Open Access

Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells

  • Authors:
    • Lin‑Lin Yan
    • Yuan‑Jiao Huang
    • Xiang Yi
    • Xue‑Min Yan
    • Yan Cai
    • Qin He
    • Zi‑Jian Han
  • View Affiliations

  • Published online on: March 31, 2015     https://doi.org/10.3892/ol.2015.3090
  • Pages: 2534-2540
  • Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The calcium-binding S100 proteins are involved in functions such as cell growth, differentiation, migration, adhesion and signal transduction. S100A8 and S100A9 are highly expressed in a variety of tumor cells, and are implicated in tumor development and progression. However, the role of S100A8 and S100A9 in nasopharyngeal carcinoma (NPC) cell migration is unclear. The present study investigated the effect of S100A8 and S100A9 on migration using a NPC cell line, CNE1. The CNE1 cells were transfected with S100A8 or S100A9 small interfering RNA (siRNA). Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect S100A8 and S100A9 gene expression. Following the downregulation of S100A8 or S100A9, the effects on cell migration were determined using wound‑healing assays. The expression of matrix metalloproteinase‑7 (MMP7), a member of the MMP family that is associated with tumor cell invasion and migration, was also detected by RT‑qPCR. S100A8 and S100A9 siRNAs effectively suppressed S100A8 and S100A9 gene expression, and substantially inhibited the migration of the CNE1 cells. In addition, MMP7 expression was reduced to varying extents in S100A8 and S100A9 siRNA-treated cells compared with controls. Thus, S100A8 and S100A9 promoted the migration of CNE1 NPC cells.
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June-2015
Volume 9 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yan LL, Huang YJ, Yi X, Yan XM, Cai Y, He Q and Han ZJ: Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells. Oncol Lett 9: 2534-2540, 2015.
APA
Yan, L., Huang, Y., Yi, X., Yan, X., Cai, Y., He, Q., & Han, Z. (2015). Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells. Oncology Letters, 9, 2534-2540. https://doi.org/10.3892/ol.2015.3090
MLA
Yan, L., Huang, Y., Yi, X., Yan, X., Cai, Y., He, Q., Han, Z."Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells". Oncology Letters 9.6 (2015): 2534-2540.
Chicago
Yan, L., Huang, Y., Yi, X., Yan, X., Cai, Y., He, Q., Han, Z."Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells". Oncology Letters 9, no. 6 (2015): 2534-2540. https://doi.org/10.3892/ol.2015.3090