Emodin inhibits the progression of acute pancreatitis via regulation of lncRNA TUG1 and exosomal lncRNA TUG1
- Authors:
- Published online on: September 8, 2021 https://doi.org/10.3892/mmr.2021.12425
- Article Number: 785
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Copyright: © Wen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Acute pancreatitis (AP) is a type of inflammatory disease, which results from the dysregulation of pancreatic enzymes in the pancreas (1,2). AP is very common, has a considerably high mortality rate and can cause systemic complications (3). The mortality rate of AP has increased from 15 to 30% (4), while the combination of AP with sepsis increases the mortality rate to >50% (5). Although significant efforts have been made to prevent and treat AP (6), no specific treatment methods are currently available. The development of the inflammatory response in pancreatic acinar cells correlates with the progression of AP (7). Therefore, inhibition of the inflammation in pancreatic acinar cells is crucial for the treatment of AP.
Emodin (1,3,8-Trihydroxy-6-methylanthra-quinone) is a natural product, which originates from Rheum palmatum. Previous studies have reported that emodin exhibits anti-inflammatory effects (8,9). In addition, it has been revealed that emodin can alleviate the symptoms of AP (10). However, the underlying mechanism by which emodin regulates the progression of AP remains unclear.
Long non-coding RNAs (lncRNAs) can play important roles in the development of multiple diseases (11,12). Moreover, lncRNAs participate in the prevention of AP. For example, Zhu et al (13) demonstrated that lncRNA maternally expressed 3 alleviated caerulein-induced inflammatory injury in human pancreatic cells. In addition, lncRNA cancer susceptibility 2 has been reported to be involved in the progression of AP (14). Furthermore, the expression levels of lncRNA taurine upregulated 1 (TUG1) are upregulated in pancreatic tissues (15). However, the role of TUG1 in the development of AP is yet to be fully elucidated.
Exosomes are a type of extracellular vesicles, which can be secreted by cells and promote the transfer of molecules to cells (16,17). In addition, exosomes play key roles in the progression of multiple diseases (18,19). A previous report indicated that exosomes can regulate the progression of AP (20). However, the underlying mechanism requires further investigation.
It has been reported that dysregulation of immune responses may lead to the progression of AP (21,22). Guo et al (23) highlighted that the T helper 17 (Th17) cell/regulatory T cell (Treg) imbalance was involved in the development of AP and that it may be correlated with its severity and prognosis (24). Therefore, Treg cells play a key role in mediating the progression of AP. In addition, it has been shown that TUG1 regulates Treg cell differentiation (25). Based on this evidence, the present study aimed to explore the association between emodin and Treg cells in AP.
In the current study, the effects of emodin on AR42J cells cotreated with caerulein and lipopolysaccharide (LPS) were investigated. The present study aimed to provide a novel treatment strategy for AP.
Materials and methods
Cell culture
Rat pancreatic acinar cell lines (AR42J) were purchased from Tongpai (Shanghai) Biotechnology Co., Ltd. and maintained in DMEM (Thermo Fisher Scientific, Inc.) containing 10% FBS (26), 1% penicillin and 1% streptomycin (Thermo Fisher Scientific, Inc.) at 37°C with 5% CO2. To mimic AP in vitro, AR42J cells were treated with LPS (10 µg/ml; Beijing Solarbio Science & Technology Co., Ltd.) and caerulein (100 nM; Beijing Solarbio Science & Technology Co., Ltd.) for 3 h, as previously described (27).
Cell transfection
AR42J cells (3×105 cells/well) were transfected with pcDNA3.1 (negative control; NC; 1 µg/µl) or pcDNA3.1-TUG1 [1 µg/µl, TUG1 overexpression (OE)] using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 48 h. pcDNA3.1 and pcDNA3.1-TUG1 were purchased from Shanghai GenePharma Co., Ltd. After 48 h of transfection, the transfected cells were used for subsequent analysis.
Exosome isolation
Briefly, AR42J cells were cotreated with LPS (10 µg/ml) and caerulein (100 nM) at 37°C for 3 h. Then, AR42J cells (3×105 cells/well) or AR42J cells cotreated with LPS and caerulein were centrifuged at 300 × g for 15 min, 2,000 × g for 15 min, and 10,000 × g for 30 min at room temperature to collect the supernatant. The samples were filtered with a 0.22-µm filter and collected to isolate exosomes via ultracentrifugation at 4°C (120,000 × g for 70 min). For collection of the pellet, the samples were centrifuged at 10,000 × g for 1 h at 4°C. Then, the pellet was resuspended in PBS for further analysis. Meanwhile, the particle sizes of exosomes were investigated by Nanoparticle Tracking Analysis (NTA), the structure of exosomes was observed by transmission electron microscopy (TEM) and the exosome markers were detected by western blotting. Exosomes isolated from AR42J cells were the control-exo group; exosomes isolated from AR42J cells cotreated with LPS and caerulein were the AP-exo group.
Reagents
Emodin was purchased from Beijing Solarbio Science & Technology Co., Ltd. IL-2 (cat. no. SRP3242) and anti-CD3 (cat. no. SAB4700040)/CD28 (cat. no. SAB4700739) were purchased from Sigma-Aldrich; Merck KGaA. Caerulein was obtained from MedChemExpress. Meanwhile, ARJ21 cells were treated with 20 and 40 µM emodin, according to previous references (28,29).
