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Introduction

The non-invasive imaging of GLUT2-expressing cells, such as insulin-producing pancreatic islet cells, remains a challenge (1). INS-1E cells are currently used as a model of glucose-responsive insulin-producing cells (2,3). The aims of the present study were to document the presence of GLUT2 in INS-1E cells by immunocytochemistry and to explore its possible role in the concentration- and time-dependent uptake of two novel fluorescent desnitroso-streptozotocin analogs. For the purpose of comparison, similar experiments were conducted in human embryonic kidney (HEK) cells, rat isolated pancreatic islets, rat hepatic cells, rat exocrine pancreatic cells and BRIN-BD11 cells. In the INS-1E cells and other GLUT2-expressing cells, the cell fluorescence eventually reached a level suggesting that the intracellular concentration of the desnitroso-streptozotocin analogs exceeded, by approximately one order of magnitude, the extracellular concentration. It is proposed, therefore, that these fluorescent analogs may be used to label GLUT2-expressing cells.

Materials and methods

Fluorescent desnitroso-streptozotocin analogs

The first probe used in the present study (probe A) was prepared according to the methods of Ran et al(4), as follows. Trifluoroacetic acid (TFA; 100 μl) was added to a solution of 1,3,4,6-tetra-O-acetyl-2-[3-(4-tert-butoxycarbonylamino-butyl)-ureido]-2-deoxy-glucopyranose (Compound 1 in Fig. 1; 4.4 mg, 8.0 mmol) in dichloromethane (100 μl) and the resultant mixture was stirred at room temperature for 2 h. After evaporation of the solvent and TFA, an oily residue was obtained. Cy5.5-NHS (1.0 mg), water (100 μl), acetonitrile (100 μl) and triethylamine (10.0 mg) were added to this residue. The resulting mixture was stirred at room temperature for 4 h, and this was followed by the addition of sodium methoxide (108.0 mg) in methanol (100 μl). The resultant mixture was stirred at room temperature overnight and the solvent was removed under vacuum to give an oily residue. This residue was subjected to a reversed-phase flash C18 column to obtain probe A (Cy5.5; 0.6 mg; 55%); ESI-MS: m/z 1192.9.

Figure 1 Fluorescent-tagged desnitroso-streptozotocin analogs.

The second probe used in the present study (probe B) was prepared by an adaptation of the method proposed by Ran et al(4) and based on the ATTO TEC procedure sheet whereby the dye to sugar ratio was optimized from 9:1 (dye:sugar) to a ratio of 4:1. TFA (500 μl) was added to a solution of 1,3,4,6-tetra-O-acetyl-2-[3-(4-tert-butoxycarbonylamino-butyl)-ureido]-2-deoxy-glucopyranose (Compound 1 in Fig. 1; 29.8 mg, 52.1 mmol) in dichloromethane (500 μl), and the resultant mixture was stirred at room temperature for 2 h. After evaporation of the solvent and TFA, an oily residue was obtained. ATTO 647N-NHS (5.0 mg, 5.9 μmol) was dissolved in 1.5 ml DMSO, and triethylamine (80 μl) was added to the residue. The resulting mixture was stirred at room temperature for 2 h and the solvent was evaporated. The residue was taken up in water and the organic phase was extracted twice with dichloromethane. The separated and combined organic layers were dried by evaporation under a vacuum. Purification was performed using Biogel chromatography in MeOH to give 4.4 mg (83%) of a blue solid. Subsequent deacetylation was performed in 2 ml MeOH and by addition of NH3 in MeOH (100 μl; 7N) and the mixture was stirred for 12 h. MALDI-TOF MS yielded a 902.9 [M+Na]+ molecular mass (MALDI-TOF MS was by a Bruker Biflex III, using 2,5-dihydroxybenzoic acid as a matrix).

