Open Access

Tetramethylpyrazine-mediated regulation of CXCR4 in retinoblastoma is sensitive to cell density

  • Authors:
    • Nandan Wu
    • Lijun Xu
    • Ying Yang
    • Na Yu
    • Zhang Zhang
    • Pei Chen
    • Jing Zhang
    • Mingjun Tang
    • Meng Yuan
    • Jian Ge
    • Keming Yu
    • Jing Zhuang
  • View Affiliations

  • Published online on: March 7, 2017     https://doi.org/10.3892/mmr.2017.6293
  • Pages: 2481-2488
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Retinoblastoma is the most common ocular tumor in children, and it causes extensive damage. Current treatment options for retinoblastoma include surgery, chemotherapy, radiotherapy and cryotherapy. However, the majority of chemotherapy medicines cause complications and side effects that lead to severe impairment of patient health. Previous studies have reported that tetramethylpyrazine (TMP), which is an extract of the Chinese herbal medicine Chuanxiong, reduces the risk of multidrug resistance in chemotherapy and inhibits the proliferation and metastasis of various types of cancer cells. However, the underlying molecular mechanism of TMP in retinoblastoma remains unclear. The current study demonstrated that C-X-C chemokine receptor type 4 (CXCR4) was expressed in WERI‑Rb1 cells and in retinoblastoma. Using reverse transcription‑quantitative polymerase chain reaction and western blotting techniques, the current study demonstrated that TMP significantly downregulated the expression of CXCR4 in WERI‑Rb1 cells cultured at high density, whereas it had a minor effect in low‑density WERI‑Rb1 cells; additionally, this effect occurred in a time‑dependent manner. TMP inhibited the proliferation of WERI‑Rb1 cells as effectively as a CXCR4 antagonist, AMD3100, consistent with a role of CXCR4 in cancer development. Notably, TMP did not affect the cell cycle of cells cultured at low density (1x105 cells/ml), whereas it induced G1‑phase arrest in high‑density cells (7.5x105 cells/ml; P<0.05). In addition, the expression of CXCR4 in primary rat retinal neurocytes was significantly downregulated by TMP treatment, and this treatment protected primary rat retinal neurocytes from H2O2‑induced damage. Thus, the results of this study indicate that TMP is a potential candidate for use in treatment of retinoblastoma, and also provides novel insights into the mechanisms of the anti‑cancer and neuroprotective effects of this extract.

Introduction

Retinoblastoma, a malignant intraocular tumor that develops in the retina, is one of the most common ocular diseases affecting children, particularly young children (<5 years of age) (1). This tumor may be monocular or binocular successively or simultaneously, and it can cause serious damage to patient vision, even resulting in blindness. Furthermore, if this cancer is not treated early it can cause local or distant metastasis. This advanced form of retinoblastoma can be life threatening. Currently, various treatment strategies have been proposed, including surgery, radiotherapy, chemotherapy or a combination of these treatments (1). Chemotherapy is the major conservative treatment; however, almost all types of chemotherapy drugs are associated with severe systemic complications. Therefore, identification of novel therapeutic medicines to supplement chemotherapy in retinoblastoma is of urgently required. The ideal drug should inhibit the proliferation of retinoblastoma cells and also exert protective activity on neurons.

In recent years, accumulating evidence has suggested that tetramethylpyrazine (TMP), an extract of the Chinese herbal medicine Chuanxiong, administered in combination with other treatments may significantly reduce the risk of multidrug resistance during chemotherapy (2,3) and inhibit the proliferation and metastasis of various types of cancer cells, including ovarian carcinoma, hepatocellular carcinoma, and lymphocytic leukemia cells (46). Vascular endothelial growth factor, hypoxia inducible factor-1α, the stromal cell-derived factor-1/C-X-C chemokine receptor type 4 (CXCR4) axis and P-glycoprotein may be involved in TMP-mediated bioactivity (79). Currently, the function of TMP in retinoblastoma remains unknown.