TEM
The exosome pellet was incubated for 5 min and subsequently immersed in 2% phosphotungstic acid solution for 1 min. The pellet was fixed using 2.5% glutaraldehyde (pH 7.2) at 4°C overnight. Then, 100 µl suspension was placed on a parafilm sheet and a copper grid coated with carbon was placed onto the drop for 10 sec and then removed. The grid was then rinsed 10 times with MiliQ H2O (1 min each) at room temperature. Subsequently, the grid was laid on a drop of uranyl acetate (pH 7.0; cat. no. 2624; SPI-CHEM) at room temperature for 10 min. After rinsing with Milli-Q H2O and methylcellulose uranyl (pH 4.0), the grid was incubated at room temperature for 10 min on a drop of methylcellulose uranyl (pH 4.0, cat. no. M-6385; Sigma-Aldrich; Merck KGaA). Finally, the grid was dried for 10 min at room temperature and observed using a transmission electron microscope (JEOL, Ltd.).
Cell Counting Kit-8 (CCK-8) assay
The viability of AR42J cells (5×103/well) was determined in each group using the CCK-8 assay (Beyotime Institute of Biotechnology). In brief, ARJ21 cells were plated (5×103 cells/well) into 96-well plates and treated for 0, 24, 48 or 72 h at 37°C. Subsequently, cells were incubated with 10 µl CCK-8 reagents for 2 h at 37°C. The optical density values were measured at 450 nm using a microplate reader to assess cell viability.
Cell apoptosis analysis
The early + late apoptosis of AR42J cells was detected by flow cytometry. In brief, AR42J cells (1×104 per well) were trypsinized, washed with PBS and resuspended in binding buffer. Subsequently, the cells were stained with 5 µl Annexin V-FITC and PI (BD Biosciences) in the dark at 37°C for 30 min. Flow cytometry (FACScan™; BD Biosciences) was applied to investigate the apoptotic rate using FlowJo (version 10.6.2; BD Biosciences).
Western blot analysis
Total protein was isolated from cell lysates using RIPA buffer (Beyotime Institute of Biotechnology). The protein was quantified by the BCA protein kit (Thermo Fisher Scientific, Inc.). Subsequently, the proteins (30 µg/lane) were separated with SDS-PAGE gel (10%) and separated proteins were subsequently transferred to PVDF membranes, which were incubated with primary antibodies overnight at 4°C following blocking with 3% non-fat milk at room temperature for 1 h. The membranes were incubated with Goat Anti-Rabbit antibody (HRP-conjugated, 1:5,000; cat. no. ab7090; Abcam) at room temperature for 1 h, scanned by an Odyssey Imaging System (Thermo Fisher Scientific, Inc.) and analyzed with ImageJ software (version 1.8.0; National Institutes of Health). The primary antibodies used in the present study were as follows: Anti-CD63 (1:1,000; cat. no. ab134045; Abcam), anti-Calnexin (1:1,000; cat. no. ab133615; Abcam), anti-IL-10 (1:1,000; cat. no. ab133575; Abcam), anti-TGF-β (1:1,000; cat. no. ab215715; Abcam) and anti-β-actin (1:1,000; cat. no. ab8226; Abcam). All the antibodies used were purchased from Abcam.
Reverse transcription-quantitative PCR (RT-qPCR)
Total RNA from cells or exosomes was isolated using the TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and the exoRNeasy Midi kit (Qiagen, Inc.). The All-in-One™ First-Strand cDNA Synthesis kit (GeneCopoeia, Inc.) was used to transcribe total RNA into cDNA according to the manufacturer's protocol. RT-qPCR was performed using the SYBR™ Green Master Mix (Qiagen, Inc.). qPCR was performed in triplicate under the following protocol: 2 min at 94°C, followed by 35 cycles for 30 sec at 94°C and 45 sec at 55°C. The 2−ΔΔCq method (30) was used to quantify the data and GAPDH was used for normalization. The primer sequences used were as follows: TUG1 forward (F), 5′-ATCTAATCAGTAAGCGGA-3′ and reverse (R), 5′-AAAGCAAGTCAAGACCTC-3′; IL-10 F, 5′-GAAAAATTGAACCACCCGGCA-3′ and R, 5′-TTCCAAGGAGTTGCTCCCGT-3′; TGF-β F, 5′-CTGCTGACCCCCACTGATAC-3′ and R, 5′-AGCCCTGTATTCCGTCTCCT-3′; and GADPH F, 5′-ACAGCAACAGGGTGGTGGAC-3′ and R, 5′-TTTGAGGGTGCAGCGAACTT-3′.
NTA
The hydrodynamic radius and concentration of exosomes were detected via NTA, as previously described (31). In brief, a total of ~0.3 ml supernatant was loaded into the sample chamber of an LM10 Nanosight unit (Nanosight, Ltd.) and three videos of either 30 or 60 sec were recorded of each sample. Data analysis was performed using NTA 2.1 software (Nanosight, Ltd.). The paths of unlabeled particles acting as point scatterers undergoing Brownian motion in a 0.25 ml chamber through which a 635-nm laser beam is passed were determined from a video recording, with the mean squared displacement determined for each possible particle. The diffusion coefficient and sphere-equivalent hydrodynamic radius were subsequently determined using the Stokes-Einstein equation.