INS-1E, HEK, BRIN-BD11, hepatic and pancreatic exocrine cells and isolated pancreatic islets

The insulin-producing INS-1E cells were kindly provided by Professor C. Wollheim (University Medical Center, Geneva, Switzerland) and cultured at 37ºC in RPMI-1640 medium (Invitrogen Life Technologies Europe BV, Gent, Belgium) containing 11.1 mM D-glucose and 2.0 mM L-glutamine, and supplemented with 5% (v/v) heat-inactivated fetal bovine serum, 1% (v/v) penicillin (100 U/ml)-streptomycin (100 μg/ml), 10.0 mM HEPES, 1.0 mM sodium pyruvate and 50.0 μM 2-mercaptoethanol, in humidified air containing 5% CO2. The INS-1E cells were used between passages 20 and 40.

HEK-293 cells were provided by American Type Culture Collection (ATCC, Manassas, VA, USA). The HEK cells were incubated in the ATCC formulated Eagle’s minimum essential medium, to which fetal bovine serum (final concentration 10%), penicillin (50 IU/ml) and streptomycin (50 μg/ml) were added.

Rat pancreatic islets were isolated by the collagenase procedure (5), whilst rat pancreatic exocrine cells were isolated according to the procedure proposed by Amsterdam and Jamieson (6). The islets and exocrine cells were incubated in RPMI-1640 culture medium (Invitrogen Life Technologies). The study was approved by the Ethic Committee of Brussels Free university (Brussels, Belgium).

Rat hepatic cells were prepared according to the method of Berry and Friend (7) and incubated in DMEM medium (Gibco-BRL, Invitrogen Life Technologies) containing 10.0 mM D-glucose, L-glutamine, penicillin, streptomycin and 10% inactivated fetal bovine serum.

BRIN-BD11 cells were kindly provided by Professor A. Herchuelz (Laboratory of Pharmacology, Brussels Free University, Brussels, Belgium) and cultured as described elsewhere (8).

Immunohistochemistry

INS-1E cells were plated on 13 mm glass coverslips, placed into 12-well plates and left to adhere and proliferate for 4 days in RPMI GlutaMAX™ medium (Gibco-BRL, Invitrogen) supplemented with 1 mM sodium pyruvate (Gibco-BRL), penicillin-streptomycin (100 U/ml and 100 μg/ml, Gibco-BRL), 50 μM β-mercaptoethanol and 10% FBS. Prior to staining, cells were washed with PBS, then fixed in 4% PAF (v/v) for 15 min and washed several times with TBS and TBS-Triton 0.3% (v/v). Coverslips were covered with 10% normal goat serum for 1 h at room temperature, followed by incubation with rabbit anti-GLUT2 antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA; sc-9117; dilution 1:100), overnight at 4ºC. The secondary antibody was a goat anti-rabbit conjugated to Alexa594 (Invitrogen; dilution 1:100). The nuclei were counterstained with DAPI. Coverslips were examined using the Axioplan microscope (Carl Zeiss, Göttingen, Germany) connected to a video camera and a complementary acquisition system.

Uptake of fluorescent desnitroso-streptozotocin analogs

In order to assess the uptake of probes A and B, groups of ~70×103 cells or 15 rat isolated pancreatic islets were incubated for 15 min or more, up to 24 h, in 0.1 ml of incubation medium. After incubation, the extracellular medium was removed and after two washes, the fluorescence of the cells (or islets) was measured using a Glomax Multi Detection instrument (Promega, Leiden, The Netherlands). The results of these measurements were expressed in arbitrary units.

The reference fluorescence values measured at increasing concentrations (0.15, 0.3, 0.6 and 1.2 μM) of probe A or B, and expressed in arbitrary units, yielded a positive correlation (P<0.03 or less) with the latter concentrations. In two experiments conducted in the 0.15–1.2 or 0.3–2.0 μM range, the A/B ratios found at increasing concentrations averaged 55.1±6.6% (n=8). At increasing concentrations of each probe, the fluorescent measurements were not strictly proportional to the concentration of the probe (Fig. 2). For instance, the measurements recorded at the lowest concentration of the probes represented 25.2±4.5% (n=4) of the corresponding value found within the same experiment and with the same probe at a 6–7 times higher concentration, distinct (P<0.05) from the theoretical value of 13.8±0.7% (n=4) based on the assumption of a rule of proportionality.

