MicroRNA‑18b acts as an oncogene in gastric cancer by directly targeting Kruppel‑like factor 6

Retraction in: /10.3892/mmr.2022.12916

  • Authors:
    • Dongming Luo
    • Junqiang Chen
    • Shifeng Huang
    • Junyi Xu
    • Xuemin Song
    • Pengcheng Yu
  • View Affiliations

  • Published online on: January 8, 2019     https://doi.org/10.3892/mmr.2019.9830
  • Pages: 1926-1934
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Abstract

Gastric cancer (GC) is the fourth most frequently occurring cancer and the second most common cause of cancer‑associated mortality worldwide. An increasing number of studies have reported that microRNAs (miRNAs/miRs) contribute to the regulation of GC development and progression. Therefore, investigation of the miRNAs involved in the development of GC may result in identification of an effective therapeutic target for patients with this malignancy. miR‑18b has been reported to be aberrantly expressed in several types of human cancer. However, the expression pattern, biological role and specific functional mechanism of miR‑18b in GC remains to be elucidated. In the present study, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis revealed that miR‑18b was significantly upregulated in GC tissues and cell lines compared with normal gastric tissues and the human gastric epithelial immortalized cell line GES‑1, respectively. High miR‑18b expression was significantly associated with lymph node metastasis, invasive depth and the Tumor Node Metastasis stage of patients with GC. Additionally, functional assays indicated that the inhibition of miR‑18b attenuated cell proliferation and invasion in GC. Furthermore, Kruppel‑like factor (KLF)‑6 was identified as a direct target gene of miR‑18b in GC, from the results of bioinformatics analysis, a luciferase reporter assay, RT‑qPCR and western blot analysis. An inverse association was observed between miR‑18b and KLF6 mRNA levels in GC tissues. KLF6 knockdown partially abrogated the effects of miR‑18b inhibition on GC cell proliferation and invasion. Therefore, miR‑18b/KLF6 targeted therapy may provide a promising treatment for patients with GC.

Introduction

Gastric cancer (GC) is the fourth most frequently occurring cancer and the second most common cause of cancer-associated mortality worldwide (1), with approximately 1,000,000 newly diagnosed cases and >700,000 mortalities due to GC estimated to occur annually worldwide. To date, several risk factors influencing the formation and progression of GC have been identified, including Helicobacter pylori infection, smoking, obesity, dietary habits and chronic atrophic gastritis (2,3). Despite significant progress in treatment development, the clinical outcome of patients with advanced-stage GC remains poor, with a 5-year survival rate of only 30–50% (4). Tumor formation and development in GC is multifactorial and significant genetic and epigenetic changes have been demonstrated to contribute to GC (5). However, the molecular mechanism associated with GC occurrence and development remains largely unknown. Therefore, further insight into the mechanisms that regulate GC progression may be advantageous in the discovery and development of novel therapies for patients with this disease.

MicroRNAs (miRNAs/miRs) are a group of short non-coding RNA molecules that are critical regulators of oncogenesis and cancer progression (6). miRNAs silence the expression of their target genes through direct binding to the 3′untranslated regions (UTRs), resulting in translation inhibition and/or mRNA degradation (7). Previous studies have reported that miRNAs modulate the expression of >50% of human protein-coding genes involved in the regulation of a wide range of physiological and pathological processes, including cell proliferation, cycle, apoptosis, differentiation, metabolism and tumorigenesis (8). Several miRNAs have been demonstrated to be aberrantly expressed in the majority of human malignancies (911). Abnormally expressed miRNAs may result in the progression or inhibition of normal cell growth patterns, thus leading to cancer initiation and progression (12). miRNAs may have roles in tumor suppression or oncogenesis, depending on the functional roles of their target genes (13,14). Hence, investigation of the regulatory roles of miRNAs in human cancers may provide promising therapeutic targets for antitumor therapy.

The aberrant expression of miR-18b has been widely reported to occur in several types of human cancer (1517). However, the expression pattern, biological role and specific functional mechanism of miR-18b in GC remains to be fully elucidated. A better understanding of miR-18b in GC may provide novel therapeutic targets for the treatment of patients with GC.