ELISA
The levels of IL-6 (cat. no. SEA079Ra), IL-1β (cat. no. SEA563Ra) and TNF-α (cat. no. SEA133Ra) in the serum of mice or the cell supernatants were detected using ELISA kits (Wuhan USCN Business Co., Ltd.). The cell supernatants were harvested by centrifugation (500 × g, 4°C, 10 min). Subsequently, cells were incubated with a secondary antibody (cat. no. BA1054; Wuhan Boster Biological Technology, Ltd.) at room temperature for 1 h. Finally, following incubation with hydrochloric acid at room temperature for 5 min, the absorbance was measured using a microplate reader.
Animal experiments
A male Wistar rat (12-weeks-old, 200 g) was obtained from The Chinese Academy of Sciences. The rat was housed within a dedicated specific pathogen-free (SPF) facility (it was raised in standard cages with 12-h light/dark cycle, a constant temperature of 23±1°C and a humidity of 50–60%) and had free access to food and water. The protocols for care and use of laboratory animals were approved by the Zhejiang Chinese Medical University (Hangzhou, China). The rat was euthanized using CO2 at a displacement rate of 30% of the chamber volume/min (CO2 flow rate, 2.5 l/min). Then, peripheral blood mononuclear cells (PBMCs, 5 ml) were collected. PBMCs were collected as described in a previous study (32). In brief, CD4+T cells were isolated from PBMCs, and then CD4+T cells were treated with 10 ng/ml IL-2 and 2 µg/ml anti-CD3/CD28 for 72 h in order to induce the activation of Treg cells (33).
BALB/c mice (n=18, male, 28–35 g; age, 6–8 weeks) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. All mice were maintained under SPF conditions. All procedures performed in this study involving animals were in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (34). The animal experiments were approved by the Animal Care and Use Committee of The First Affiliated Hospital of Zhejiang Chinese Medical University (approval no. 20210201003B). To mimic AP in vivo, mice were fasted for 12 h, and a mouse model of AP was constructed according to a previous method (35). Briefly, mice in the AP group were injected intraperitoneally with 50 µg/kg caerulein 12 times (0.2 ml/mice, each time interval was 1 h). In addition, mice in the control group were injected with saline with the same procedure. Meanwhile, before the second injection and after the eighth injection of caerulein, emodin (40 mg/kg) was injected intraperitoneally into mice (AP + emodin group). The mice were sacrificed 48 h after the last injection of caerulein, and the tissues and serum were then collected. All mice were euthanized using CO2 at a displacement rate of 30% of the chamber volume/min (CO2 flow rate, 2.5 l/min).
To evaluate the effects of emodin in AP mice, inflammatory factor (IL-1β, IL-6 and TNF-α) contents in serum of mice were detected with the ELISA kits, as mentioned above.
Hematoxylin and eosin (H&E) staining
H&E staining was used to observe pancreatic injury and inflammation as previously described (36). Paraffin-embedded tissues were cut to 4 µm thickness, deparaffinized, rehydrated and stained with H&E using standard procedures. The slides were then mounted with BioMount mounting media and observed under a light microscope (Olympus Corporation).
Isolation of CD4+ T cells
A total of 2×106 PBMCs/ml were seeded in 6-well cell culture plates. The cells were treated with 50 ng/ml Phorbol-12-myristate-13-acetate (Beijing Solarbio Science & Technology Co., Ltd.), Ionomycin (2 µg/ml; Beijing Solarbio Science & Technology Co., Ltd.) and Brefelin (2 µg/ml; Beijing Solarbio Science & Technology Co., Ltd.). These reagents were incubated with the cells at 37°C for 4 h. The CD4+T cell population was isolated from the cell cultures by immunomagnetic selection (MidiMACS™ Separator; Thermo Fisher Scientific, Inc.).
Flow cytometry
To further induce the differentiation of Treg cells, CD4+T cells (5×103/well) were treated with anti-CD3 (cat. no. SAB4700040)/anti-CD28 (cat. no. SAB4700739; 2 µg/ml; Sigma-Aldrich; Merck KGaA) and IL-2 (10 ng/ml; Sigma-Aldrich; Merck KGaA) at 37°C for 72 h. The cell suspension was collected and the cells were incubated with anti-CD4 (labeled with FITC; cat. no. 11-0040-82; Thermo Fisher Scientific, Inc.) and anti-CD25 (labeled with PE; cat. no. MA1-90766; Thermo Fisher Scientific, Inc.) for 30 min. Subsequently, the cells were centrifuged at 3,000 × g at 4°C for 10 min, washed and fixed with 4% paraformaldehyde for 20 min at 4°C. The samples were resuspended and incubated with anti-FOXP3 antibody (labeled with APC; cat. no. 17-5773-82; Thermo Fisher Scientific, Inc.) for 30 min at 4°C. Finally, the cells were centrifuged at 3,000 × g at 4°C for 10 min, washed, fixed with 4% paraformaldehyde at 4°C for 10 min and the ratio of CD4+/CD25+/FOXP3+ T cells was measured by FACS (FACSLyric™; BD Biosciences). FlowJo (version 10.6.2; BD Biosciences) was used to analyze the data.