Figure 2 Fluorescence of probe standards in a representative experiment. The fluorescence (F) of probe A (left) and probe B (right) at increasing probe concentrations (0.15–0.3–0.6–1.2 μM) is illustrated in the inset graph (crosses and dashed line). The differences between the estimated maximal fluorescence (Fmax) and F, on a logarithmic scale, are illustrated in the main panels (circles and solid line), the oblique solid line corresponding to the regression line.

Fluorescence imaging

Cells (or isolated pancreatic islets) were seeded on 96-well cell culture plates (Greiner Bio-One, Alphen aan den Rijn, The Netherlands). After incubation, the probe-containing medium was removed and the plates were washed twice.

The fluorescence imaging of labeled cells was achieved using an Axiovert 200m microscope (Carl Zeiss NV-SA) and a TexaRed filter.

D-glucose phosphorylation in INS-1E cell homogenates

Groups of 2×106 INS-1E cells were sonicated three times for 10 sec in 1.0 ml of an iced Hepes-NaOH buffer (75.0 mM, pH 7.4) containing 150.0 mM KCl, 15.0 mM KH2PO4, 16.5 mM MgCl2 and 1.5 mM EDTA. Aliquots (50 μl) of this homogenate were mixed with 10 μl of the same homogenization buffer and 60 μl of the same Hepes-NaOH buffer as mentioned above, but diluted with H2O in a 2/1 ratio (yielding a 50.0 mM Hepes-NaOH buffer) and enriched with ATP (20.0 mM), D-glucose (7.0 or 50.0 mM) and a trace amount of D-[U-14C]glucose. After 60 min incubation at 37ºC, 1.0 ml of cold H2O was added to each sample and the radioactive acidic metabolites were separated by anion-exchange chromatography (9).

Ethical approval

The study was approved by the Ethic Committee of Brussels Free university (Brussels, Belgium).

Statistical analysis

All results are expressed as the mean values (±SEM) together with either the number of individual determinations (n) or degree of freedom (df). The statistical significance of differences between mean values was assessed by use of Student’s t-test.

Results

Immunocytochemistry of GLUT2

As illustrated in Fig. 3, GLUT2 protein was largely detected by immunofluorescence in the membrane of INS-1E cells, but also in the cytosol of these cells.

Figure 3 INS-1E cells were stained for the glucose transporter GLUT2 (A, red) and nuclei were counterstained with DAPI (B, blue). GLUT2 protein was largely detected by immunofluorescence in the membrane of INS-1E cells (C-a, arrow) but also in the cytosol (C-b, arrowhead). Images A, B and C were captured at ×20 magnification. Details in the insets were obtained using the magnification tool of Adobe Photoshop 7.0 and correspond to ×100 magnification.

Uptake of desnitroso-streptozotocin analogs

In a representative experiment, the fluorescence of INS-1E cells not exposed to any probe did not exceed 5.1 arbitrary units/103. In the same experiment, after 24 h incubation at the highest probe concentration (12.0 μM) used in the present study, the fluorescence of INS-1E cells with probe A and B averaged, respectively, 50.4 and 247.2 arbitrary units/103. Taking into account both the reference values for the fluorescence of the probes themselves (see Materials and methods) and the volume of INS-1E cells, i.e. 1.5 nl/103(10), the latter value would yield apparent intracellular concentrations of ~78.4 μM with probe A and 217.3 μM with probe B: approximately one order of magnitude higher than the initial extracellular concentration (12.0 μM).