Additionally, Chuanxiong has been used for the clinical treatment of neural diseases in Chinese medicine practices for >2,000 years. Pharmacological studies have demonstrated that TMP improves microcirculation through its anti-platelet aggregation effects and arteriolar regulation. It also possesses anti-oxidant, anti-free radical injury and calcium antagonist effects. Thus, TMP has been used as a supplement to prevent and treat cerebral ischemia and degenerative diseases of the central nervous system, including Alzheimer's disease, Parkinson's disease and multiple sclerosis. More importantly, it has been demonstrated to have only mild side effects during clinical treatment (1013).

Furthermore, our previous study demonstrated that TMP protects primary rat brain neurocytes in vitro by markedly reducing the intracellular calcium level and inhibiting glutamate release via regulation of the expression of the chemokine receptor, CXCR4. It was also demonstrated that the TMP-mediated suppression of C6 glioma involves inhibition of CXCR4 expression (14). CXCR4 is a G-protein-coupled receptor with seven transmembrane-spanning domains most widely expressed in various types of cancer cells. It has been reported to mediate various processes that are essential for cancer progression, including tumor cell proliferation, metastasis, invasion and angiogenesis (1517). Notably, it was observed that TMP does not affect the cell cycle when C6 glioma cells are at 50–80% confluency. However, it can induce arrest in the S phase, significantly reducing the G1 and G2 populations of C6 glioma cells compared with controls, when cells are at 100% confluency (18).

Therefore, TMP may have a dual role in the inhibition of retinoblastoma growth and the protection of neurocytes. The present study was undertaken to examine whether TMP suppresses retinoblastoma cell growth by regulating CXCR4 expression and to determine whether its effect is associated with cell density.

Materials and methods

Patients

Retinoblastoma tissue was obtained from patients presenting at the Department of Pathology, Sun Yat-sen University (Guangzhou, China). The details and clinical demographics of patients are listed in Table I. This study was approved by the ethics committee of Sun Yat-sen University.

Table I.

Clinical demographics of 12 retinoblastoma patients.

Table I.

Clinical demographics of 12 retinoblastoma patients.

Patient numberAge at diagnosis (months)Sex (M/F)UL/BL
  148FUL
  224FUL
  312FUL
  436FUL
  548MUL
  625MBL
  720MUL
  828MUL
  911FUL
1036MUL
1114FUL
1226MBL

[i] M, male; F, female; UL, unilateral; BL, bilateral.

Reagents

The WERI-Rb1 human retinoblastoma cell line and HeLa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). Sprague Dawley (SD) rats were obtained from the animal center of Zhongshan Ophthalmic Center, Sun Yat-sen University. TMP, AMD3100, DMSO and propidium iodide (PI) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Rabbit anti-CXCR4 and GAPDH, mouse anti-microtubule associated protein-2 (MAP-2) primary polyclonal antibodies were purchased from Abcam (Cambridge, UK; cat. no. ab2047), ProteinTech Group, Inc. (Chicago, IL, USA; cat. no. 10494-1-AP), and Boster Biological Technology, Ltd. (Wuhan, China; cat. no. BM1243), respectively. Horseradish peroxidase (HRP)-conjugated anti-rabbit IgG, Alexa Fluor 555 anti-rabbit IgG and Alexa Fluor 488 anti-mouse IgG were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA; cat. nos. 7074, 4413 and 4408, respectively). TRIzol Reagent and all of the cell culture media/reagents and salt solutions were obtained from Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA). A SYBR PrimeScript™ RT-PCR kit was purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Amersham ECL reagents for western blotting were obtained from GE Healthcare Bio-Sciences (Pittsburgh, PA, USA).

