Protective effect of mitochondria‑targeted peptide MTP‑131 against oxidative stress‑induced apoptosis in RGC‑5 cells
- Authors:
- Published online on: March 1, 2017 https://doi.org/10.3892/mmr.2017.6271
- Pages: 2179-2185
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Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Previous studies have demonstrated the importance of mitochondria and oxidative stress in the development of neuronal diseases, such as Parkinson's disease (PD) and Alzheimer's disease (1,2). In human eyes, the retina is extremely vulnerable to oxidative damage. Oxidative stress has been demonstrated to be the predominant mechanism associated with the pathogenesis of age-related macular degeneration (AMD), diabetic retinopathy (DR), glaucoma and retinitis pigmentosa (RP) (3–6). Novel strategies targeting mitochondria and reactive oxygen species (ROS) generation may provide promising prospects towards antioxidant therapy in these retinal diseases.
A novel mitochondria-targeted peptide, MTP-131 (also known as SS-31 or Bendavia), originally designed by Hazel H. Szeto and Peter W. Schiller (7), is a water-soluble peptide with an alternating aromatic cationic motif (D-Arg-dimethylTyr-Lys-Phe-NH2) and a 3+ net charge at physiological pH (8). The fraction of the peptide partitioned to mitochondria has been estimated to be 1,000 to 5,000-fold compared with the extra-mitochondrial concentration, and the peptide is predominately localized to the inner mitochondrial membrane (7). MTP-131 reduces ROS production in a dose-dependent manner and prevents oxidative damage in neuronal cell lines (9). In addition, treatment of mice with MTP-131 prevents loss of neurons and increases survival in PD and amyotrophic lateral sclerosis animal models (10).
Since MTP-131 has a unique mitochondria-targeted delivery, and exhibits potent antioxidant and neuroprotective effect both in vitro and in vivo, the present study aimed to evaluate the protective effect of MTP-131 against hydrogen peroxide (H2O2)-induced oxidative damage in RGC-5 cells.
Materials and methods
Reagents and chemicals
MTP-131 (D-Arg-(2′6′-dimethylTyr)-Lys-Phe-NH2) was kindly provided by Stealth BioTherapeutics, Inc. (Newton, MA, USA). Gibco Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Lactate dehydrogenase (LDH) assay kit was obtained from Roche Diagnostics Gmbh (Mannheim, Germany). Tetramethylrhodamine methyl ester perchlorate (TMRM), 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and MitoTracker Deep Red FM were obtained from Thermo Fisher Scientific, Inc. and prepared as 50 µM, 1 and 1 mM stock solutions in dimethyl sulfoxide, respectively. Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis assay kit was from Multisciences Lianke Biotech Co., Ltd. (Hangzhou, China). Unless specified, all other reagents were obtained from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany).
Cell culture
The RGC-5 cell line was kindly provided by Dr Zhiqun Tan (Department of Neurology, UC Irvine School of Medicine, Irvine, CA, USA). Undifferentiated RGC-5 cells were used in the present study. Cells were cultured in DMEM containing 10% FBS, and 1% penicillin-streptomycin (100 U/ml penicillin and 100 µg/ml streptomycin) at 37°C and 5% CO2. Cells were passaged by trypsinization every 3 days. All cell culture dishes and plates were obtained from BD Biosciences (Franklin Lakes, NJ, USA).
MTP-131 pretreatment and induction of oxidative stress
RGC-5 cells were seeded at a density of 1×104 cells/well in 6-well plates and incubated in 5% CO2 at 37°C for 24 h. Cells at approximately 70% confluence were pretreated with 0.01, 0.1 or 1 µM MTP-131 in serum-free DMEM at 37°C for 1 h and then rinsed twice with PBS. Then, RGC-5 cells were exposed to 500 µM H2O2 in serum-free DMEM for 24 h to induce a sustained oxidative stress in vitro. Untreated cells and cells treated with H2O2 alone were used as normal and H2O2 control, respectively.