Materials and methods

Human tissue samples

The present study was approved by the Ethics Committee of The First Affiliated Hospital of Guangxi Medical University (Nanning, China). Written informed consent was obtained from all participants. A total of 49 GC and matched adjacent normal gastric tissue samples were collected from patients (28 males, 21 females; age range, 52–79 years) who underwent surgery resection at The First Affiliated Hospital of Guangxi Medical University between July 2014 and November 2016. No patients received chemotherapy, radiotherapy or other treatments prior to surgery. Upon resection, all tissues were immediately frozen in liquid nitrogen and subsequently stored at −80°C.

Cell culture and transfection

GC cell lines (AGS, SGC-7901, BGC-823 and MGC-803) and the human gastric epithelial immortalized GES-1 cell line were purchased from American Type Culture Collection (Manassas, VA, USA). All cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin solution at 37°C in a humidified incubator with 5% CO2. DMEM, FBS and antibiotic solution were all purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA).

miR-18b inhibitor, miRNA inhibitor negative control (NC inhibitor), KLF6-specific small interfering RNA (siRNA) and negative control siRNA (NC siRNA) were synthesized by Shanghai GenePharma Co., Ltd., (Shanghai, China). The miR-18b inhibitor sequence was 5′-CUAACUGCACUAGAUGCACCUUA-3′ and the NC inhibitor sequence was 5′-CAGUACUUUUGUGUAGUACAAA-3′.

The KLF6 siRNA sequence was 5′-GCAGGAAAGUUUACACCAATT-3′ and the NC siRNA sequence was 5′-UUCUCCGAACGUGUCACGUTT-3′. Cells were plated into 6-well plates at a density of 8×105 cells/well. Following incubation overnight, cells were transfected with miR-18b inhibitor (100 pmol), NC inhibitor (100 pmol), KLF6 siRNA (100 pmol) or NC siRNA (100 pmol) using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. A total of 48 h following transfection, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to determine miR-18b expression. Cell Counting kit-8 (CCK8) and cell invasion assays were performed at 24 and 48 h post transfection. Western blot analysis was carried out at 72 h following transfection.

RT-qPCR

Total tissue or cell RNA was isolated using TRIzol® reagent (Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol. To quantify miR-18b expression, total RNA was converted into first-strand complementary DNA (cDNA) using a TaqMan microRNA reverse transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.). The temperature protocol for reverse transcription was as follows: 16°C for 30 min, 42°C for 30 min and 85°C for 5 min. Subsequently, PCR amplification was performed to detect miR-18b expression with the TaqMan microRNA PCR kit (Applied Biosystems; Thermo Fisher Scientific, Inc.). The cycling conditions were as follows: 50°C for 2 min, 95°C for 10 min; 40 cycles of denaturation at 95°C for 15 sec; and annealing/extension at 60°C for 60 sec. To quantify KLF6 mRNA expression, cDNA was synthesized from total RNA using a Moloney Murine Leukemia Virus reverse transcriptase kit (Promega Corporation, Madison, WI, USA). The temperature protocol for reverse transcription was as follows: 95°C for 2 min; 20 cycles of 94°C for 1 min, 55°C for 1 min and 72°C for 2 min; and 72°C for 5 min. cDNA was subsequently subjected to qPCR using SYBR® Premix Ex TaqTM II (Takara Biotechnology Co., Ltd., Dalian, China). The cycling conditions were as follows: 5 min at 95°C, followed by 40 cycles of 95°C for 30 sec and 65°C for 45 sec. U6 snRNA and GAPDH were used as internal reference for miR-18b and KLF6 mRNA, respectively. The primers were designed as follows: miR-18b, 5′-GGGTAAGGTGCATCTAGTGC-3′ (forward) and 5′-CAGTGCGTGTCGTGGAGT-3′ (reverse); U6, 5′-GCTTCGGCAGCACATATACTAAAAT-3′ (forward) and 5′-CGCTTCACGAATTTGCGTGTCAT-3′ (reverse); KLF6, 5′-CGGACGCACACAGGAGAAAA-3′ (forward) and 5′-CGGTGTGCTTTCGGAAGTG-3′ (reverse); and GAPDH, 5′-CGGAGTCAACGGATTTGGTCGTAT-3′ (forward) and 5′-AGCCTTCTCCATGGTGGTGAAGAC-3′ (reverse). Data were analyzed using the 2−ΔΔCq method (18).

