Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells

  • Authors:
    • Yuting Yao
    • Lu Li
    • He Zhang
    • Renbing Jia
    • Bo Liu
    • Xiaoping Zhao
    • Leilei Zhang
    • Guanxiang Qian
    • Xianqun Fan
    • Shengfang Ge
  • View Affiliations

  • Published online on: April 17, 2012     https://doi.org/10.3892/ol.2012.682
  • Pages: 163-167
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Abstract

Vitamin K2 (VK2) exerts cell growth inhibitory effects in various human cancer cells such as SMMC-7721 hepatocellular carcinoma (HCC) cells. BCL-2 is an antiapoptotic protein that is frequently overexpressed in numerous tumors. Modulation of multiple antiapoptotic signaling pathways involving BCL-2, which are related to growth factor-stimulated signal transduction in cell survival, is essential for enhancement of the cytotoxic effect of anticancer drugs. In this study, we tested a new strategy of gene therapy by combining BCL-2 siRNA with VK2. In SMMC-7721 HCC cells, the combined treatment significantly enhanced cytotoxicity compared with treatment with either VK2 or siBCL-2 alone. We found that combined treatment induced a significantly different level of G2 stage inhibition. Furthermore, the p53 protein was overexpressed 24 h subsequent to combination treatment, and p21 was clearly increased at 36 h as a consequence of the increased p53 activity. In conclusion, these data suggest that the antitumor effect of VK2 may be improved by silencing BCL-2 expression in SMMC-7721 HCC cells and provides support for the combined use of VK2 and siBCL-2 as a promising approach in cancer gene therapy.
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July 2012
Volume 4 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yao Y, Li L, Zhang H, Jia R, Liu B, Zhao X, Zhang L, Qian G, Fan X, Ge S, Ge S, et al: Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells. Oncol Lett 4: 163-167, 2012.
APA
Yao, Y., Li, L., Zhang, H., Jia, R., Liu, B., Zhao, X. ... Ge, S. (2012). Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells. Oncology Letters, 4, 163-167. https://doi.org/10.3892/ol.2012.682
MLA
Yao, Y., Li, L., Zhang, H., Jia, R., Liu, B., Zhao, X., Zhang, L., Qian, G., Fan, X., Ge, S."Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells". Oncology Letters 4.1 (2012): 163-167.
Chicago
Yao, Y., Li, L., Zhang, H., Jia, R., Liu, B., Zhao, X., Zhang, L., Qian, G., Fan, X., Ge, S."Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells". Oncology Letters 4, no. 1 (2012): 163-167. https://doi.org/10.3892/ol.2012.682