Measurement of cell viability
Cell viability was assessed by LDH assay using the Cytotoxicity Detection kitPLUS (Roche Diagnostics Gmbh) according to the manufacturer's instructions and as described previously (11). Briefly, RGC-5 cells were seeded in 96-well plates at a density of 5×103/well, and treated as described above. Culture supernatant (50 µl) from each well was transferred to a new 96-well plate and 100 µl reaction mixture solution was added to each well. Following incubation at room temperature in the dark for 30 min, 50 µl of stop solution was added to each well to terminate the reaction. Absorbance was measured using a microplate reader (Bio-Rad Laboratories, Inc., Hercules, CA, USA), at 490 nm test wavelength and at 630 nm reference wavelength. Cell viability was estimated as follows: cytotoxicity (%) = (experimental value-low control) / (high control-low control) ×100%.
Measurement of mitochondrial membrane potential changes (ΔΨm)
TMRM, a cationic fluorescent probe which is taken up by mitochondria in a potential-dependent manner, was used to evaluate ΔΨm, as described previously (9). To quantify ΔΨm by flow cytometry, RGC-5 cells (1×104 cells/well in a 6-well plate) were pretreated with 0.01, 0.1 or 1 µM MTP-131 for 1 h and then incubated with 500 µM H2O2 for 2 h. Cells were harvested and suspended in freshly prepared TMRM (200 nM) in phenol red and serum-free DMEM for 30 min at 37°C in the dark. All samples were rinsed twice with PBS and analyzed immediately by flow cytometry using a FACSAria II (BD Biosciences, Franklin Lakes, NJ, USA) and excitation/emission (ex/em) wavelengths 548/573 nm. A total of 1×104 cells were routinely collected, and results were expressed in arbitrary units as the mean fluorescence intensity (MFI) from the average of at least three separate experiments.
For confocal microscopy, RGC-5 cells were plated in Petri dishes
Following MTP-131 pretreatment and H2O2 incubation, cells were rinsed twice and loaded with freshly prepared 500 nM TMRM for 30 min at 37°C in the dark. Following two rinses with PBS, cells were visualized by confocal microscopy using a LSM 510 microscope (Carl Zeiss AG, Oberkochen, Germany) and ex/m wavelengths 548/573 nm.
Measurement of intracellular ROS
Intracellular ROS in RGC-5 cells was measured with H2DCFDA, as described previously (12). This nonfluorescent probe accumulates within cells and transforms into 2′,7′-dichlorodihydrofluorescein (H2DCF) by deacetylation, which then reacts with ROS to form the fluorescent dichlorofluorescein (DCF). To quantify ROS by flow cytometry, RGC-5 cells (1×104 cells/well in a 6-well plate) were pretreated with 0.01, 0.1 or 1 µM MTP-131 for 1 h and then incubated with 500 µM H2O2 for 24 h. Cells were harvested and suspended in freshly prepared H2DCFDA (5 µM) in phenol red and serum-free DMEM for 30 min at 37°C in the dark. All samples were rinsed twice with PBS and analyzed immediately by flow cytometry (ex/em: 488/530 nm). Ten thousand cells were routinely collected, and results were expressed as the MFI in arbitrary units from the average of at least three separate experiments.
For visualization by confocal microscopy, cells were plated in Petri dishes. Following pretreatment with MTP-131 and incubation with H2O2, 5 µM freshly prepared H2DCFDA was added into cells and incubated at 37°C for 30 min in the dark. Following two rinses with PBS, cells were immediately imaged by confocal microscopy (ex/em: 495/525 nm).
Measurement of apoptosis
Apoptosis was measured using an Annexin V-FITC/PI apoptosis assay kit, according to the manufacturer's instructions. Briefly, cells were pretreated with 0.01, 0.1 or 1 µM MTP-131 for 1 h and then incubated with 500 µM H2O2 for 4, 8, 12 and 24 h. To quantify apoptosis by flow cytometry, cells were collected by trypsinization and centrifugation (100 × g for 5 min, at room temperature) at the appropriate time point. Following two rinses with PBS, cells were resuspended in 500 µl loading buffer and incubated with 5 µl Annexin V and 10 µl PI at room temperature for 5 min in the dark. Flow cytometry analysis was immediately performed (ex/em: 488/530 nm), using a FITC signal detector (FL1 channel) and PI emission signal detector (FL2 channel) to analyze Annexin V-FITC binding and PI staining, respectively. A total of 1×104 cells were collected and divided into four groups: viable cells (Annexin V-/PI-, Q3), early apoptotic cells (Annexin V+/PI-, Q4), late apoptotic cells (Annexin V+/PI+, Q2) or dead cells (Annexin V-/PI+, Q1). The apoptotic rate was calculated as the sum of the percentages of early apoptotic cells (Q4) and late apoptotic cells (Q2).
