Inhibition of MCF-7 breast cancer cell-induced platelet aggregation using a combination of antiplatelet drugs

Lian,Lian; Li,Wei; Li,Zhen-Yu; Mao,Yi-Xiang; Zhang,You-Tao; Zhao,Yi-Ming; Chen,Kai; Duan,Wei-Ming; Tao,Min

doi:10.3892/ol.2012.1074

Inhibition of MCF-7 breast cancer cell-induced platelet aggregation using a combination of antiplatelet drugs

Authors:
- Lian Lian
- Wei Li
- Zhen-Yu Li
- Yi-Xiang Mao
- You-Tao Zhang
- Yi-Ming Zhao
- Kai Chen
- Wei-Ming Duan
- Min Tao
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Affiliations: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, P.R. China, Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536-0200, USA, Department of Clinical Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, P.R. China, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, P.R. China
Published online on: December 13, 2012 https://doi.org/10.3892/ol.2012.1074
Pages: 675-680

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Abstract

Cancer metastasis is a highly coordinated and dynamic multistep process in which cancer cells interact with a variety of host cells. Morphological studies have documented the association of circulating tumor cells with host platelets. Tumor cell-induced platelet aggregation (TCIPA) contributes significantly to hematogenous metastasis; however, the molecular mechanisms involved in breast cancer TCIPA are poorly characterized. In this study, MCF-7 metastatic human breast cancer cells induced dose-dependent aggregation of washed platelets. Four major platelet activation pathways, glycoprotein (GP)-Ib-IX, GPIIb/IIIa, thromboxane (TX)-A2 and adenosine diphosphate (ADP) were activated during TCIPA and were inhibited by their respective inhibitors, 7E3, SZ-1, aspirin and apyrase. Pretreatment of platelets with 7E3, SZ-1 or apyrase significantly inhibited TCIPA, while pretreatment with aspirin had no effect. Moreover, combined pretreatment of platelets with 7E3, SZ-1 and apyrase significantly inhibited TCIPA, compared to single inhibitors. Combinations of antiplatelet drugs may represent a promising strategy to prevent cancer metastasis.

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