Cell Counting kit-8 (CCK8) assay

A CCK8 assay was performed to detect cell proliferation. Cells were plated into 96-well plates at a density of 3×103 cells/well. Following transfection, cells were incubated at 37°C in a humidified incubator with 5% CO2 for 0, 24, 48 and 72 h. At each time point, the CCK8 assay was conducted according to the manufacturer's protocol. Briefly, 10 µl CCK8 solution (Dojindo Molecular Technologies, Inc., Kumamoto, Japan) was added to each well and incubated at 37°C with 5% CO2 for further 2 h. Optical density values were measured at a wavelength of 450 nm using a microplate spectrophotometer (Bio-Tek Instruments, Inc., Winooski, VT, USA).

Cell invasion assay

Matrigel coated Transwell chambers (pore size, 8-µm; BD Biosciences, Franklin Lakes, NJ, USA) were used to examine cell invasive ability. Transfected cells were collected 48 h post-transfection. A total of 1×105 cells in 200 µl FBS-free DMEM medium were added to the upper chamber. The lower chambers were filled with 500 µl DMEM supplemented with 10% FBS. Following incubation for 24 h, cells remaining on the upper surface were removed using cotton swabs. The invaded cells were fixed with 100% methanol at room temperature for 15 min, stained with 0.1% crystal violet at room temperature for 15 min and washed with PBS. The number of invasive cells was counted under an inverted light microscope (Olympus Corporation, Tokyo, Japan) in five randomly selected fields.

Bioinformatics analysis and luciferase reporter assay

The putative target genes of miR-18b were predicted using miRanda (August 2010 Release, Last Update: 2010-11-01; www.microrna.org) and TargetScan (version 7.1; www.targetscan.org). KLF6 was predicted as a potential target of miR-18b. A luciferase reporter assay was utilized to investigate if KLF6 is a direct target of miR-18b. Luciferase plasmids pmirGLO-KLF6-3′-UTR wild type (Wt) and pmirGLO-KLF6-3′-UTR mutant (Mut), were chemically synthesized by Shanghai GenePharma Co., Ltd. Cells were seeded in 24-well plates at an initial density of 1.5×105 cells/well. Following an overnight incubation, cells were transfected with miR-18b inhibitor or NC inhibitor, in addition to pmirGLO-KLF6-3′-UTR Wt or pmirGLO-KLF6-3′-UTR Mut using Lipofectamine® 2000. Following incubation at 37°C with 5% CO2 for 48 h, cells were harvested and the luciferase activity was detected using the Dual-Luciferase Reporter assay system (Promega Corporation) according to the manufacturer's protocol. Firefly luciferase activity was normalized Renilla luciferase activity.

Western blot analysis

The primary antibodies used in the present study were mouse anti-human KLF6 primary antibody (1:1,000; cat. no. sc-134374) and mouse anti-human GAPDH primary antibody (1:1,000; cat. no. sc-32233), purchased from Santa Cruz Biotechnology, Inc., Dallas, TX, USA. Total protein was extracted from tissues or cells using a radioimmunoprecipitation assay lysis buffer (Beyotime Institute of Biotechnology, Haimen, China). The concentration of total protein was quantified with a bicinchoninic acid protein assay kit (Beyotime Institute of Biotechnology). Equal amounts of protein (30 µg) were loaded into each lane and separated by 10% SDS-PAGE, followed by transfer onto polyvinylidene difluoride membranes. Membranes were blocked with 5% skimmed milk in Tris-buffered saline containing 0.1% Tween-20 (TBST) at room temperature for 1 h, followed by incubation with primary antibodies at 4°C overnight. Membranes were subsequently washed with TBST three times and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary IgG goat anti-mouse (1:5,000; cat. no. sc-2005; Santa Cruz Biotechnology, Inc.) at room temperature for 2 h. Protein bands were visualized using an enhanced chemiluminescence plus reagent (GE Healthcare Life Sciences, Little Chalfont, UK). Protein expression was quantified using Quantity One software version 4.62 (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

Statistical analysis

Data is expressed as the mean ± standard deviation of at least 3 independent experiments and was analyzed using the Student's t-test or one-way analysis of variance followed by the Student-Newman-Keuls multiple comparisons test. The association between miR-18b and clinicopathological characteristics of patients with GC was analyzed using a univariate χ2 test. Spearman's correlation analysis was performed to evaluate the association between miR-18b and KLF6 mRNA levels in GC tissues. SPSS software, version 13.0 (SPSS, Inc., Chicago, IL, USA) was used for statistical analysis. P<0.05 was considered to indicate a statistically significant difference.