Measurement of cytochrome c release
The release of cytochrome c from mitochondria to the cytoplasm was measured by confocal microscopy as previously described (13). Briefly, cells were seeded onto Fisherbrand cover glass (Thermo Fisher Scientific, Inc.) at a density of 2,000 cells/chamber. Following pretreatment with 1 µM MTP-131 for 1 h, cells were incubated in the presence or absence of 500 µM H2O2 for 6 h at 37°C in 5% CO2. Freshly prepared MitoTracker (500 nm) was added to the cells and incubated for 30 min, just before the end of the 6 h incubation period. Cells were rinsed twice with PBS and fixed in 4% paraformaldehyde for 15 min, followed by 5 min permeabilization with methanol on ice. Following blocking with 5% bovine serum albumin (BSA, Jackson ImmunoResearch Europe Ltd. Suffolk, UK) for 30 min, cells were incubated with mouse monoclonal anti-cytochrome c antibody (sc-4280; 1:100, diluted with 1% BSA; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 4°C overnight. Following three rinses with PBS, cells were incubated with DyLight 488-conjugated goat anti-mouse immunoglobulin G antibody (85–11-4011-85; 1:500, diluted with 1% BSA; Multisciences Lianke Biotech Co., Ltd.) for 30 min at 37°C in the dark. Finally, cells were stained with Hoechst 33342 (1:1,000, Thermo Fisher Scientific, Inc.) for 5 min, washed and mounted with anti-fade fluorescence mounting medium (Applygen Technologies, Inc., Beijing, China). Images were visualized by confocal microscopy. Translocation of cytochrome c from mitochondria to cytoplasm was analyzed by overlapping staining of cytochrome c and MitoTracker. In each group, six slides were selected and five fields of view from each were assessed.
Analysis of cell morphology
RGC-5 cells were seeded at a density of 1×104 cells/well on a 6-well plate. Following pretreatment with 1 µM MTP-131 for 1 h and subsequent treatment with 500 µM H2O2 for 24 h, micrographs of all cultures were captured using a Zeiss LSM 510 Cell Observer system (Carl Zeiss, AG) to examine changes in cell morphology.
Statistical analysis
All assays were performed in at least three independent experiments and data are presented as the mean + standard error of the mean. Statistical analysis was performed by one-way analysis of variance and Newman-Keuls multiple comparison test, using GraphPad Prism 5.0 software (GraphPad Software Inc., La Jolla, CA, USA). P<0.05 was considered to indicate a statistically significant difference.
Results
MTP-131 treatment reduces H2O2-induced LDH release in RGC-5 cells
LDH assay was performed to assess cell viability in RGC-5 cells following H2O2-induced sustained oxidative stress. Incubation with 500 µM H2O2 for 24 h resulted in loss of cell viability compared with untreated cells, and the percentage of LDH release was 28.62±2.94% relative to the untreated cells (Fig. 1). Pretreatment with 1, 0.1 and 0.01 µM MTP-131 reduced LDH release in a dose-dependent manner compared to cells treated with H2O2 alone, with LDH release measured at 6.05±0.44, 13.08±0.53 and 22.39±1.73% relative to the untreated cells, respectively (P<0.01, P<0.01 and P<0.05, respectively, compared with H2O2 alone; Fig. 1).
