Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells

Lee,Ju‑Hee; Park,Chul  Hong; Chun,Kwang‑Hoon; Hong,Soon‑Sun

doi:10.3892/ol.2015.3360

Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells

Authors:
- Ju‑Hee Lee
- Chul Hong Park
- Kwang‑Hoon Chun
- Soon‑Sun Hong
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Affiliations: Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 400‑712, Republic of Korea, Gachon Institute of Pharmaceutical Sciences, College of Pharmacy, Gachon University, Incheon 406‑799, Republic of Korea
Published online on: June 10, 2015 https://doi.org/10.3892/ol.2015.3360
Pages: 730-736
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Adipose-derived stem cells (ASCs) are a population of cells derived from adipose tissue. ASCs exhibit multilineage development potential and are able to secrete various factors, which influence adjacent cells. Previous studies have reported the effectiveness of ASC‑conditioned medium (ASC‑CM) in wound healing, anti‑melanogenesis, wrinkle improvement and hair growth. In the present study, the anticancer function of ASC‑CM was investigated in vitro and in vivo. An MTT assay revealed that ASC‑CM significantly decreased the proliferation of B16 melanoma cells in a time‑ and dose‑dependent manner (P<0.01). Cell cycle analysis indicated that ASC‑CM significantly increased the number of cells in G1 phase while reducing the number of cells in the S and G2/M phases (P<0.01). Furthermore, a wound migration model demonstrated that ASC‑CM treatment significantly decreased the migration ability of B16 melanoma cells (P<0.01). In addition, C57BL/6 mice were administered with a single intratumoral injection of ASC‑CM, daily or every other day, and a significant reduction in the volume of the tumor mass was observed compared with that of the control group (P<0.01). Thus, the findings of the present study indicated that ASC‑CM has an anti‑tumorigenic effect on B16 melanoma cells in vitro and in vivo, and may potentially be used to support the treatment of melanoma in the future.

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