Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors
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- Published online on: January 11, 2016 https://doi.org/10.3892/ol.2016.4099
- Pages: 1299-1304
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Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
Wilms' tumors are one of the most common malignant, solid intra-abdominal tumors observed in children. Although potential tumor markers have been found, inflammatory cytokines interfere with the process of specific protein identification. The present study was undertaken to identify post-traumatic stress-related factors of Wilms' tumors and to verify the accuracy of early‑stage tumor‑specific serum protein markers. Serum samples were screened for differentially-expressed proteins using surface‑enhanced laser desorption/ionization‑time of flight mass spectrometry (SELDI‑TOF‑MS). Potential markers were isolated and purified using solid‑phase extraction (SPE) and SDS‑PAGE. Following enzymatic digestion of the protein samples, the peptide fragments were detected with high performance liquid chromatography‑mass spectrometry. The obtained peptide mass fingerprint was searched in the Swiss‑Prot protein sequence database via the Mascot search engine. Differentially‑expressed proteins were verified using western blot analysis. Differentially‑expressed proteins with a mass/charge of 5,816 were screened out using SELDI‑TOF‑MS, and significant differences between the tumor and control groups, and the trauma and control groups were observed. Target proteins were isolated and purified using SPE and SDS‑PAGE. Thioredoxin 1 (Trx1) was found to be differentially expressed. In the serum of children with Wilms' tumors, there was an increase in the level of the post‑traumatic stress‑related inflammatory factor, Trx1, as compared with the normal control group. Thus, the results of this study indicate that Trx1 presents a potential post‑traumatic stress-related factor of Wilms' tumors.
