Inhibitory effect of rice bran-derived crude glycosphingolipid on colon preneoplastic biomarker lesions induced by azoxymethane in male F344 rats

  • Authors:
    • Nao Sunagawa
    • Morihiko Inamine
    • Takamitsu Morioka
    • Itaru Chiba
    • Nanae Morita
    • Yoichi Aoki
    • Masumi Suzui
    • Naoki Yoshimi
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/mmr_00000060
  • Pages: 45-49
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Abstract

The aim of the present study was to examine whether crude glycosphingolipid (cGSL) has short-term chemopreventive effects on the preneoplastic biomarker lesions involved in carcinogen-induced rat colon carcinogenesis. We also examined whether cGSL affects cell proliferation and apoptosis in these lesions. The crude preparation was obtained by the simple ethanol extraction method. Five-week-old male F344 rats were divided into 6 groups. Rats in groups 1-4 were given subcutaneous injections of azoxymethane (AOM) (20 mg/kg body weight) once a week for 2 weeks. Starting 1 week before the first injection of AOM, the rats in groups 2, 3 and 4 were fed a diet containing 250, 1,000 and 3,000 ppm cGSL, respectively, for 5 weeks. The experiment was terminated 5 weeks after the start date, and the number of aberrant crypt foci (ACF) and mucin-depleted foci (MDF) was counted. Dietary cGSL significantly inhibited the induction of ACF (group 3, P<0.01; group 4, P<0.05) and MDF (groups 2 and 3, P<0.001; group 4, P<0.05) as compared to group 1 treated with AOM alone. In groups 3 and 4, proliferating cell nuclear antigen-positive indices of epithelial cells were significantly lower than in group 1 (group 3, P<0.05; group 4, P<0.005). Caspase-3-positive indices were significantly higher in groups 3 and 4 than in group 1 (group 3, P<0.01; group 4, P<0.001). These results suggest that dietary cGSL had a potent chemopreventive effect in the present short-term colon carcinogenesis bioassays, and that this effect may be associated with the inhibition of ACF and MDF and the induction of apoptosis.

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January-February 2009
Volume 2 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Sunagawa N, Inamine M, Morioka T, Chiba I, Morita N, Aoki Y, Suzui M and Yoshimi N: Inhibitory effect of rice bran-derived crude glycosphingolipid on colon preneoplastic biomarker lesions induced by azoxymethane in male F344 rats. Mol Med Rep 2: 45-49, 2009.
APA
Sunagawa, N., Inamine, M., Morioka, T., Chiba, I., Morita, N., Aoki, Y. ... Yoshimi, N. (2009). Inhibitory effect of rice bran-derived crude glycosphingolipid on colon preneoplastic biomarker lesions induced by azoxymethane in male F344 rats. Molecular Medicine Reports, 2, 45-49. https://doi.org/10.3892/mmr_00000060
MLA
Sunagawa, N., Inamine, M., Morioka, T., Chiba, I., Morita, N., Aoki, Y., Suzui, M., Yoshimi, N."Inhibitory effect of rice bran-derived crude glycosphingolipid on colon preneoplastic biomarker lesions induced by azoxymethane in male F344 rats". Molecular Medicine Reports 2.1 (2009): 45-49.
Chicago
Sunagawa, N., Inamine, M., Morioka, T., Chiba, I., Morita, N., Aoki, Y., Suzui, M., Yoshimi, N."Inhibitory effect of rice bran-derived crude glycosphingolipid on colon preneoplastic biomarker lesions induced by azoxymethane in male F344 rats". Molecular Medicine Reports 2, no. 1 (2009): 45-49. https://doi.org/10.3892/mmr_00000060