In a series of six experiments conducted in INS-1E cells incubated for 5 or 24 h in the presence of probe A or B in concentration ranges of 0.25–0.5–1.0 μM or 3.0–6.0–12.0 μM, the probe A/probe B ratio for the fluorescence of the cells averaged 21.8±1.8% (n=18). The data collected in cells exposed to probe A or B in the low concentration range of 0.25–0.5–1.0 μM yielded an A/B ratio of cell fluorescence (21.9±2.5%; n=6) virtually identical to that recorded in the higher concentration range of 3.0–6.0–12.0 μM (21.7±2.5%; n=12). Such ratios were markedly lower (P<0.005 or less) than those observed for the fluorescence of the probes themselves (55.1±6.6%; n=8), indicating that, under identical experimental conditions, the net uptake of probe A by the INS-1E cells, considered in molar units, only represented ~40% of that of probe B.

The A/B ratio for INS-1E cell fluorescence was not identical, however, after either 5 or 24 h incubation. Thus, the value recorded after 5 h incubation averaged 134.1±11.7% (n=9; P<0.02) of the paired value recorded within the same experiment(s) and at the same concentration of each probe after 24 h incubation. This finding suggests that a difference may prevail between the two probes in terms of the time course for their uptake by the INS-1E cells.

In agreement with the latter suggestion, the time course for the uptake of probe A or B indicated that, after 5 h incubation, the fluorescence of INS-1E cells exposed to these probes in the 3.0–6.0–12.0 μM range amounted to 89.7±2.9% (n=6) in the case of probe A and 61.6±3.5% (n=6) in the case of probe B of the paired value recorded within the same experiment(s) with the same probe and at the same probe concentration after 24 h incubation. In addition to differing significantly (P<0.001) from one another, the latter two mean percentages were both significantly lower than unity (P<0.02 or less) and both significantly higher (P<0.001) than the theoretical value of 20.8% based on the assumption of a rule of proportionality between uptake of the probe and length of incubation.

The concentration dependency for the uptake of probes A and B was examined in 3 experiments conducted in the 0.25–0.5–1.0 μM range and in 4 experiments conducted in the 3.0–6.0–12.0 μM range. The results of the latter experiments are shown in Fig. 4. The results indicate that, after 5 or 24 h incubation in the presence of either probe A or probe B, the fluorescence of the INS-1E cells averaged 3.0 and 6.0 μM, respectively, which was 37.5±6.7 and 60.7±5.4% (n=8) of the corresponding value recorded at 12.0 μM, yielding a curvilinear pattern with downward concavity (Fig. 3, right panel). When the cell fluorescence observed at 3.0 and 6.0 μM was expressed relative to the close-to-maximal value (Umax) recorded at 12.0 μM, the results were compatible with an exponential pattern responding to the equation U = Umax (1 − e−KC), in which U represents the uptake of the probe at a concentration (C) and K the slope of the corresponding regression line established in semi-logarithmic coordinates. Thus, as shown in Fig. 4 (left panel), the regression line between the decimal logarithmic values of (Umax - U) and the concentration of the probe yielded a correlation coefficient equal to unity and a negative slope of −0.0676 μM−1. At lower probe concentrations in the 0.25–0.5–1.0 μM range, the measurements of INS-1E cell fluorescence also yielded a curve with downward concavity, and the results recorded at 0.25 and 0.5 μM averaged 35.1±8.7% (n=4) and 71.3±8.1% (n=4), respectively, of the paired corresponding value observed under the same experimental conditions (30 min, 5 h and 24 h incubation) at the 1.0 μM concentration of the same probe. These percentages did not differ significantly from those expected from the regression line drawn in the left panel of Fig. 4.

Figure 4 Right panel: correlation between the uptake of probes A and B (U), as judged from the fluorescence of INS-1E cells incubated for either 5 or 24 h, and the probe concentration. Mean values (±SEM) refer to 8 individual measurements, and are expressed relative to the paired value found for the same probe after the same time of incubation in the presence of a 12.0 μM concentration (100%). Left panel: semi-logarithmic presentation of the relationship between Umax – U and probe concentration; the experimental data are the same as in the right panel, the close-to-maximal value (Umax) being taken as that recorded at a 12.0 μM probe concentration (100%). The oblique line corresponds to the regression line.