Cell culture

WERI-Rb1 and HeLa cells were cultured in RPMI-1640 medium and Dulbecco's modified Eagle's medium (DMEM), respectively, supplemented with 10% fetal bovine serum, 100 U/ml penicillin and 100 mg/ml streptomycin in a humidified atmosphere of 5% carbon dioxide at 37°C. For primary retinal neurocyte culture, the protocol was as follows: Briefly, P1-day-old SD rats (n=9) were sacrificed by an intraperitoneal injection of Nembutal (60 mg/kg; Sigma-Aldrich). The retinas that were separated from the enucleated eyeballs were incubated for 20 min at 37°C in a solution containing 0.125% trypsin, to dissociate cells. To yield a suspension of single cells, the tissue was then triturated sequentially through a narrow-bore Pasteur pipette in a solution of DMEM supplemented with 10% fetal bovine serum. Cells were plated at a density of ~1×106 cells/ml on a culture plate pre-coated with 0.01% poly-L-lysine and cultured in DMEM supplemented with 10% fetal bovine serum, in a humidified atmosphere of 5% carbon dioxide at 37°C for 2 h. After 12 h, the cells were treated with 10 µM Ara-C (Sigma-Aldrich) to suppress the growth of non-neurocytes. TMP was dissolved in component solvent (DMSO: saline, 1:1) and AMD3100 was dissolved in saline to the appropriate concentrations. The component solvent and saline were applied as vehicle controls.

Cell viability assays using cell counting Kit-8 (CCK-8)

Cell viability was measured using a CCK-8 (Dojindo Molecular Technologies, Inc., Kumamoto, Japan). Exponentially growing WERI-Rb1 cells (1×105 or 7.5×105 cells/ml) were seeded in a 96-well plate in 100 µl complete medium for 4 h at 37°C prior to addition of 200 µM TMP, 10 µg/ml AMD3100 or a vehicle control. After 24 h of treatment, 10 µl CCK-8 reagent was added to each well and incubated at 37°C for 1 h. Subsequently, absorbance was measured at 450 nm using a fluorescence plate reader (Power Wave XS; BioTek China, Beijing, China). Cell viability was determined by the optical density ratio of treated cells over the untreated control.

Cell cycle assay

WERI-Rb1 cells were plated at a high cell density (7.5×105 cells/ml) or low cell density (1×105 cells/ml) and treated with TMP (200 µM) or a vehicle control. Cells were collected after 24 h of treatment and washed with ice-cold PBS and then fixed with 75% ice-cold ethanol at 4°C overnight. Before analysis, the cells were washed twice with PBS and incubated in a PI staining solution (0.05 mg/ml PI, 1 mM EDTA, 0.1% Triton-X-100™ and 1 mg/ml RNase A) for 30 min at 37°C. Subsequently, fluorescent cells were analyzed using a BD FACSort™ flow cytometer (BD Biosciences, San Jose, CA, USA). The data were analyzed with Flow Jo software (Tree Star, Inc., Ashland, OR, USA).

Reverse transcription-polymerase chain reaction (RT-PCR) assay

Total RNA was isolated with TRIzol Reagent and dissolved in RNase-free water. Total RNA (1 µg) was subjected to reverse transcription using a PrimeScript™ RT Reagent kit (Takara Biotechnology Co., Ltd., Dalian, China) following the manufacturer's protocol.

Semi-quantitative PCR was performed to measure the expression of CXCR4 in WERI-Rb1 cells and HeLa cells under normal growth conditions using an automated thermocycler (Biometra GmbH, Göttingen, Germany). The PCR program was as follows: Pre-denaturation at 94°C for 5 min; and 30 cycles of denaturation at 94°C for 1 min, annealing at 60°C, and extension at 72°C for 1 min. PCR products were separated by 2% agarose gel electrophoresis, and the band intensities on the resulting gels were determined by Scion Image software (Scion Image Corporation, Fredrick, MD, USA). β-actin gene expression was examined as an internal control. Quantitative PCR was employed to compare the expression of CXCR4 in WERI-Rb1 cells treated with TMP (200 µM) or a vehicle control using the SYBR Green system (Takara Biotechnology Co., Ltd.), using the aforementioned thermocycling conditions. The quantity of target gene mRNA relative to that of the internal control gene, β-actin, was calculated using the 2−ΔΔCT method (19). The following primer pairs were used: CXCR4, 5′-CTTATCCTGCCTGGTATTGTC-3′ and 5′-CAATGTAGTAAGGCAGCCAAC-3′; and for β-actin, 5′-CACCACACCTTCTACAATGAG-3′ and 5′-TAGCACAGCCTGGATAGCAAC-3′. The data were analyzed in triplicate.

