MicroRNA heterogeneity in endometrial cancer cell lines revealed by deep sequencing

Lu,Jiafeng; Zhang,Xueli; Zhang,Rong; Ge,Qinyu

doi:10.3892/ol.2015.3776

MicroRNA heterogeneity in endometrial cancer cell lines revealed by deep sequencing

Authors:
- Jiafeng Lu
- Xueli Zhang
- Rong Zhang
- Qinyu Ge
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Affiliations: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu 210096, P.R. China, Department of Surgery, Fengxian Central Hospital, Shanghai 201400, P.R. China, Department of Obstetrics and Gynecology, Fengxian Central Hospital, Shanghai 201400, P.R. China
Published online on: October 2, 2015 https://doi.org/10.3892/ol.2015.3776
Pages: 3457-3465
Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to obtain comprehensive microRNA (miRNA) profiles of type I [Ishikawa (ISK)] and type II (HEC-1B) human endometrial adenocarcinoma cell lines, utilizing the latest high‑throughput sequencing techniques. RNA was extracted from ISK and HEC‑1B cell lines. Sequencing results were obtained from a next-generation sequencing platform. Using the miRBase database and a series of software pipelines, miRNA expression was analyzed in the ISK and HEC‑1B cell lines. It was revealed that the type and quantity of miRNAs in the two cell types varied significantly; 34 miRNAs were upregulated and 105 miRNAs were downregulated in HEC‑1B cells compared with those of ISK cells. Furthermore, it was observed that the expression pattern of the miRNA (miR)‑17‑92 cluster differed between the two cell types, and the expression levels of the miR‑200 family in ISK cells were markedly increased compared with those of HEC‑1B cells. The present study therefore identified potential novel biomarkers, which may be useful in the differentiation between type I and type II endometrial cancer, and also revealed miRNA alterations that may be associated with endometrial cancer and its underlying pathogenic mechanisms.

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