Caspase-dependent drug-induced apoptosis is regulated by cell surface sialylation in human B-cell lymphoma

Suzuki,Osamu; Abe,Masafumi; Hashimoto,Yuko

doi:10.3892/ol.2015.3320

Caspase-dependent drug-induced apoptosis is regulated by cell surface sialylation in human B-cell lymphoma

Authors:
- Osamu Suzuki
- Masafumi Abe
- Yuko Hashimoto
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Affiliations: Department of Diagnostic Pathology, School of Medicine, Fukushima Medical University, Fukushima 960‑1295, Japan
Published online on: June 4, 2015 https://doi.org/10.3892/ol.2015.3320
Pages: 687-690

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Abstract

The important role of sialic acid in various biological phenomena is well-established. In order to further clarify the role of sialic acid in cell death induced by various stimuli, the present study compared the cell survival of the HBL‑2 human diffuse large B‑cell lymphoma cell line upon anticancer drug‑induced cell death, with or without neuraminidase pretreatment: Cell survival was assessed using flow cytometry. Upon treatment with doxorubicin or etoposide, the HBL‑2 cell viability decreased. In etoposide‑induced cell death, the HBL‑2 cells demonstrated nuclear fragmentation, which was consistent with morphologically apoptotic cells. In addition, a higher decrease in the cell viability of etoposide‑treated HBL‑2 cells was observed in cells pretreated with neuraminidase compared with cells that were not pretreated. Furthermore, the caspase‑3, caspase‑8 and caspase‑9 activities in etoposide‑induced apoptosis demonstrated a greater increase upon neuraminidase pretreatment compared with no neuraminidase pretreatment. In conclusion, cell surface sialylation appears to protect lymphoma cells from anticancer drug-induced apoptosis.

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