Results

MiR-18b is upregulated in GC tissues and cell lines

miR-18b expression was detected in 49 GC and matched adjacent normal gastric tissue samples. RT-qPCR analysis revealed that miR-18b expression was significantly upregulated in GC tissues compared with the adjacent normal gastric tissues (P<0.05; Fig. 1A). Additionally, the expression levels of miR-18b in GC cell lines and the human gastric epithelial immortalized cell line GES-1 were also determined using RT-qPCR. Compared with GES-1, higher expression levels of miR-18b were detected in all four GC cell lines (P<0.05; Fig. 1B). As AGS and BGC-823 cells expressed relatively higher miR-18b expression amongst the four GC cell lines, these two cell lines were selected for the subsequent experiments. These results indicated that miR-18b is upregulated in both GC tissues and cell lines.

Association between miR-18b and clinicopathological factors of patients with GC

To explore the clinical value of miR-18b in GC, patients were divided into miR-18b high-expression group (n=25) and low-expression groups (n=24) based on the median expression of miR-18b (1.86). As presented in Table I, high miR-18b expression was associated with lymph node metastasis (P=0.032), invasive depth (P=0.015) and Tumor Node Metastasis (TNM) stage (P=0.015) in patients with GC. However, no significant difference was observed between miR-18b expression and other clinicopathological factors, including age, sex, tumor size and differentiation (P>0.05). These results suggested that miR-18b may be associated with the malignant progression of GC.

Table I.

Association between miR-18b expression and clinicopathological features of patients with gastric cancer.

Table I.

Association between miR-18b expression and clinicopathological features of patients with gastric cancer.

miR-18b expression

Clinicopathological featuresNumber of casesHighLowP-value
Age (years) 0.282
  <6018117
  ≥60311417
Sex 0.322
  Male281612
  Female21912
Tumor size (cm) 0.458
  <421129
  ≥4281315
Differentiation 0.477
  Well and moderate241113
  Poor and signet251411
Lymph node metastasis 0.032a
  No23815
  Yes26179
Invasive depth 0.015b
  T1+T226917
  T3+T423167
TNM stage 0.015c
  I–II20614
  III–IV291910

{ label (or @symbol) needed for fn[@id='tfn1-mmr-19-03-1926'] } miR-18b, microRNA-18b; TNM, tumor-node-metastasis.

a P<0.05 vs. lymph node metastasis (No).

b P<0.05 vs. invasive depth (T1+T2).

c P<0.05 vs. TNM stage (I–II).

Downregulation of miR-18b inhibits proliferation and invasion of AGS and BGC-823 cells

To investigate the role of miR-18b in GC, AGS and BGC-823 cells were transfected with miR-18b inhibitor to decrease its expression. Following transfection, RT-qPCR revealed that miR-18b was significantly downregulated in cells transfected with miR-18b inhibitor compared with the cells transfected with NC inhibitor (Fig. 2A; P<0.05). A CCK-8 assay was subsequently conducted to examine the effect of miR-18b knockdown on GC cell proliferation. The downregulation of miR-18b significantly suppressed the proliferation of AGS and BGC-823 cells (Fig. 2B; P<0.05). Additionally, a cell invasion assay was performed to evaluate cell invasion ability in cells transfected with miR-18b inhibitor or NC inhibitor. Transfection with miR-18b inhibitor markedly attenuated the invasion capacities of AGS and BGC-823 cells compared with the NC inhibitor group (Fig. 2C; P<0.05). Taken together, this indicated that miR-18b may have an oncogenic role in GC progression.

KLF6 is a direct target of miR-18b in GC

To elucidate the mechanism underlying the oncogenic role of miR-18b in GC, bioinformatics analysis was used to predict the potential targets of miR-18b. KLF6, which has previously been reported to affect GC initiation and progression (1921), was predicted as a putative target of miR-18b. As presented in Fig. 3A, the 3′-UTR of KLF6 contains two miR-18b binding sites (1 and 2). A luciferase reporter assay was performed to investigate if miR-18b interacted with the 3′-UTR of KLF6. miR-18b downregulation increased the luciferase activity of pmirGLO-KLF6-3′-UTR Wt (1 and 2) in AGS and BGC-823 cells (Fig. 3B; P<0.05). However, no significant changes in luciferase activity were detected in cells transfected with pmirGLO-KLF6-3′-UTR Mut (1 and 2) in the presence or absence of miR-18b inhibitor. Furthermore, RT-qPCR and western blot analysis were performed to investigate if miR-18b regulated KLF6 expression in GC cell lines. Transfection of miR-18b inhibitor increased the expression of KLF6 expression at both mRNA (P<0.05; Fig. 3C) and protein (P<0.05; Fig. 3D) levels in AGS and BGC-823 cells. Collectively, this data indicated that KLF6 may be a direct target of miR-18b in GC.

