Antitumor activity of an adenovirus harboring two therapeutic genes, anti-VEGF ribozyme and human IL-24, in colon cancer

  • Authors:
    • Shujian Chang
    • Weichang Chen
    • Jicheng Yang
    • Yufeng Xie
    • Weihua Sheng
  • View Affiliations

  • Published online on: September 1, 2009     https://doi.org/10.3892/mmr_00000158
  • Pages: 693-700
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Increased vascular endothelial growth factor (VEGF) expression and tumor vascularization are correlated with both tumor progression and a poor clinical prognosis in colon cancer. There is increasing evidence that mda-7/IL-24 has potent growth suppression activity and induces apoptosis in many different tumor cells. We constructed a recombinant replication-deficient adenovirus carrying both an anti-VEGF hairpin ribozyme and human IL-24 (Ad-Rz/IL-24) with an internal ribosome entry site and tested its VEGF inhibition effect and antitumor activity. The results showed that Ad-Rz/IL-24 significantly inhibited VEGF expression and angiogenesis in vitro and in vivo. The proliferation rate of xenograft tumors treated with Ad-Rz/IL-24 was approximately 50% lower than the rate of the xenograft tumors treated with adenovirus carrying a single gene anti-VEGF hairpin ribozyme (Ad-Rz) or IL-24 (Ad-IL-24) alone. These findings demonstrated the potential therapeutic effect of this dual gene-expressing adenovirus for cancer gene therapy.

Related Articles

Journal Cover

September-October 2009
Volume 2 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chang S, Chen W, Yang J, Xie Y and Sheng W: Antitumor activity of an adenovirus harboring two therapeutic genes, anti-VEGF ribozyme and human IL-24, in colon cancer. Mol Med Rep 2: 693-700, 2009.
APA
Chang, S., Chen, W., Yang, J., Xie, Y., & Sheng, W. (2009). Antitumor activity of an adenovirus harboring two therapeutic genes, anti-VEGF ribozyme and human IL-24, in colon cancer. Molecular Medicine Reports, 2, 693-700. https://doi.org/10.3892/mmr_00000158
MLA
Chang, S., Chen, W., Yang, J., Xie, Y., Sheng, W."Antitumor activity of an adenovirus harboring two therapeutic genes, anti-VEGF ribozyme and human IL-24, in colon cancer". Molecular Medicine Reports 2.5 (2009): 693-700.
Chicago
Chang, S., Chen, W., Yang, J., Xie, Y., Sheng, W."Antitumor activity of an adenovirus harboring two therapeutic genes, anti-VEGF ribozyme and human IL-24, in colon cancer". Molecular Medicine Reports 2, no. 5 (2009): 693-700. https://doi.org/10.3892/mmr_00000158