A subpopulation of cancer stem cells identifies radiographic characteristics in glioblastoma

Kim,Ja  Eun; Kim,Sung  Kwon; Shin,Jaekyung; Se,Young‑Bem; Choi,Seung  Hong; Park,Sung‑Hye; Choi,Seung  Ah; Lee,Ji  Yeoun; Phi,Ji  Hoon; Wang,Kyu‑Chang; Park,Chul‑Kee; Kim,Seung‑Ki

doi:10.3892/ol.2016.5548

A subpopulation of cancer stem cells identifies radiographic characteristics in glioblastoma

Authors:
- Ja Eun Kim
- Sung Kwon Kim
- Jaekyung Shin
- Young‑Bem Se
- Seung Hong Choi
- Sung‑Hye Park
- Seung Ah Choi
- Ji Yeoun Lee
- Ji Hoon Phi
- Kyu‑Chang Wang
- Chul‑Kee Park
- Seung‑Ki Kim
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Affiliations: Department of Neurosurgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea, Department of Neurosurgery, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Jinju 660‑702, Republic of Korea, Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea, Department of Pathology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea, Department of Anatomy, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea
Published online on: December 28, 2016 https://doi.org/10.3892/ol.2016.5548
Pages: 1175-1182
Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cancer stem cells (CSCs), defined by CD133 expression, harbor heterogeneous subpopulations of cells, including endothelial progenitor cells (EPCs). This study aimed to investigate whether a subpopulation of CSCs could affect the radiographic characteristics of glioblastoma. Tissue samples from 10 patients newly diagnosed with glioblastoma were selected according to the radiographic characteristics of their tumors. The patients were divided into two groups based on preoperative magnetic resonance imaging demonstrating contrast enhancement, necrosis and infiltrative patterns: the enhancement/necrosis group (E/N, n=5) and the non‑enhancement/infiltration group (NE/I, n=5). Flow cytometry was used to assess the CSCs while immunohistochemistry was used to study microvessel density and the proliferation index. The EPC (CD34+/CD133+) fraction in CSCs (CD133+) was larger in the NE/I group. However, there was little difference in the angiogenic activity assessed using microvessel density between the two groups. The proliferation index (assessed using the antibody Ki‑67) was higher in the E/N group and was negatively correlated with the EPC fraction. The non‑EPC (CD34‑/CD133+) fraction is a major factor responsible for radiographic characteristics of contrast enhancement, thus establishing an association between a subpopulation fraction of CSCs and radiographic characteristics in glioblastoma. Therefore, the simple non‑invasive assessment of studying contrast enhancement lesions in glioblastomas may be used to estimate CSC subpopulations.

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