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Abnormal methylation of seven genes and their associations with clinical characteristics in early stage non-small cell lung cancer

  • Authors:
    • Yangxing Zhao
    • Huafu Zhou
    • Kelong Ma
    • Jinfeng Sun
    • Xu Feng
    • Junfeng Geng
    • Jun Gu
    • Wei Wang
    • Hongyu Zhang
    • Yinghua He
    • Shicheng Guo
    • Xiaoyu Zhou
    • Jian Yu
    • Qiang Lin

  • View Affiliations

  • Published online on: January 29, 2013     https://doi.org/10.3892/ol.2013.1161
  • Pages: 1211-1218
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Abstract

To identify novel abnormally methylated genes in early stage non-small cell lung cancer (NSCLC), we analyzed the methylation status of 13 genes (ALX1, BCL2, FOXL2, HPP1, MYF6, OC2, PDGFRA, PHOX2A, PITX2, RARB, SIX6, SMPD3 and SOX1) in cancer tissues from 101 cases of stage I NSCLC patients and lung tissues from 30 cases of non-cancerous lung disease controls, using methylation-specific PCR (MSP). The methylation frequencies (29.70-64.36%) of 7 genes (MYF6, SIX6, SOX1, RARB, BCL2, PHOX2A and FOLX2) in stage I NSCLC were significantly higher compared with those in non-cancerous lung disease controls (P<0.05). The co-methylation of SIX6 and SOX1, or the co-methyaltion of SIX6, RARB and SOX1 was associated with adenosquamous carcinoma (ADC), and the co-methylation of BCL2, RARB and SIX6 was associated with smoking. A panel of 4 genes (MYF6, SIX6, BCL2 and RARB) may offer a sensitivity of 93.07% and a specificity of 83.33% in the diagnosis of stage I NSCLC. Furthermore, we also detected the expression of 8 pathological markers (VEGF, HER-2, P53, P21, EGFR, CHGA, SYN and EMA) in cancer tissues of stage I NSCLC by immunohistochemistry, and found that high expression levels of p53 and CHGA were associated with the methylation of BCL2 (P=0.025) and PHOX2A (P=0.023), respectively. In this study, among the 7 genes which demonstrated hypermethylation in stage I NSCLC compared with non-cancerous lung diseases, 5 genes (MYF6, SIX6, PHOX2A, FOLX2 and SOX1) were found for the first time to be abonormally methylated in NSCLC. Further study of these genes shed light on the carcinogenesis of NSCLC.
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April 2013
Volume 5 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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  • Impact Factor: 2.5
  • Ranked Oncology
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  • CiteScore: 5.7
  • CiteScore Rank: Q2: #139/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.574
  • SCImago Journal Rank (SJR): 0.644
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Spandidos Publications style
Zhao Y, Zhou H, Ma K, Sun J, Feng X, Geng J, Gu J, Wang W, Zhang H, He Y, He Y, et al: Abnormal methylation of seven genes and their associations with clinical characteristics in early stage non-small cell lung cancer. Oncol Lett 5: 1211-1218, 2013.
APA
Zhao, Y., Zhou, H., Ma, K., Sun, J., Feng, X., Geng, J. ... Lin, Q. (2013). Abnormal methylation of seven genes and their associations with clinical characteristics in early stage non-small cell lung cancer. Oncology Letters, 5, 1211-1218. https://doi.org/10.3892/ol.2013.1161
MLA
Zhao, Y., Zhou, H., Ma, K., Sun, J., Feng, X., Geng, J., Gu, J., Wang, W., Zhang, H., He, Y., Guo, S., Zhou, X., Yu, J., Lin, Q."Abnormal methylation of seven genes and their associations with clinical characteristics in early stage non-small cell lung cancer". Oncology Letters 5.4 (2013): 1211-1218.
Chicago
Zhao, Y., Zhou, H., Ma, K., Sun, J., Feng, X., Geng, J., Gu, J., Wang, W., Zhang, H., He, Y., Guo, S., Zhou, X., Yu, J., Lin, Q."Abnormal methylation of seven genes and their associations with clinical characteristics in early stage non-small cell lung cancer". Oncology Letters 5, no. 4 (2013): 1211-1218. https://doi.org/10.3892/ol.2013.1161