KLF6 is downregulated in GC tissues and inversely correlates with miR-18b expression

To further explore the association between miR-18b and KLF6 in GC, the expression of KLF6 in 49 pairs of GC and matched adjacent normal gastric tissues was detected. RT-qPCR and western blot analysis revealed that KLF6 expression was downregulated in GC tissues compared with adjacent normal gastric tissues at the mRNA (Fig. 4A; P<0.05) and protein (Fig. 4B) level. Additionally, a negative association between miR-18b and KLF6 mRNA expression in GC tissues was confirmed by Spearman's correlation analysis (Fig. 4C; r=−0.6030; P<0.0001). These results suggested that the downregulation of KLF6 in GC may be attributed at least in part to the upregulation of miR-18b.

KLF6 knockdown partially rescues the effects of miR-18b inhibitor on proliferation and invasion of AGS and BGC-823 cells

Rescue experiments were performed to further confirm that the effect of miR-18b knockdown on GC cell proliferation and invasion is mediated by the regulation of KLF6 expression. AGS and BGC-823 cells were transfected with miR-18b inhibitor in combination with KLF6 siRNA or NC siRNA. Western blot analysis indicated that co-transfection of KLF6 siRNA partially abrogated the miR-18b inhibitor-mediated upregulation of KLF6 (Fig. 5A; P<0.05). Additionally, functional experiments demonstrated that KLF6 restoration rescued the inhibition of cell proliferation (Fig. 5B; P<0.05) and invasion (Fig. 5C; P<0.05) caused by miR-18b knockdown in AGS and BGC-823 cells. Collectively, these results suggested that the oncogenic roles of miR-18b in GC are achieved, at least in part, by regulation of KLF6 expression.

Discussion

An increasing number of studies have demonstrated that miRNAs contribute to gastric cancer development and progression (2224). Therefore, investigation of the miRNAs involved in the development of GC may provide an effective therapeutic target for patients with this malignancy. In the present study, miR-18b was significantly upregulated in GC tissues and cell lines. High miR-18b expression was associated with lymph node metastasis, invasive depth and TNM stage in patients with GC. Inhibition of miR-18b prohibited the proliferation and invasion of GC cells. Additionally, KLF6 was identified as a direct target of miR-18b in GC. KLF6 was downregulated in GC and negatively correlated with miR-18b expression level. Furthermore, KLF6 restoration partially rescued the effects of miR-18b inhibition on the proliferation and invasion of GC cells. Therefore, targeting of miR-18b may be developed as a potential therapeutic strategy in the treatment of patients with GC.

miR-18b expression is dysregulated in several types of human cancer. miR-18b is downregulated in melanoma tissues and cell lines, and decreased levels are also significantly associated with tumor thickness and stage (15). Additionally, miR-18b has been reported to be upregulated in colorectal cancer. High expression of miR-18b is significantly associated with lymph node and distant metastasis of patients with colorectal cancer (16). Expression of miR-18b is also higher in poorly differentiated hepatocellular carcinoma (HCC), compared with that in well-differentiated HCC. Following surgery resection, patients with HCC and high miR-18b expression have a shorter relapse-free period than those patients with low expression (17). In mantle cell lymphoma, miR-18b is overexpressed and associated with a poor survival rate (25). Elevated miR-18b expression is also reported in nasopharyngeal carcinoma (26) and breast cancer (27). These findings indicate that the expression pattern of miR-18b possesses tissue specificity and may serve as a useful prognostic marker in human cancers.