Statistical analysis
All data are expressed as the mean ± SEM. The CCK-8 assay was performed five times. Western blotting, ELISA, RT-qPCR and flow cytometry assays were performed in triplicate. One-way ANOVA followed by the Tukey's post hoc test were used for comparisons between ≥3 groups. P<0.05 was considered to indicate a statistically significant difference.
Results
AP in vitro model
To mimic AP in vitro, AR42J cells were cotreated with caerulein and LPS. The levels of IL-1β and TNF-α in the supernatants of AR42J cells were significantly increased following treatment of the cells with caerulein and LPS (Fig. 1A and B). The data suggested that the in vitro model of AP was successfully established.
lncRNA TUG1 expression is upregulated in AR42J cells cotreated with caerulein and LPS and in exosomes derived from caerulein and LPS pretreated AR42J cells
The separation efficiency of exosomes was examined by TEM. Exosomes demonstrated disc-shaped crescent-shaped and double-layered membrane structure (Fig. 2A). In addition, the expression levels of CD63 were notably higher in the AP-exo group than those noted in the control-exo, while western blotting demonstrated the absence of calnexin expression in the control-exo and AP-exo groups (Fig. 2B). In addition, rounded particles of ~80–100 nm in diameter were noted following treatment of the cells with LPS and caerulein (Fig. 2C). The data suggested that exosomes were successfully separated from AR42J cells. Furthermore, the expression levels of TUG1 in AR42J cells and exosomes derived from AR42J cells were significantly increased following treatment of the cells with caerulein and LPS (Fig. 2D and E). In summary, the data indicated that lncRNA TUG1 expression was upregulated in caerulein and LPS pre-treated AR42J cells and exosomes derived from AR42J cells cotreated with caerulein and LPS.
Emodin inhibits the expression of lncRNA TUG1 in cells cotreated with LPS and caerulein and exosomes derived from the aforementioned cell samples
The effects of emodin on AR42J cell viability were assessed using the CCK-8 assay. The viability of AR42J cells was significantly inhibited by caerulein and LPS, while the effects of caerulein and LPS were reversed following treatment of the cells with 20 or 40 µM emodin (Fig. 3A). In addition, the apoptotic rate of AR42J cells cotreated with caerulein and LPS was significantly inhibited following treatment of the cells with 20 or 40 µM emodin (Fig. 3B). Moreover, ELISA demonstrated that the levels of IL-1β and TNF-α in supernatants derived from AR42J cells were significantly increased following treatment of the cells with caerulein and LPS, while application of 20 or 40 µM emodin significantly reversed this effect (Fig. 3C and D). Furthermore, caerulein and LPS significantly increased the levels of TUG1 in AR42J cells or exosomes derived from AR42J cells, while their effects were significantly reversed by emodin treatment of the cells (20 or 40 µM; Fig. 3E and F). Since AR42J cells were more sensitive to 40 µM emodin, this concentration was selected for subsequent analysis. Taken together, the data indicated that emodin inhibited the expression levels of cellular and exosomal TUG1 in AR42J cells cotreated with caerulein and LPS.
TUG1 overexpression induces apoptosis and significantly reverses the effects of emodin on the viability of AR42J cells, which were pretreated with caerulein and LPS
To investigate the effects of TUG1 on emodin-treated AR42J cells, cells were transfected with a TUG1 OE plasmid. The expression levels of TUG1 were upregulated following transfection of AR42J cells with TUG1 OE (Fig. 4A). Moreover, overexpression of TUG1 caused a significant reduction in the emodin-induced increase of AR42J cell viability following treatment with caerulein and LPS (Fig. 4B). The inhibitory effect of emodin on the induction of apoptosis in AR42J cells cotreated with caerulein and LPS was significantly reduced following overexpression of TUG1 (Fig. 4C). In conclusion, TUG1 overexpression reversed the effects of emodin on the proliferation of AR42J cells cotreated with caerulein and LPS by inducing cell apoptosis.
Emodin promotes the differentiation of Treg cells by inhibition of TUG1 in exosomes derived from AR42J cells cotreated with caerulein and LPS
In order to confirm whether emodin mediates Treg cell differentiation via regulation of exosomal TUG1, RT-qPCR was performed. The expression levels of TUG1 in CD4+T cells cotreated with anti-CD3/anti-CD28 and IL-2 were significantly increased by AP-exo (exosomes derived from AR42J cells cotreated with LPS and caerulein), while the effects of AP-exo were reversed by emodin treatment (Fig. 5A). In addition, the ratio of CD4+/CD25+/FOXP3+ T cells in CD4+ T cells was significantly increased following their combined treatment with anti-CD3/anti-CD28 and IL-2, while this effect was reversed by AP-exo treatment (Fig. 5B and C). Moreover, emodin significantly inhibited the effects of AP-exo on Treg cell differentiation (Fig. 5B and C). The expression levels of IL-10 and TGF-β in CD4+T cells were notably increased by combined treatment of anti-CD3/anti-CD28 and IL-2, while these effects were partially reversed by AP-exo treatment (Fig. 5D-F). However, emodin restored the effects of anti-CD3/anti-CD28 and IL-2 (Fig. 5D-F). In summary, emodin may promote the differentiation of Treg cells by inhibition of exosomal lncRNA TUG1 expression.