Western blot assay

WERI-Rb1 cells were seeded in 60 mm dishes at different cell densities (1×105 or 7.5×105 cells/ml) and treated with TMP (200 µM) or a vehicle control for varying durations (12 or 24 h). After treatment, the cells were washed with PBS and collected in radioimmunoprecipitation assay lysis buffer (Beyotime Institute of Biotechnology, Jiangsu, China). According to the bicinchoninic acid method for protein quantification, equal amounts of protein (30 µg/well) were separated on an 8% SDS-polyacrylamide gel by electrophoresis and electrophoretically transferred to a PVDF membrane (EMD Millipore, Billerica, MA, USA) at 250 mA for 1.5 h. The membrane was blocked with a solution of 5% dried fat-free milk in TBS-Tween (TBS containing 0.1% Tween-20) for 2 h, and then membrane-bound proteins were probed with primary antibodies against CXCR4 (1:500 dilution) and GAPDH (1:1,000 dilution) at 4°C overnight. Subsequently, the membrane was washed 3 times with TBST for 5 min each time, followed by incubation with HRP-conjugated anti-rabbit IgG (1:10,000) for 1 h at room temperature. GAPDH served as an internal control. Finally, protein bands were detected with enhanced chemiluminescence (EMD Millipore). Densitometric analysis of the bands compared with the density of CXCR4 and GAPDH was performed using ImageJ software (imagej.nih.gov).

Immunohistofluorescence assay

Human retinoblastoma tissues were originally fixed with 10% formalin and then embedded in optimal cutting temperature compound. Retinoblastoma tissue sections of 6 µm were used. Human retinoblastoma tissue, untreated WERI-Rb1 cells and primary cultured neurocytes were fixed with ice-cold 100% methanol for 15 min and then blocked with 10% normal goat serum (Cell Signaling Technology, Inc., Danvers, MA, USA) for 30 min. Subsequently, the human retinoblastoma tissue and untreated WERI-Rb1 cells were incubated overnight at 4°C with primary antibody against CXCR4 (1:500 dilution), and primary cultured neurocytes were incubated with primary antibodies against CXCR4 (1:500 dilution) and MAP-2 (1:100 dilution). Alexa Fluor 555 anti-rabbit lgG and Alexa Fluor 488 anti-mouse lgG were used as secondary antibodies (1:500 dilution) for a further 1 h at room temperature, and nuclei were stained with DAPI.

H2O2-induced damage and MTT viability assays

Primary cultured rat retinal neurons were seeded in a 24-well plate in 500 µl complete medium and then treated with TMP (200 µM) or a vehicle control for 48 h at 37°C. After the TMP treatment, the cells were treated with H2O2 (600 µM) for 15 min at 37°C. Subsequently, they were incubated in H2O2-free medium with TMP or a vehicle control at 37°C for 2.5 h. After 0, 24 or 48 h, 50 µl MTT was add to each well, the plate was incubated for 4 h at 37°C and 500 µl DMSO was added to each well. Absorbance was measured at 490 nm using a fluorescence plate reader (Power Wave XS). Cell viability was determined according to the optical density ratio of a treated culture over an untreated control.

Statistical analysis

All experiments were performed at least three times in vitro. Data are expressed as the mean ± standard error. Differences between mean values were evaluated with two-tailed Student's t-test (for 2 groups). All calculations and statistical tests were performed using Excel 2003 (Microsoft Corporation, Redmond, WA, USA). P<0.05 was considered to indicate a statistically significant difference.