When compared within the same experiment(s) and after 5 or 24 h incubation, there was little difference in the fluorescence of INS-1E cells exposed to either probe A or probe B (3.0, 6.0 and 12.0 μM) and incubated at either low (2.8 mM) or high (16.7 mM) concentrations of D-glucose, with a paired 2.8 mM/16.7 mM ratio averaging 109.3±16.4% (n=12; P>0.59 vs. unity). Thus, there was no evidence that the uptake of either probe A or probe B was affected by the concentration of D-glucose in the incubation medium.

The uptake of probes A and/or B by INS-1E cells was also compared to that found, under comparable experimental conditions, in HEK-293 cells, taken as representative of GLUT2-expressing kidney cells (11), rat pancreatic islets, rat hepatocytes, rat exocrine pancreatic cells and BRIN-BD11 cells.

The fluorescence measurements observed in the HEK-293 cells yielded information closely comparable to that collected in the INS-1E cells. Firstly, whether in HEK cells exposed for either 5 or 24 h to either probe A or probe B, their respective uptake at 12.0 μM was 2.85±0.34 times higher (n=4; P<0.02) than the paired value observed at a 3.0 μM concentration, indicating a lack of proportionality (P<0.05) between uptake and concentration. Furthermore, the 3.0/12.0 μM ratio for the fluorescence of HEK cells averaged 36.8±4.8% (n=4), a value almost identical (P>0.94) to that computed in the INS-1E cells (37.5±6.7%; n=8). Secondly, the paired probe A/probe B ratio for the fluorescence of HEK cells averaged 23.3±2.6% (n=4), a value again almost identical (P>0.71) to that found in INS-1E cells (21.8±1.8%; n=18). Lastly, the fluorescence of HEK cells exposed for 5 h to probes A or B (3.0 or 12.0 μM) represented 50.7±5.0% (n=4) of the paired value recorded after 24 h of incubation, such a percentage being significantly higher (P<0.01) than the theoretical value of 20.8% based on the assumption of a rule of proportionality between probe uptake and length of incubation, as also observed in INS-1E cells.

In rat isolated pancreatic islets exposed to probe A or B (3.0, 6.0 and 12.0 μM), the fluorescence measurements recorded after only 15 min incubation indicated that the net uptake of these probes had already reached its close-to-equilibrium value. Thus, the values observed after only 15 min incubation averaged 106.4±1.4% (n=6) of the corresponding measurements made after 24 h incubation. As judged from the comparison of measurements made in the islets at increasing concentrations of each probe (3.0, 6.0 and 12.0 μM), the concentration-uptake relationship differed with probe A, as compared to probe B. In the case of probe A, the uptake was grossly proportional to the concentration. For instance, whether after 15 min or 24 h incubation, it represented 22.3±1.8% and 50.6±1.1%, at 3.0 and 6.0 μM, respectively, of the corresponding value found at 12.0 μM. In the case of probe B, the measurements at 3.0 μM averaged 73.2±2.1% of those recorded at 6.0 μM, the latter measurement representing 70.9±0.2% of that found at 12.0 μM. The results obtained in the islets with probe B are thus similar to those illustrated in Fig. 4, with a negative coefficient of correlation between log10 (Umax – U) and concentration (0, 3.0 and 6.0 μM) amounting to −0.9943 and the slope of the corresponding regression line yielding a negative value of −0.0894 μM−1. As expected from these findings, the probe A/probe B paired ratio for islet fluorescence, as computed after either 15 min or 24 h incubation, increased from 38.0±1.4% at 3.0 μM to 63.0±0.2% at 6.0 μM and 88.3±2.0% at 12.0 μM.