Emodin significantly inhibits the progression of AP in vivo
To further confirm the function of emodin in AP, an in vivo model of AP was established. As indicated in Fig. 6A, notable injury of the pancreas tissue and inflammatory infiltration were observed in AP mice, while emodin markedly reversed this phenomenon. In addition, AP-induced upregulation of IL-6, IL-1β and TNF-α was significantly inhibited in the presence of emodin (Fig. 6B). Overall, emodin significantly inhibited the progression of AP in vivo.
Discussion
A number of agents have been reported to have anti-inflammatory effects on AP. For instance, Lin et al (37) found Flos Lonicerae Japonicae water extract (FLJWE) could inhibit pseudorabies virus-induced inflammation in RAW264.7 cells. Furthermore, Esmail et al (38) indicated that niclosamide could inhibit inflammation in liver fibrosis. Meanwhile, it has been reported that emodin exhibits anti-inflammatory effects on AP (10,39). In the present study, the results indicated that this compound could inhibit the inflammatory response in AR42J cells cotreated with LPS and caerulein. Moreover, the present study demonstrated that emodin promoted Treg cell differentiation in AP by regulation of TUG1 in exosomes derived from AR42J cells cotreated with caerulein and LPS. Meanwhile, compared with other anti-inflammatory agents (for example, FLJWE and niclosamide), emodin has been confirmed to significantly relieve inflammation of the gastrointestinal tract (40,41). Based on the present data and previous reports, the findings demonstrated the underlying mechanism by which emodin mediated the progression of AP, suggesting that it could act as an inhibitor of AP.
lncRNAs are involved in the progression of AP (1,42). The current study demonstrated that TUG1 expression was upregulated in AR42J cells cotreated with caerulein and LPS, suggesting that TUG1 may act as a promoter of AP. Moreover, TUG1 has been shown to regulate disease progression by sponging microRNAs (miRNAs/miRs). For example, Tang et al (43) demonstrated that TUG1 promoted the function of oxidized low-density lipoprotein-treated human aortic vascular smooth muscle cells by activation of the miR-141-3p/receptor tyrosine kinase like orphan receptor 2 axis. Moreover, Pei et al (44) indicated that TUG1 mediated the proliferation and migration of ovarian cancer cells by sponging miR-1299. Therefore, the potential target miRNAs of TUG1 should be investigated in future studies.
Exosomes are known to be secreted by multiple types of cells and may be associated with the progression of multiple diseases (45,46). Accumulating evidence has indicated that exosomes can create an immune suppressive environment by inducing inflammation (47,48). Wang et al (49) suggested that exosomes from mesenchymal stem cells that overexpressed Klotho could reverse apoptosis and NF-kB activation in caerulein-stimulated AR42J cells. Similarly, the findings suggested that exosomes derived from AR42J cells caused upregulation of the expression levels of TUG1 in order to inhibit the immune response during the progression of AP. Furthermore, Salminen et al (50) highlighted that exosomal vesicles enhanced immunosuppression during chronic inflammation by regulation of Treg cell differentiation. The present findings demonstrated that exosomes derived from AR42J cells cotreated with LPS and caerulein reduced the number of CD4+CD25+FOXP3+ T cells, which may lead to the induction of inflammation. Taken together, the data indicated that exosomes were important components of the inflammatory response and acted as important messengers, which mediated the cross-talk between cells. Therefore, emodin promoted Treg cell differentiation by regulating the function of exosomes derived from AR42J cells cotreated with caerulein and LPS.
The current study has the following limitations: i) The underlying mechanism by which exosomes mediated Treg cell differentiation was not fully investigated; ii) the ratio of Th17/Treg cells was not assessed; iii) the mechanism by which emodin regulated TUG1 expression requires further investigation; and iv) the expression of FOXP3 in CD4+CD25+ cells and the effect of emodin on FOXP3 expression in terms of differentiation require further confirmation. Thus, additional investigations are required in future studies.
In summary, the data of the present study indicated that emodin inhibited the progression of AP in vitro by regulation of the expression levels of cellular and exosomal lncRNA TUG1. Therefore, emodin may be useful as a novel agent for the treatment of AP.
Acknowledgements
Not applicable.
Funding
This research was supported by the foundation of State Administration of Traditional Chinese Medicine of Zhejiang Province (grant no. 2016ZB064) and Medical Health Science and Technology Project of Zhejiang Provincial Health Commission (grant no. 2019KY478).
Availability of data and materials
All data generated or analyzed during this study are included in this published article.
Authors' contributions
ZC conceived and supervised the present study. XW designed the experiments. XW, BH, XT and BW performed the experiments. ZC and XW confirm the authenticity of all the raw data. All authors reviewed the results. All authors have read and approved the final version of the manuscript.