Results

CXCR4 is expressed in WERI-Rb1 cells and retinoblastoma tissue

As CXCR4 has important roles in cancer development and progression (15), CXCR4 expression in WERI-Rb1 cells and in HeLa cells was determined as a positive control. Total RNA and whole-cell lysates were obtained for semi-quantitative PCR and western blot analyses. As demonstrated in Fig. 1A and B, CXCR4 mRNA and protein expression was higher in WERI-Rb1 cells compared with HeLa cells. Immunofluorescence staining confirmed the expression of CXCR4 in the cytoplasm of WERI-Rb1 cells and retinoblastoma tissue (Fig. 1C). Therefore, it may be a potential therapeutic target for retinoblastoma treatment.

TMP-mediated downregulation of CXCR4 in WERI-Rb1 cells is dependent on cell density

It was previously reported that TMP suppresses the growth of C6 glioma by reducing the expression of CXCR4 (14). To examine whether TMP downregulates CXCR4 expression in WERI-Rb1 cells and to determine whether its effect is associated with cell density, we measured the mRNA and protein levels of CXCR4 after TMP treatment (12 or 24 h) in WERI-Rb1 cells plated at a low density (1×105 cells/ml) or high density (7.5×105 cells/ml). Fig. 2A demonstrated that the mRNA expression of CXCR4 in WERI-Rb1 cells was not altered after TMP treatment for 12 h at the low density; no significant difference was observed between the test and control groups. At 24 h, CXCR4 expression was marginally downregulated by the TMP treatment, exhibiting a 12.8% decrease (P<0.05). However, CXCR4 mRNA expression was significantly decreased after TMP treatment for 12 and 24 h at the high cell density, exhibiting a 28.9 and 37.5% decrease, respectively (P<0.05; Fig. 2A). Moreover, the decrease in CXCR4 mRNA expression at the high cell density was 2.93-fold greater than that at the low cell density at the 24 h time point (P<0.05; Fig. 2). The results of western blotting were consistent with those of RT-qPCR (Fig. 2B). Relative quantification of CXCR4 expression revealed that there was also no significant difference between the test and control groups after TMP treatment at the 12 h time point at the low density, with a 14.3% decrease in CXCR4 expression at the 24 h time point (P<0.05; Fig. 2C). Similarly, its expression was markedly decreased after TMP treatment for 12 and 24 h at the high cell density, exhibiting a 22.9 and 38.7% decrease, respectively (P<0.05; Fig. 2C), and the decrease in its expression at the high cell density was 2.76-fold greater than that at the low cell density at 24 h time point (P<0.05; Fig. 2C). These findings further confirm that TMP significantly inhibits CXCR4 expression in WERI-Rb1 cells and that its effect is sensitive to cell density. Furthermore, TMP treatment downregulated CXCR4 expression in WERI-Rb1 cells in a time-dependent manner (Fig. 2). These results further support the hypothesis that CXCR4 may be involved in mediating the effect of TMP on retinoblastoma and that TMP-mediated downregulation of CXCR4 may be associated with cell density.

Effects of TMP on inhibiting proliferation and the cell cycle of WERI-Rb1 cells are dependent on cell density

Considering that the TMP-mediated downregulation of CXCR4 in WERI-Rb1 cells depends on cell density and that CXCR4 expression is closely associated with to the proliferation of cancer cells, WERI-Rb1 cells were seeded at different cell densities (105 or 7.5×105 cells/ml) to determine the bioactivity of TMP on WERI-Rb1 cell growth and whether the inhibitory effect is associated with cell density. AMD 3100, a CXCR4 antagonist, was used as a positive control. As presented in Fig. 3A, TMP (200 µM) reduced WERI-Rb1 cell viability at after 24 h of treatment, and this inhibition was stronger at the high cell density than at the low cell density, with inhibition rates of 10 and 27%, respectively (P<0.05). AMD3100-treated WERI-Rb1 cells also exhibited reduced viability in a density-dependent manner, with inhibition rates of 8 and 24% at the low cell density and at the high cell density, respectively (P<0.05; Fig. 3B). The inhibition of cell growth may be a result of cell cycle arrest. To determine whether the inhibitory effects of TMP on WERI-Rb1 cell proliferation at different cell densities involves cell cycle alterations, the cell cycle profiles of WERI-Rb1 cells at different cell densities were examined by flow cytometry. The cell cycle was analyzed after treatment with TMP for 24 h, and the results demonstrated that at a low cell density (1×105 cells/ml), there were no significant differences in the cell cycle profiles of WERI-Rb1 cells between the test and control groups (Fig. 3C). However, TMP treatment was capable of inducing G1 phase arrest (62.83±7.53%) in the high-density cells (7.5×105 cells/ml) compared with control cells (55.52±6.86%; P<0.05; Fig. 3D). Therefore, the results demonstrated that the inhibition of WERI-Rb1 cell proliferation by TMP was greater at the high cell density than at the low density. Furthermore, its inhibitory effect on cell proliferation was induced by other factors besides the arrest of the cell cycle at the G1 phase and cell cycle inhibition.

