Inositol hexaphosphate limits the migration and the invasiveness of colorectal carcinoma cells in vitro

Schröterová,Ladislava; Ježková,Alena; Rudolf,Emil; Caltová,Kateřina; Králová,Věra; Hanušová,Veronika

doi:10.3892/ijo.2018.4488

Inositol hexaphosphate limits the migration and the invasiveness of colorectal carcinoma cells in vitro

Authors:
- Ladislava Schröterová
- Alena Ježková
- Emil Rudolf
- Kateřina Caltová
- Věra Králová
- Veronika Hanušová
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Affiliations: Department of Medical Biology and Genetics, Charles University, Faculty of Medicine in Hradec Králové, 500 03 Hradec Králové, Czech Republic, Department of Biochemical Sciences, Charles University, Faculty of Pharmacy, 500 05 Hradec Králové, Czech Republic
Published online on: July 19, 2018 https://doi.org/10.3892/ijo.2018.4488
Pages: 1625-1632

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Abstract

Inositol hexaphosphate (IP6), also known as phytic acid, has been shown to exhibit anticancer effects in a number of preclinical tumor models. IP6 decreases proliferation by arresting cells in the G0/G1 phase, inhibits iron-mediated oxidative reactions, enhances differentiation and stimulates apoptosis. The present study attempted to characterize the effect of IP6 on the migration and adhesion of colon cancer SW620 cells. IP6 was assessed at concentrations of 0.2 and 1 mM during 12, 24 and 48 h of exposure. Migration ability was measured with the real-time xCELLigence Real-Time Cell Analyzer Dual Purpose system. The expression of mRNA and proteins involved in migration and cancer progression [epithelial cell adhesion molecule, intercellular adhesion molecule-1, β-catenin, N-cadherin, E-cadherin, matrix metalloproteinase (MMP)-2 and MMP-9] was determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis. The changes in the expression and subcellular localization of E-cadherin were determined by indirect immunofluorescence. IP6 induced a decrease in the migration ability of the tested SW620 cell line. IP6-treated cells also showed decreased expression of N-cadherin, increased levels of E-cadherin and decreased expression of MMP-2 and MMP-9. These results indicated that IP6 has potential to modulate the migration ability and expression of markers associated with invasion in SW620 cells; however, further analysis is necessary to obtain a detailed understanding of the mechanism of action.

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