Numerous studies have reported that miR-18b contributes to the malignant phenotype of cancers. Upregulation of miR-18b has been demonstrated to inhibit melanoma cell proliferation, migration, invasion, glycolysis and epithelial-to-mesenchymal transition in vitro, increase apoptosis in vitro and reduce tumor growth in vivo (15,28). miR-18b has been validated as an oncogene in colorectal cancer via participation in the regulation of cell cycle, proliferation and migration (16). Murakami et al (17) reported that the upregulation of miR-18b promotes cell proliferation and inhibits cell adhesion capacity in HCC. Fonseca-Sanchéz et al (27) demonstrated that the downregulation of miR-18b attenuates breast cancer cell migration in vitro. These results suggest that biological roles of miR-18b also have tissue specificity and further indicate the potential of miR-18b as a therapeutic target for the treatment of certain cancer types.

Several targets of miR-18b have been identified, including hypoxia inducible factor 1 a (15) and MDM2 (28) in melanoma, cyclin dependent kinase inhibitor 2B (16) in colorectal cancer and trinucleotide repeat containing 6B (17) in HCC. In the present study, KLF6 was validated as a novel target of miR-18b in GC. KLF6, a zinc finger transcription factor, is frequently downregulated in several types of human cancer, including lung (29), ovarian (30), prostate (31), glioma (32) and colorectal cancer (33). Previous studies have demonstrated that KLF6 contributes to the regulation of various biological processes, including cell proliferation, apoptosis, differentiation, invasion and metastasis (34,35). In GC, the expression level of KLF6 is decreased in tumor tissues compared with normal gastric mucosa (18). Low KLF6 expression is strongly associated with histological differentiation, TNM stage, lymph node metastasis and distant metastasis (19). KLF6 overexpression reduces cell proliferation, colony formation, metastasis and increases apoptosis in GC (20,21). Considering the important roles of KLF6 in GC, targeting KLF6 may provide an opportunity to inhibit tumor formation and development in GC.

In conclusion, miR-18b was upregulated in GC tissues and cell lines. Its high expression was significantly associated with lymph node metastasis, invasive depth and TNM stage in patients with GC. miR-18b may have an oncogenic role in GC partly through direct targeting of KLF6, suggesting that the miR-18b/KLF6 axis may be a potential therapeutic target for treating patients with GC.

Acknowledgements

Not applicable.

Funding

This study was supported by the National Natural Science Foundation of China (grant no. 81360370), the Natural Science Foundation of Guangxi Zhuang Autonomous Region (grant no. 2017AB45153), the Education Department Foundation for Innovation Team of Guangxi Zhuang Autonomous Region and the self-financing project of the Guangxi Zhuang Autonomous Region Health and Family Planning Commission (grant no. Z 20170908).

Availability of data and materials

The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.

Authors' contributions

DL and JC designed the present study. DL, SH, JX, XS and PY performed functional experiments. All authors have read and approved the final draft.

Ethics approval and consent to participate

The present study was approved by the Research Ethics Committee of The First Affiliated Hospital of Guangxi Medical University (Nanning, China), and was performed in accordance with the Declaration of Helsinki and the guidelines of the Ethics Committee of The First Affiliated Hospital of Guangxi Medical University.

Consent for publication

Written informed consent was obtained from all patients for the use of their clinical tissues.

Competing interests

The authors declare that they have no competing interests.

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March-2019
Volume 19 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Luo D, Chen J, Huang S, Xu J, Song X and Yu P: MicroRNA‑18b acts as an oncogene in gastric cancer by directly targeting Kruppel‑like factor 6 Retraction in /10.3892/mmr.2022.12916. Mol Med Rep 19: 1926-1934, 2019.
APA
Luo, D., Chen, J., Huang, S., Xu, J., Song, X., & Yu, P. (2019). MicroRNA‑18b acts as an oncogene in gastric cancer by directly targeting Kruppel‑like factor 6 Retraction in /10.3892/mmr.2022.12916. Molecular Medicine Reports, 19, 1926-1934. https://doi.org/10.3892/mmr.2019.9830
MLA
Luo, D., Chen, J., Huang, S., Xu, J., Song, X., Yu, P."MicroRNA‑18b acts as an oncogene in gastric cancer by directly targeting Kruppel‑like factor 6 Retraction in /10.3892/mmr.2022.12916". Molecular Medicine Reports 19.3 (2019): 1926-1934.
Chicago
Luo, D., Chen, J., Huang, S., Xu, J., Song, X., Yu, P."MicroRNA‑18b acts as an oncogene in gastric cancer by directly targeting Kruppel‑like factor 6 Retraction in /10.3892/mmr.2022.12916". Molecular Medicine Reports 19, no. 3 (2019): 1926-1934. https://doi.org/10.3892/mmr.2019.9830