Ethics approval and consent to participate
All procedures performed in this study involving animals were in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. The animal experiments were approved by the Animal Care and Use Committee of The First Affiliated Hospital of Zhejiang Chinese Medical University (approval no. 20210201003B; Hangzhou, China).
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
References
|
Song G, Zhou J, Song R, Liu D, Yu W, Xie W, Ma Z, Gong J, Meng H, Yang T and Song Z: Long noncoding RNA H19 regulates the therapeutic efficacy of mesenchymal stem cells in rats with severe acute pancreatitis by sponging miR-138-5p and miR-141-3p. Stem Cell Res Ther. 11:4202020. View Article : Google Scholar : PubMed/NCBI | |
|
Numoto I, Tsurusaki M, Oda T, Yagyu Y, Ishii K and Murakami T: Transcatheter arterial embolization treatment for bleeding visceral artery pseudoaneurysms in patients with pancreatitis or following pancreatic surgery. Cancers (Basel). 12:27332020. View Article : Google Scholar : PubMed/NCBI | |
|
Barreto SG, Habtezion A, Gukovskaya A, Lugea A, Jeon C, Yadav D, Hegyi P, Venglovecz V, Sutton R and Pandol SJ: Critical thresholds: Key to unlocking the door to the prevention and specific treatments for acute pancreatitis. Gut. 70:194–203. 2021. View Article : Google Scholar : PubMed/NCBI | |
|
Yu B, Li J, Li N, Zhu Y, Chen Y, He W and Lu N: Progression to recurrent acute pancreatitis after a first attack of acute pancreatitis in adults. Pancreatology. 20:1340–1346. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Ko J, Skudder-Hill L, Cho J, Bharmal SH and Petrov MS: The relationship between abdominal fat phenotypes and insulin resistance in non-obese individuals after acute pancreatitis. Nutrients. 12:e17912021. | |
|
Kurihara Y, Maruhashi T, Wada T, Osada M, Oi M, Yamaoka K and Asari Y: Pancreatitis in a patient with severe coronavirus disease pneumonia treated with Veno-venous extracorporeal membrane oxygenation. Intern Med. 59:2903–2906. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Yang SR, Hsu WH, Wu CY, Shang HS, Liu FC, Chen A, Hua KF and Ka SM: Accelerated, severe lupus nephritis benefits from treatment with honokiol by immunoregulation and differentially regulating NF-κB/NLRP3 inflammasome and sirtuin 1/autophagy axis. FASEB J. 34:13284–13299. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Liu L, Lin Z, Zheng B, Wang L, Zou J, Wu S, Jiang Z, Jin Q, Lai X and Lin P: Reduced intellectual ability in offspring born from preeclamptic mothers: A Prospective cohort study. Risk Manag Healthc Policy. 13:2037–2046. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Guo R, Li Y, Han M, Liu J and Sun Y: Emodin attenuates acute lung injury in Cecal-ligation and puncture rats. Int Immunopharmacol. 85:1066262020. View Article : Google Scholar : PubMed/NCBI | |
|
Gao Z, Sui J, Fan R, Qu W, Dong X and Sun D: Emodin protects against acute Pancreatitis-Associated lung injury by inhibiting NLPR3 inflammasome activation via Nrf2/HO-1 signaling. Drug Des Devel Ther. 14:1971–1982. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Sun Z, Xue S, Zhang M, Xu H, Hu X, Chen S, Liu Y, Guo M and Cui H: Aberrant NSUN2-mediated m5 C modification of H19 lncRNA is associated with poor differentiation of hepatocellular carcinoma. Oncogene. 39:6906–6919. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Szikszai K, Krejcik Z, Klema J, Loudova N, Hrustincova A, Belickova M, Hruba M, Vesela J, Stranecky V, Kundrat D, et al: LncRNA profiling reveals that the deregulation of H19, WT1-AS, TCL6, and LEF1-AS1 Is associated with higher-risk myelodysplastic syndrome. Cancers (Basel). 12:27262020. View Article : Google Scholar : PubMed/NCBI | |
|
Zhu X, Qin X, Wang X, Wang Y, Cao W, Zhang J and Chen W: Oral cancer cellderived exosomes modulate natural killer cell activity by regulating the receptors on these cells. Int J Mol Med. 46:2115–2125. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Zeng J, Chen JY, Meng J and Chen Z: Inflammation and DNA methylation coregulate the CtBP-PCAF-c-MYC transcriptional complex to activate the expression of a long non-coding RNA CASC2 in acute pancreatitis. Int J Biol Sci. 16:2116–2130. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Xu K and Zhang L: Inhibition of TUG1/miRNA-299-3p axis represses pancreatic cancer malignant progression via suppression of the notch1 pathway. Dig Dis Sci. 65:1748–1760. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Gao Q, Fang X, Chen Y, Li Z and Wang M: Exosomal lncRNA UCA1 from cancer-associated fibroblasts enhances chemoresistance in vulvar squamous cell carcinoma cells. J Obstet Gynaecol Res. 47:73–87. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Zhang X, Gong W, Cao S, Yin J, Zhang J, Cao J and Shen Y: Comprehensive Analysis of Non-coding RNA profiles of exosome-like vesicles from the protoscoleces and hydatid cyst fluid of echinococcus granulosus. Front Cell Infect Microbiol. 10:3162020. View Article : Google Scholar : PubMed/NCBI | |