After 30 min incubation in the presence of probe B tested in the 0.25–0.5–1.0 μM range, the concentration-dependent fluorescence of rat hepatic cells and INS-1E cells, assessed within the same experiment, yielded a comparable relationship. Thus, according to the analytical procedure illustrated in Fig. 3, the slope of the regression line (± sample standard deviation of the regression coefficient) was almost identical (P>0.87) in hepatocytes (0.093±0.010 μM−1) and INS-1E cells (0.091±0.009 μM−1) with a correlation coefficient between log10 (Umax – U) and C amounting, respectively, to 0.9912 in hepatocytes (n=4; P<0.009) and 0.9850 in INS-1E cells (n=4; P<0.02). The absolute value for fluorescence, however, was 4–5 times higher in hepatic cells than in INS-1E cells.

Within the same experiment, the results collected in rat exocrine pancreatic cells, which do not express GLUT2 and were incubated under the same experimental conditions, differed from those found in either hepatic cells or INS-1E cells in several respects. Firstly, the dispersion of individual measurements at increasing concentrations of probe B was greater in rat exocrine pancreatic cells than in either rat liver cells or INS-1E cells, as documented by the lower correlation coefficient between cell fluorescence and probe concentration in the exocrine cells (r=0.8686; n=6; P<0.03) than in the liver cells (r=0.8969; n=6; P<0.001) or INS-1E cells (r=0.9757; n=6; P<0.001), and by the much higher magnitude of the standard deviation of the regression coefficient between cell fluorescence and probe concentration, expressed relative to the latter coefficient, in exocrine cells (57.1%) than in either liver cells (16.4%) or INS-1E cells (22.5%). Secondly, at any given probe concentration, the fluorescence of exocrine cells was markedly lower than that of liver cells or INS-1E cells. Expressed in arbitrary units, the slope of the regression line between cell fluorescence and probe concentration did not exceed 1.86±1.06 103/μM, a value ~15 times lower than that found for hepatic cells, 28.28±4.63 103/μM. The slope found in the exocrine cells was also 2–3 times lower than that found in the INS-1E cells (4.25±0.95 103/μM), within the same experiment. The statistical significance of the difference was confirmed by covariance analysis (F=11.3, f=1, 8; P=0.01).

The possible role of distinct glucose transporters in the uptake of the two probes examined in this study was supported by findings collected in BRIN-BD11 cells established by electrofusion of RINm5F cells with New England Deaconess Hospital (NEDH) rat pancreatic islet cells (12). The metabolism of D-glucose in the BRIN-BD11 cells displays several analogies with that of the hexose in the parent RINm5F cells (13). For instance, the concentration dependency of D-[5-3H]glucose utilization is almost identical in RINm5F and BRIN-BD11 cells. The total energy yield from D-glucose catabolism is also similar in these two cell lines (13). The RINm5F cells have lost an essential attribute of the glucose sensor device in normal insulin-producing cells, namely the capacity to ensure the equilibrium of D-glucose concentrations across the plasma membrane (14), coinciding with a severely impaired uptake of the diabetogenic agent alloxan and with resulting resistance to its cytotoxic action (15). A comparable situation may prevail in BRIN-BD11 cells. In turn, such a situation may account for the unusual concentration dependency for the uptake of probes A and B by BRIN-BD11 cells with either an upward concavity (probe A) or sigmoidal pattern (probe B), as shown in Fig. 5. In these experiments, the increase in the fluorescence of the BRIN-BD11 cells in the low range of probe concentrations, expressed relative to the increment in probe concentrations, represented no more than 16.2±4.8% (n=3) of that recorded with the same probe in the steepest segments of the curves shown in Fig. 5. Moreover, whilst the probe A/probe B ratio in cell fluorescence amounted to 85.8% at the lowest probe concentration (3.6 μM), it reached a value two times lower (42.5%) at the highest probe concentration (12.0 μM).

Figure 5 BRIN-BD11 cell fluorescence after 24 h incubation in the presence of increasing concentrations of either probe A (closed circles and dashed line) or probe B (open circles and dotted line).