MTP-131 treatment prevents H2O2-induced mitochondrial depolarization
RGC-5 cells were pretreated with 1 µM MTP-131 for 1 h, and then incubated with 500 µM H2O2 for 2 h. TMRM, a lipophilic cation which accumulates in mitochondria in a manner dependent on the membrane potential, was used to demonstrate ΔΨm in RGC-5 cells. As expected, untreated RGC-5 cells with healthy mitochondrial membrane potential exhibited high fluorescence intensity for TMRM (Fig. 2A, left panel), while incubation with 500 µM H2O2 alone for 2 h led to mitochondrial depolarization, resulting in loss of the TMRM dye from the mitochondria and a decrease in fluorescence intensity (Fig. 2A, middle panel). Pretreatment of the cells with 1 µM MTP-131 for 1 h, however, inhibited H2O2-induced mitochondria depolarization, as evidenced by the higher fluorescence intensity of TMRM in the MTP-131-treated cells (Fig. 2A, right panel) compared with the cells treated with H2O2 alone. Flow cytometry analysis confirmed that pretreatment with MTP-131 significantly prevented H2O2-induced mitochondria depolarization in a dose-dependent manner, compared with cells treated with H2O2 alone (Fig. 2B and C).
MTP-131 treatment inhibits H2O2-induced intracellular ROS production
RGC-5 cells were pretreated with 1 µM MTP-131 for 1 h, then incubated with 500 µM H2O2 for 24 h. Cells were then loaded with H2DCFDA for 30 min and DCF fluorescence was measured by flow cytometry and confocal microscopy. As demonstrated in Fig. 3A, incubation with H2O2 increased ROS production, as evidenced by the elevated DCF fluorescence intensity in the H2O2-treated cells (middle panel) compared with the untreated cells (left panel). Pretreatment with MTP-131, however, inhibited H2O2-induced ROS production, as evidenced by the lower DCF fluorescence intensity in MTP-131-treated cells (right panel; Fig. 3A) compared with the H2O2-treated cells (middle panel; Fig. 3A). Flow cytometry analysis further confirmed that pretreatment with MTP-131 reduced ROS generation in RGC-5 cells in a dose-dependent manner compared with cells treated with H2O2 alone (Fig. 3B and C).
MTP-131 treatment protects against H2O2-induced apoptosis
Apoptosis was measured by Annexin V-FITC/PI staining and flow cytometry analysis. RGC-5 cells were pretreated with MTP-131 for 1 h and then incubated with 500 µM H2O2 for 4, 8, 12 and 24 h. Following incubation with H2O2, the survival rate of RGC-5 cells decreased in a time-dependent manner compared with untreated cells (Fig. 4 and Table I). Pretreatment with 1 and 0.1 µM MTP-131 inhibited H2O2-induced apoptosis at 4, 8, 12 and 24 h compared with cells treated with H2O2 alone at the same time points (P<0.001 compared with H2O2 alone; Fig. 4 and Table I). Pretreatment with 0.01 µM MTP-131 also alleviated apoptosis at 4 and 8 h following H2O2 incubation, but no effect was observed at 12 and 24 h compared with cells treated with H2O2 alone (Fig. 4 and Table I).
MTP-131 treatment inhibits H2O2-induced release of cytochrome c from mitochondria to cytoplasm
The release of cytochrome c from mitochondria into the cytoplasm was examined by confocal microscopy. As demonstrated in Fig. 5, no release of cytochrome c (green fluorescence signal) from mitochondria (red fluorescence signal) was evident in untreated RGC-5 control cells (upper panels). Incubation with 500 µM H2O2 for 6 h, however, resulted in enhanced cytochrome c staining in the cytoplasm of RGC-5 cells (middle panels) compared with the untreated RGC-5 cells (Fig. 5), indicating that H2O2 induced the release of cytochrome c from mitochondria to cytoplasm. Pretreatment with 1 µM MTP-131 for 1 h inhibited the H2O2-induced release of cytochrome c, as evidenced by the decreased cytochrome c (green) fluorescent staining in MTP-131-treated cells (lower panels) compared with cells treated with H2O2 alone (Fig. 5).
MTP-131 treatment alleviates H2O2-induced morphological changes in RGC-5 cells
Morphological changes in RGC-5 cells were analyzed by phase contrast microscopy. As demonstrated in Fig. 6, normal untreated RGC-5 cells exhibit a neuron-like appearance, with thin axons extending from the cell bodies (left panel). However, following incubation with 500 µM H2O2 for 24 h, most cells exhibited a degenerative appearance, evidenced by the presence of vacuolar cell bodies and shrinkage of the axons (middle panel, Fig. 6) compared with the untreated cells. Pretreatment with 1 µM MTP-131 prevented the H2O2-induced morphological changes, with MTP-131-treated cells maintaining a relatively healthy appearance, with flat cell bodies and regular axon extensions (right panel, Fig. 6).