|
Wang D, Xing N, Yang T, Liu J, Zhao H, He J, Ai Y and Yang J: Exosomal lncRNA H19 promotes the progression of hepatocellular carcinoma treated with Propofol via miR-520a-3p/LIMK1 axis. Cancer Med. 9:7218–7230. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Yuan Z, Yang Z, Li W, Wu A, Su Z and Jiang B: Exosome-mediated transfer of long noncoding RNA HOTAIR regulates temozolomide resistance by miR-519a-3p/RRM1 axis in glioblastoma. Cancer Biother Radiopharm. Jul 24–2020.(Epub ahead of print). View Article : Google Scholar | |
|
Wu XB, Sun HY, Luo ZL, Cheng L, Duan XM and Ren JD: Plasma-derived exosomes contribute to pancreatitis-associated lung injury by triggering NLRP3-dependent pyroptosis in alveolar macrophages. Biochim Biophys Acta Mol Basis Dis. 1866:1656852020. View Article : Google Scholar : PubMed/NCBI | |
|
Yang L, Chen S, Zhao Q, Sun Y and Nie H: The critical role of Bach2 in shaping the balance between CD4+ T cell subsets in immune-mediated diseases. Mediators Inflamm. 2019:26097372019. View Article : Google Scholar : PubMed/NCBI | |
|
Qiao Y, Chen J, Ma C, Liu Y, Li P, Wang Y, Hou L and Liu Z: Increased KIF15 expression predicts a poor prognosis in patients with lung adenocarcinoma. Cell Physiol Biochem. 51:1–10. 2018. View Article : Google Scholar : PubMed/NCBI | |
|
Guo J, Li Z, Tang D and Zhang J: Th17/Treg imbalance in patients with severe acute pancreatitis: Attenuated by high-volume hemofiltration treatment. Medicine (Baltimore). 99:e214912020. View Article : Google Scholar : PubMed/NCBI | |
|
Wang D, Tang M, Zong P, Liu H, Zhang T, Liu Y and Zhao Y: miRNA-155 regulates the Th17/Treg ratio by targeting SOCS1 in severe acute pancreatitis. Front Physiol. 9:6862018. View Article : Google Scholar : PubMed/NCBI | |
|
He Y, Li M, Wujisiguleng, Lv B, Huan Y, Liu B, Wang D, Yu H, Zhang L and Shi Z: Zhenbao Pill reduces Treg cell proportion in acute spinal cord injury rats by regulating TUG1/miR-214/HSP27 axis. Biosci Rep. 38:BSR201808952018. View Article : Google Scholar : PubMed/NCBI | |
|
Shen Y, Xue CJ, You GL and Liu C: miR-9 alleviated the inflammatory response and apoptosis in caerulein-induced acute pancreatitis by regulating FGF10 and the NF-κB signaling pathway. Exp Ther Med. 22:7952021. View Article : Google Scholar : PubMed/NCBI | |
|
Zhang KK, Yu SS, Li GY, He L and Liang XQ: miR-135a deficiency inhibits the AR42J cells damage in cerulein-induced acute pancreatitis through targeting FAM129A. Pflugers Arch. 471:1519–1527. 2019. View Article : Google Scholar : PubMed/NCBI | |
|
Xu C, Luo Y, Ntim M, Quan W, Li Z, Xu Q, Jiang L, Zhang J, Shang D, Li L, et al: Effect of emodin on long non-coding RNA-mRNA networks in rats with severe acute pancreatitis-induced acute lung injury. J Cell Mol Med. 25:1851–1866. 2021. View Article : Google Scholar : PubMed/NCBI | |
|
Zhao JY, Wang JQ, Wu L, Zhang F, Chen ZP, Li WD, Cai H and Liu X: Emodin attenuates cell injury and inflammation in pancreatic acinar AR42J cells. J Asian Nat Prod Res. 21:186–195. 2019. View Article : Google Scholar : PubMed/NCBI | |
|
Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI | |
|
Guo Z, Wang X, Yang Y, Chen W, Zhang K, Teng B, Huang C, Zhao Q and Qiu Z: Hypoxic Tumor-derived exosomal long noncoding RNA UCA1 promotes angiogenesis via miR-96-5p/AMOTL2 in pancreatic cancer. Mol Ther Nucleic Acids. 22:179–195. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Jiang X, Li X, Zheng S, Du G, Ma J, Zhang L, Wang H and Tian J: Comparison study of different indoleamine-2,3 dioxygenase inhibitors from the perspective of pharmacodynamic effects. Int J Immunopathol Pharmacol. 34:20587384209505842020. View Article : Google Scholar : PubMed/NCBI | |
|