TMP downregulates the expression of CXCR4 in primary rat retinal neurocytes and protects these cells from H2O2-induced damage

Retinoblastoma is a malignant intraocular tumor that arises in the retina, and development of this type of tumor can cause injury to retinal neurons. Our previous studies have verified that TMP protects rat cerebral neurons by downregulating CXCR4 expression (14). To confirm that this extract has protective effects on retinal neurons, the expression of CXCR4 in primary rat retinal neurocytes was measured in vitro. Dual immunofluorescence staining for MAP-2 and CXCR4 revealed that CXCR4 was expressed in retinal neurons. MAP-2 was used as a marker of neuronal cells (Fig. 4A). After TMP treatment for 24 h, RT-qPCR was performed to determine CXCR4 mRNA expression in primary retinal neurocytes. As demonstrated in Fig. 4B, TMP significantly inhibited CXCR4 mRNA expression in these cells compared with the control cells (P<0.05), with an inhibition rate of 42.9%. Subsequently, retinal neurocytes were pretreated with TMP or a vehicle control for 48 h and then the cells were exposed to H2O2 (600 µM) for 15 min. Subsequently, the cells were incubated in an H2O2-free medium with TMP or a vehicle control at 37°C. After 0, 24 or 48 h, an MTT assay was performed to measure cell viability. As demonstrated in Fig. 4C, neurons in the experimental group exhibited increased cell viability compared with the control group, with a viable cell rate increase to 120% (24 h) or 126% (48 h) relative to the control (P<0.05); at 0 h no significant difference was observed between the two groups. Additionally, the effect occurred in a time-dependent manner. Therefore, the results indicated that TMP possesses an anti-retinoblastoma function and exerts a protective effect on neurons.

Discussion

A growing body of evidence suggests that TMP possesses a potent inhibitory effect on cancer, and the relevant molecular mechanisms involved have been reported to include decreased DNA synthesis in cells via inhibition of mitosis and reduced of the expression of resistant genes (2). The present study demonstrated that TMP downregulated the expression of CXCR4 at the RNA and protein levels in WERI-Rb1 cells, and that it reduced WERI-Rb1 cell viability. Notably, the effect of TMP on WERI-Rb1 cells was sensitive to cell density. At a high cell density, the expression of CXCR4 was reduced dramatically, and cell viability was strongly inhibited by TMP treatment. However, the effect of TMP was minimal at a low cell density. In addition, TMP downregulated the expression of CXCR4 in rat retinal neurocytes and protected them from H2O2-induced damage in vitro.

Previous studies have indicated that CXCR4 has important roles in tumor progression (1517). For example, treatment with AMD3100 (a CXCR4 antagonist) or chemotherapy combined with AMD3100 has been shown to induce caspase-3-mediated apoptosis and to inhibit glioma growth in vivo (20). A high level of CXCR4 expression promotes tumor proliferation, angiogenesis, migration and metastasis (21). It has been demonstrated that the expression of CXCR4 in WERI-Rb1 cells was also dependent on cell density, as expression in high-density cells was higher than that in low-density cells (unpublished data). Notably, TMP significantly downregulated CXCR4 expression in high-density WERI-Rb1 cells, however the effect was not as potent in cells cultured at low density. Based on these evidences, we hypothesize that TMP possesses a strong anti-retinoblastoma effect when a tumor is actively proliferating, thus may be of therapeutic value to supplement chemotherapy to inhibit tumor growth and metastasis. Elucidation of the mechanism of the TMP-mediated downregulation of CXCR4 in high-density cells requires further investigation.