At the lowest extracellular probe concentration used in these experiments (3.0 μM), their estimated intracellular concentration, taking into account a BRIN-BD11 cell volume of 1.60 nl/103 cells (8), was close to 16.5 and 10.9 μM for probe A and B, respectively, as compared to corresponding values of 20.6 and 76.0 μM in INS-1E cells. The marked difference observed under these experimental conditions in terms of the apparent uptake of probe B by BRIN-BD11 cells versus INS-1E cells was reduced at the highest probe concentration (12.0 μM) with estimated intracellular concentrations of 135.6 μM (probe A) and 180.3 μM (probe B) in BRIN-BD11 cells, as compared to 78.4 μM (probe A) and 217.3 μM (probe B) in INS-1E cells.

Fluorescent imaging

Even at a 1.0 μM concentration of probe B, uptake could not be documented by fluorescence imaging in rat exocrine pancreatic cells (Fig. 6), in contrast with the findings made in rat pancreatic islets (Fig. 7), rat liver cells (Fig. 8) and INS-1E cells (data not shown).

Figure 6 Fluorescence imaging of rat pancreatic exocrine cells incubated for 30 min with probe B (1.0 μM).

Figure 7 Fluorescence imaging of rat pancreatic islets incubated for 30 min with probe B at (A) 3.0 μM or (B) 6.0 μM.

Figure 8 Fluorescence imaging of rat hepatocytes incubated for 30 min with probe B at (A) 0.5 μM and (B) 1.0 μM.

D-glucose phosphorylation

At 3.5 and 25.0 mM D-glucose, the phosphorylation of hexose by INS-1E cell homogenates averaged, respectively, 13.8±1.1 and 55.8±2.1 pmol/103 cells per 60 min (n=8–9). The latter values were not significantly different (P>0.34 or more) from the conversion of D-[5-3H]glucose to 3HOH by intact INS-1E cells incubated at either 2.8 mM D-glucose (11.1±3.2 pmol/103 cells per 60 min) or 16.7 mM D-glucose (46.3 ± 5.7 pmol/103 cells per 60 min), as previously reported (16).

Discussion

The present study documents the presence of both GLUT2 and a high Km glucokinase-like catalytic activity in INS-1E cells. These attributes are well suited to account for the glucose-sensing capacity of these cells (3). The high similarity between the rate of D-glucose phosphorylation by INS-1E cell homogenates and that of D-[5-3H]glucose utilization by intact cells supports the view that the concentration of D-glucose in the cytosol of INS-1E cells is close to its extracellular concentration and that the glucokinase-like enzyme represents the key determinant of D-glucose phosphorylation and further catabolism.

The labeling of insulin-producing cells remains a challenge, as recently reviewed (1). The present study dealt mainly with the use of fluorescent desnitroso-streptozotocin analogs using a more hydrophilic and a more hydrophobic dye component. It may be objected that this approach is restricted to the ex vivo labeling of insulin-producing cells. However, it should not be ruled out that the detection and quantification of pancreatic β-cells in vivo could be achieved using the present fluorescent probes and a suitable fluorescence detection instrument transiently inserted in the peritoneal cavity.

The concentration- and time-dependent fluorescence of cells exposed to either probe A or B yielded comparable results in rat isolated pancreatic islets and either INS-1E, HEK or hepatic cells, whilst a different situation prevailed in rat exocrine pancreatic cells or BRIN-BD11 cells. Moreover, a pronounced fluorescence in the cells exposed to either probe A or B was rapidly reached despite the fact that the extracellular concentration of these probes, as tested in the present study, remained in the 0.15–1.2 μM range. Differences in the cell response to probe A and probe B, as documented in the present study, may be accounted for by their different chemical structure and hydrophobicity.

In conclusion, the present study provides novel findings considered as potentially helpful in the labeling of GLUT2-expressing cells with a fluorescent probe.

Acknowledgements

This study was supported by the European Community’s Seventh Framework Programme FP7/2007-2013-FP7-NMP-In Vivo Imaging of Beta-cell by Applied Nano Technology under grant agreement No. (228-993)-(VIBRANT).

References