Honokiol‑induced apoptosis and autophagy in glioblastoma multiforme cells

Chang,Ken‑Hu; Yan,Ming‑De; Yao,Chih‑Jung; Lin,Pei‑Chun; Lai,Gi‑Ming

doi:10.3892/ol.2013.1548

Honokiol‑induced apoptosis and autophagy in glioblastoma multiforme cells

Authors:
- Ken‑Hu Chang
- Ming‑De Yan
- Chih‑Jung Yao
- Pei‑Chun Lin
- Gi‑Ming Lai
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Affiliations: Division of Hematology Oncology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, Taipei 116, Taiwan, R.O.C., Division of Gastroenterology, Department of Internal Medicine, Wang Fan Hospital, Taipei Medical University, Taipei 116, Taiwan, R.O.C., Center of Excellence for Cancer Research, Taipei Medical University, Taipei 116, Taiwan, R.O.C.
Published online on: August 28, 2013 https://doi.org/10.3892/ol.2013.1548
Pages: 1435-1438

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Abstract

Honokiol, a hydroxylated biphenyl compound isolated from the Chinese herb Magnolia officinalis, has been reported to have anticancer activities in a variety of cancer cell lines. The present study aimed to evaluate the anticancer effect and possible molecular mechanisms of honokiol in a glioblastoma multiforme (GBM) cell line. The anticancer activities of honokiol were investigated in the DBTRG‑05MG GBM cell line. The effect of honokiol on cell growth was determined using a sulforhodamine B assay. Flow cytometry and immunoblotting were used to measure honokiol‑induced apoptosis (programmed cell death type I) and autophagy (programmed cell death type II). Honokiol was observed to reduce DBTRG‑05MG cell viability in a dose‑dependent manner. At a dose of 50 µM, honokiol markedly decreased the expression of Rb protein and led to the cleavage of poly(ADP‑ribose) polymerase and Bcl‑xL to promote apoptosis in the cancer cells. In addition, markers of autophagy, including Beclin‑1 and LC3‑II, were also significantly increased. In addition to apoptosis, honokiol was also able to induce autophagy in the DBTRG‑05MG cells. The mechanisms that are responsible for the correlation between honokiol‑induced apoptosis and autophagy require further investigation. Such efforts may provide a potential strategy for improving the clinical outcome of GBM treatment.

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