Casein induces the proliferation of bone marrow mononuclear cells, apoptosis of WEHI-3 leukaemic cells and increased survival in a leukaemia mouse model

Ledesma-Martínez,E.; Pérez-Cordero,C.; Córdova-Galaviz,Y.; Sánchez-Tellez,G.; Huerta-Yepez,S.; Aguiñiga-Sánchez,I.; Miranda-Peralta,E.; Monroy-García,A.; Weiss-Steider,B.; Santiago-Osorio,E.

doi:10.3892/ol.2012.758

Casein induces the proliferation of bone marrow mononuclear cells, apoptosis of WEHI-3 leukaemic cells and increased survival in a leukaemia mouse model

Authors:
- E. Ledesma-Martínez
- C. Pérez-Cordero
- Y. Córdova-Galaviz
- G. Sánchez-Tellez
- S. Huerta-Yepez
- I. Aguiñiga-Sánchez
- E. Miranda-Peralta
- A. Monroy-García
- B. Weiss-Steider
- E. Santiago-Osorio
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Affiliations: Hematopoiesis and Leukaemia Laboratory, Research Unit on Cell Differentiation and Cancer, National Autonomous University of Mexico, Mexico City, Mexico, Research Unit in Oncological Diseases, Childhood Hospital of Mexico “Federico Gómez”, Mexico City, Mexico, Cancer Research Unit, Oncology Hospital, Mexico City, Mexico, Immunology and Cancer Laboratory, Medical Research Unit on Oncological Diseases, Mexican Social Security Institute (IMSS), Mexico City, Mexico
Published online on: June 14, 2012 https://doi.org/10.3892/ol.2012.758
Pages: 461-466

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Abstract

Acute myeloid leukaemia results from the neoplastic transformation of haematopoietic stem cells. Although advances have been made in its treatment, the mortality rate remains high. As a result, therapeutic alternatives continue to be explored. In this study, we present evidence that suggests that casein, the principal protein in milk, possesses significant antileukaemic properties. We investigated whether casein inhibited the in vitro proliferation and induced the apoptosis of the mouse myelomonocytic leukaemia cell line WEHI-3. By contrast, under identical conditions, casein markedly promotes the proliferation of mouse normal mononuclear bone marrow cells. Since the selective elimination of leukaemia cells is an ideal therapeutic strategy, we also evaluated the antileukaemic potential of casein in vivo. The results showed that casein increases the survival of mice bearing WEHI-3-induced tumours, suggesting that this molecule is also capable of inhibiting the proliferation of these cells in vivo. The evidence that casein inhibited cell proliferation and induced apoptosis in leukaemia cells in vitro, but increased survival in vivo in a leukaemia mouse model, indicates that casein may be useful in leukaemia therapy.

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