Fiori A, Uhlig S, Kluter H and Bieback K: Human adipose tissue-derived mesenchymal stromal cells inhibit CD4+ T cell proliferation and induce regulatory T cells as well as CD127 expression on CD4+CD25+ T cells. Cells. 10:582021. View Article : Google Scholar : PubMed/NCBI | |
|
Wang XD, Yu WL and Sun Y: Activation of AMPK restored impaired autophagy and inhibited inflammation reaction by up-regulating SIRT1 in acute pancreatitis. Life Sci. 277:1194352021. View Article : Google Scholar : PubMed/NCBI | |
|
Xiang H, Guo F, Tao X, Zhou Q, Xia S, Deng D, Li L and Shang D: Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation. Theranostics. 11:4467–4482. 2021. View Article : Google Scholar : PubMed/NCBI | |
|
Dai J, He Y, Jiang M, Niu M, Li B, Wu Z, Bao J, Wen L, Wang X and Hu G: Reg4 regulates pancreatic regeneration following pancreatitis via modulating the Notch signaling. J Cell Physiol. Apr 26–2021.(Epub ahead of print). View Article : Google Scholar | |
|
Lin HW, Lee YJ, Yang DJ, Hsieh MC, Chen CC, Hsu WL, Chang YY and Liu CW: Anti-inflammatory effects of flos lonicerae japonicae water extract are regulated by the STAT/NF-κB pathway and HO-1 expression in Virus-infected RAW264.7 cells. Int J Med Sci. 18:2285–2293. 2021. View Article : Google Scholar : PubMed/NCBI | |
|
Esmail MM, Saeed NM, Michel HE and El-Naga RN: The ameliorative effect of niclosamide on bile duct ligation induced liver fibrosis via suppression of NOTCH and Wnt pathways. Toxicol Lett. 347:23–35. 2021. View Article : Google Scholar : PubMed/NCBI | |
|
Xu C, Zhang J, Liu J, Li Z, Liu Z, Luo Y, Xu Q, Wang M, Zhang G, Wang F and Chen H: Proteomic analysis reveals the protective effects of emodin on severe acute pancreatitis induced lung injury by inhibiting neutrophil proteases activity. J Proteomics. 220:1037602020. View Article : Google Scholar : PubMed/NCBI | |
|
Xia S, Ni Y, Zhou Q, Liu H, Xiang H, Sui H and Shang D: Emodin attenuates severe acute pancreatitis via antioxidant and anti-inflammatory activity. Inflammation. 42:2129–2138. 2019. View Article : Google Scholar : PubMed/NCBI | |
|
Luo S, Deng X, Liu Q, Pan Z, Zhao Z, Zhou L and Luo X: Emodin ameliorates ulcerative colitis by the flagellin-TLR5 dependent pathway in mice. Int Immunopharmacol. 59:269–275. 2018. View Article : Google Scholar : PubMed/NCBI | |
|
Chen X and Song D: LncRNA MEG3 participates in caerulein-induced inflammatory injury in human pancreatic cells via regulating miR-195-5p/FGFR2 axis and inactivating NF-κB pathway. Inflammation. 44:160–173. 2021. View Article : Google Scholar : PubMed/NCBI | |
|
Tang Y, Hu J, Zhong Z, Liu Y and Wang Y: Long noncoding RNA TUG1 promotes the function in ox-LDL-treated HA-VSMCs via miR-141-3p/ROR2 axis. Cardiovasc Ther. 2020:67589342020. View Article : Google Scholar : PubMed/NCBI | |
|
Pei Y, Li K, Lou X, Wu Y, Dong X, Wang W, Li N, Zhang D and Cui W: miR1299/NOTCH3/TUG1 feedback loop contributes to the malignant proliferation of ovarian cancer. Oncol Rep. 44:438–448. 2020. View Article : Google Scholar : PubMed/NCBI | |
|
Zhang C, Zhang C, Xu Y, Li C, Cao Y and Li P: Exosomes derived from human placenta-derived mesenchymal stem cells improve neurologic function by promoting angiogenesis after spinal cord injury. Neurosci Lett. 739:1353992020. View Article : Google Scholar : PubMed/NCBI | |
|
Colucci M, Ruggiero B, Gianviti A, Rosado MM, Carsetti R, Bracaglia C, De Benedetti F, Emma F and Vivarelli M: IgM on the surface of T cells: A novel biomarker of pediatric-onset systemic lupus erythematosus. Pediatr Nephrol. 36:909–916. 2021. View Article : Google Scholar : PubMed/NCBI | |
|
Matikainen S, Nyman TA and Cypryk W: Inflammasomes: Exosomal miRNAs loaded for action. J Cell Biol. 219:e2020081302020. View Article : Google Scholar : PubMed/NCBI | |
|
Elashiry M, Elashiry MM, Elsayed R, Rajendran M, Auersvald C, Zeitoun R, Rashid MH, Ara R, Meghil MM, Liu Y, et al: Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivo. J Extracell Vesicles. 9:17953622020. View Article : Google Scholar : PubMed/NCBI | |
|
Wang N, Ma J, Ren Y, Xiang S and Jia R: Secreted klotho from exosomes alleviates inflammation and apoptosis in acute pancreatitis. Am J Transl Res. 11:3375–3383. 2019.PubMed/NCBI | |
|
Salminen A, Kaarniranta K and Kauppinen A: Exosomal vesicles enhance immunosuppression in chronic inflammation: Impact in cellular senescence and the aging process. Cell Signal. 75:1097712020. View Article : Google Scholar : PubMed/NCBI |
