8 influenced the migration and invasion of ESCC cells by regulating α-actin and β-tubulin. Following CCT8 knockdown, cells were treated with cisplatin; it was demonstrated that α-actin and β-tubulin were downregulated and that cell apoptosis was enhanced. These data confirm that α-actin and β-tubulin are regulated by CCT8, and that increased CCT8 expression is associated with poor patient prognosis and cisplatin resistance in ESCC.

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