CXCR4 is closely associated with the cell cycle (22,23), and its downregulation results in reductions in the expression of certain cell cycle-associated proteins, including cyclin D1, which is a subtype of cyclin D that affects the G1/S phase control point in the cell cycle (24,25). Accordingly, the cell cycle profile data in the current study demonstrated that TMP treatment resulted in arrest of WERI-Rb1 cells in the G1 phase when the cells were cultured to a high density. These cell cycle data are similar to the results of our previous study (18), revealing that TMP only affects the cell cycle of glioma C6 cells at 100% confluency. However, the different cell type the previous study produced differing results, with TMP inducing arrest in the S phase in C6 cells, and significantly reducing the G1 and G2 populations compared with control cells (18). Therefore, TMP may reduce CXCR4 expression, and subsequently arrest the cell cycle at the G1 phase, eventually inhibiting tumor cell proliferation in retinoblastoma. Additionally, in the current study, the cell cycle of WERI-Rb1 cells was not altered after TMP treatment for 24 h at a low cell density, with only a minor inhibitory effect on cell proliferation. These data suggest that the cell cycle inhibition may a factor that affect retinoblastoma cell proliferation.

Furthermore, Chuanxiong has been used in the clinical setting >2,000 years, and its bioactive extract, TMP, is also an effective medicine currently used in the treatment of neural diseases in China (1013). Our previous study has demonstrated that CXCR4 is a target gene of TMP in neural protection (14). The results of the present study demonstrated that CXCR4 was expressed in retinal MAP-2-positive cells in vitro, and TMP significantly downregulated CXCR4 expression in retinal neurocytes and increased cell viability. Therefore, TMP not only inhibits retinoblastoma cell growth, it also protects retinal neurocytes.

In conclusion, the findings of the present study suggest that the TMP-mediated CXCR4 pathway may be sensitive to cell density. TMP could be a potentially effective and safe therapeutic agent to supplement chemotherapy during retinoblastoma treatment. The current study will extend the application of TMP treatment in clinical therapy.

Acknowledgements

This study was supported by grants from the National Natural Science Foundation (project no. 81370987).

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May-2017
Volume 15 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wu N, Xu L, Yang Y, Yu N, Zhang Z, Chen P, Zhang J, Tang M, Yuan M, Ge J, Ge J, et al: Tetramethylpyrazine-mediated regulation of CXCR4 in retinoblastoma is sensitive to cell density. Mol Med Rep 15: 2481-2488, 2017.
APA
Wu, N., Xu, L., Yang, Y., Yu, N., Zhang, Z., Chen, P. ... Zhuang, J. (2017). Tetramethylpyrazine-mediated regulation of CXCR4 in retinoblastoma is sensitive to cell density. Molecular Medicine Reports, 15, 2481-2488. https://doi.org/10.3892/mmr.2017.6293
MLA
Wu, N., Xu, L., Yang, Y., Yu, N., Zhang, Z., Chen, P., Zhang, J., Tang, M., Yuan, M., Ge, J., Yu, K., Zhuang, J."Tetramethylpyrazine-mediated regulation of CXCR4 in retinoblastoma is sensitive to cell density". Molecular Medicine Reports 15.5 (2017): 2481-2488.
Chicago
Wu, N., Xu, L., Yang, Y., Yu, N., Zhang, Z., Chen, P., Zhang, J., Tang, M., Yuan, M., Ge, J., Yu, K., Zhuang, J."Tetramethylpyrazine-mediated regulation of CXCR4 in retinoblastoma is sensitive to cell density". Molecular Medicine Reports 15, no. 5 (2017): 2481-2488. https://doi.org/10.3892/mmr.2017.6293