Discussion
In the present study, the antioxidant effect of MTP-131 against H2O2-induced sustained oxidative stress was examined in undifferentiated RGC-5 cells. The present findings demonstrated that MTP-131 treatment prevented mitochondrial depolarization, decreased intracellular ROS production, and inhibited apoptosis induced by sustained oxidative stress in RGC-5 cells.
H2O2 is widely used to induce oxidative stress in experimental studies, leading to an increase in intracellular accumulation of ROS and RGC apoptosis (14–16). In the present study, H2O2 (500 µM for 24 h) was used to induce a sustained oxidative stress, and was demonstrated to cause significant cell damage in RGC-5 cells, which is consistent with previous studies (14). MTP-131 pretreatment protected RGC-5 cells from mitochondrial membrane depolarization, reduced ROS production and inhibited cytochrome c release from mitochondria to cytoplasm. These effects were demonstrated to alleviate apoptosis in RGC-5 cells, thus suggesting that MTP-131 exhibited a potential protective effect against oxidative stress in RGC-5 cells through direct inhibition of mitochondria-mediated pathways.
RGC-5 cells have been widely used in in vitro experimental studies (17–19). However, the validity of this cell line is now questioned. It has been demonstrated that the RGC-5 cell line is of mouse origin, not rat as it was first described, and that it express photoreceptor markers instead of retinal ganglion markers (20,21). However, RGC-5 cells still have several properties in common with retinal progenitor cells and is therefore appropriate for neuronal cell studies (20). A series of retinal disorders have been related to oxidative damage, such as AMD, DR and RP (3,4,6). The retina is constantly exposed to visible light, which makes it extremely vulnerable to oxidative damage, due to its great energy demands and high oxygen consumption (5). A previous study have demonstrated that oxidative stress is extremely important in the retinal pigment epithelium (RPE) cells. ROS induces mitochondrial dysfunction in RPE, leading to RPE cell apoptosis and death of photoreceptor cells (22).
Compared with traditional antioxidants, MTP-131 represents the only known class of cell-permeable compounds that specifically concentrates in the inner mitochondrial membrane (7). With a 3+ net charge, MTP-131 selectively binds to cardiolipin and modulates its interaction with cytochrome c. By inhibiting the cytochrome c/cardiolipin complex peroxidase activity, MTP-131 optimizes mitochondrial electron transport and ATP synthesis (23,24). Numerous in vitro and animal studies have demonstrated the remarkable efficacy of MTP-131 in age-associated diseases. For example, daily treatment with MTP-131 reverses mitochondrial dysfunction and inhibits neuronal apoptosis and inflammation in mice with sepsis-associated encephalopathy (25). In addition, MTP-131 exhibits protective effects against cardiac ischemia-reperfusion injury (26). The effectiveness of cardiolipin combined with MTP-131 is currently being evaluated in a multinational clinical trial for reperfusion injury in patients with acute coronary events (ClinicalTrials.gov Identifier: NCT01572909), and a Phase 2 trial is underway assessing the effectiveness of MTP-131 on improving renal function following angioplasty for severe renal artery stenosis (ClinicalTrials.gov Identifier: NCT01755858) (27). Since MTP-131 has a unique mitochondria-targeted delivery, and exhibits potent antioxidant and neuroprotective effects both in vitro and in vivo, the present study aimed to evaluate the protective effect of MTP-131 against H2O2-induced oxidative damage in RGC-5 cells. The present findings demonstrated that MTP-131 inhibited H2O2-induced mitochondria depolarization, reduced intracellular ROS and prevented apoptosis in RGC-5 cells.
In conclusion, this novel class of targeted peptide therapeutics, including MTP-131, has the potential to restore mitochondrial bioenergetics. Further studies using animal models will be needed in order to fully explore this novel antioxidant approach for the treatment of age-related retinal diseases.
Acknowledgements
The authors would like to thank Stealth Peptides International Inc. for providing us with MTP-131 and a research grant. The present study was supported by the Zhejiang Provincial Natural Science Foundation of China (grant nos. LQ15H120001 and LY12H12008) and the National Natural Science Foundation of China (grant no. 81372930).
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