Open Access

Lipid raft localization of epidermal growth factor receptor alters matrix metalloproteinase-1 expression in SiHa cells via the MAPK/ERK signaling pathway

  • Authors:
    • Zongfeng Zhang
    • Lina Wang
    • Juan Du
    • Yuanbo Li
    • Huilun Yang
    • Chenxi Li
    • Hui Li
    • Haiyang Hu
  • View Affiliations

  • Published online on: October 24, 2016     https://doi.org/10.3892/ol.2016.5307
  • Pages: 4991-4998
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Matrix metalloproteinase-1 (MMP-1) has been identified as an important participant in tumor invasion, metastasis and angiogenesis. The purpose of the present study was to investigate the effects of epidermal growth factor receptor (EGFR) localization to lipid rafts on signaling pathways involved in the regulation of MMP‑1 expression in SiHa cells, a cervical cancer cell line. EGFR activation by EGF specifically induced MMP‑1 expression at both the messenger RNA and protein levels. Additionally, it was observed that EGFR localized to lipid rafts, and that the redistribution of EGFR induced by lipid raft disruption strengthened EGF‑induced MMP‑1 expression. MMP‑1 induction was blocked by the mitogen‑activated protein kinase (MAPK) kinase inhibitors PD98059 and U0126. Our results suggested that lipid rafts provide a platform to inhibit EGFR regulation of MMP‑1 in SiHa cells through the MAPK/extracellular signal-regulated kinase signaling pathway.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang Z, Wang L, Du J, Li Y, Yang H, Li C, Li H and Hu H: Lipid raft localization of epidermal growth factor receptor alters matrix metalloproteinase-1 expression in SiHa cells via the MAPK/ERK signaling pathway. Oncol Lett 12: 4991-4998, 2016.
APA
Zhang, Z., Wang, L., Du, J., Li, Y., Yang, H., Li, C. ... Hu, H. (2016). Lipid raft localization of epidermal growth factor receptor alters matrix metalloproteinase-1 expression in SiHa cells via the MAPK/ERK signaling pathway. Oncology Letters, 12, 4991-4998. https://doi.org/10.3892/ol.2016.5307
MLA
Zhang, Z., Wang, L., Du, J., Li, Y., Yang, H., Li, C., Li, H., Hu, H."Lipid raft localization of epidermal growth factor receptor alters matrix metalloproteinase-1 expression in SiHa cells via the MAPK/ERK signaling pathway". Oncology Letters 12.6 (2016): 4991-4998.
Chicago
Zhang, Z., Wang, L., Du, J., Li, Y., Yang, H., Li, C., Li, H., Hu, H."Lipid raft localization of epidermal growth factor receptor alters matrix metalloproteinase-1 expression in SiHa cells via the MAPK/ERK signaling pathway". Oncology Letters 12, no. 6 (2016): 4991-4998. https://doi.org/10.3892/ol.2016.5307