Hematopoietic rescue in mice via alpha 1-adrenoceptors on bone marrow B cell precursors

  • Authors:
    • M Togni
    • G Maestroni
  • View Affiliations

  • Published online on: August 1, 1996     https://doi.org/10.3892/ijo.9.2.313
  • Pages: 313-318
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We demonstrated that adrenergic agents may affect hematopoiesis via high and low affinity al-adrenergic receptors (alpha 1-ARs) present on bone marrow cells. Here we show that the high affinity, alpha 1-AR is present on Mac1(-) B220(+)sIgM(-) (pre-B) cells. The low affinity alpha 1-AR seems to be present on Mac1(+) B220(-) cells. Noradrenaline administration in mice rescued hematopoiesis from the toxic effect of carboplatin or X-rays sublethal irradiation. The protection was reflected by higher leukocyte and platelets counts as well as by increased bone marrow granulocyte/macrophage colony-forming units (GM-CFU). At its most effective dose (3 mg/kg, s.c.), noradrenaline protected 77% of the mice injected i.v. with 200 mg/kg of carboplatin (LD 100:170 mg/kg). The contemporary administration of the alpha 1-AR antagonist prazosin brought the percent of surviving mice down to 30% indicating that alpha 1-ARs mediated most of the noradrenaline-induced hematopoietic rescue. In vitro, noradrenaline (1 mu M) rescued GM-CFU in unseparated bone marrow cells containing the adherent population expressing the high affinity alpha 1-AR. Consistently, the hematopoietic rescue was counteracted by low concentrations (0.1 nM-10 nM) of the alpha 1-antagonist prazosin. Our findings describe a novel mechanism of hematopoietic regulation and might find application in preventing the myeloablative effect of anticancer treatments.

Related Articles

Journal Cover

August 1996
Volume 9 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Togni M and Maestroni G: Hematopoietic rescue in mice via alpha 1-adrenoceptors on bone marrow B cell precursors. Int J Oncol 9: 313-318, 1996.
APA
Togni, M., & Maestroni, G. (1996). Hematopoietic rescue in mice via alpha 1-adrenoceptors on bone marrow B cell precursors. International Journal of Oncology, 9, 313-318. https://doi.org/10.3892/ijo.9.2.313
MLA
Togni, M., Maestroni, G."Hematopoietic rescue in mice via alpha 1-adrenoceptors on bone marrow B cell precursors". International Journal of Oncology 9.2 (1996): 313-318.
Chicago
Togni, M., Maestroni, G."Hematopoietic rescue in mice via alpha 1-adrenoceptors on bone marrow B cell precursors". International Journal of Oncology 9, no. 2 (1996): 313-318. https://doi.org/10.3892/ijo.9.2.313