Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma

  • Authors:
    • Benedikt Kramer
    • Johannes David Schultz
    • Clemens Hock
    • Alexander Sauter
    • Boris A. Stuck
    • Karl Hörmann
    • Richard Birk
    • Christoph Aderhold
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  • Published online on: March 13, 2017     https://doi.org/10.3892/ol.2017.5844
  • Pages: 3269-3276
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Abstract

The identification of molecular targets in the therapy of human papilloma virus (HPV)‑associated head and neck squamous cell carcinoma (HNSCC) is a primary aim of cancer research. Matrix metalloproteinase 9 (MMP-9) and vascular endothelial growth factor receptor (VEGFR) have important roles in the development of HNSCC. The tyrosine kinase inhibitors, nilotinib, dasatinib, erlotinib and gefitinib are well established in the targeted therapy of tumors other than HNSCC. The present study aimed to investigate the alteration of MMP‑9 and VEGFR‑1 expression patterns following treatment with these tyrosine kinase inhibitors in p16‑positive and ‑negative squamous carcinoma cells. MMP‑9 and VEGFR‑1 expression was evaluated using an ELISA in HNSCC 11A, HNSCC 14C and p16‑positive CERV196 tumor cell lines, following treatment with nilotinib, dasatinib, erlotinib and gefitinib. A statistically significant reduction in MMP‑9 and VEGFR‑1 expression was observed in the p16‑negative HNSCC 11A cells following treatment with all inhibitors (P<0.05). VEGFR‑1 expression was significantly increased in p16‑positive SCC cells following treatment with nilotinib, dasatinib, erlotinib and gefitinib (P<0.05). The expression of MMP‑9 and VEGFR‑1 was significantly altered by treatment with nilotinib, dasatinib, erlotinib and gefitinib in vitro. The results of the present study are attributed to the efficacy of the tested drugs and present potential compensatory strategies of cancer cells to avoid the antiangiogenic properties of the tested tyrosine kinase inhibitors in vitro.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Kramer B, Schultz JD, Hock C, Sauter A, Stuck BA, Hörmann K, Birk R and Aderhold C: Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma. Oncol Lett 13: 3269-3276, 2017.
APA
Kramer, B., Schultz, J.D., Hock, C., Sauter, A., Stuck, B.A., Hörmann, K. ... Aderhold, C. (2017). Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma. Oncology Letters, 13, 3269-3276. https://doi.org/10.3892/ol.2017.5844
MLA
Kramer, B., Schultz, J. D., Hock, C., Sauter, A., Stuck, B. A., Hörmann, K., Birk, R., Aderhold, C."Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma". Oncology Letters 13.5 (2017): 3269-3276.
Chicago
Kramer, B., Schultz, J. D., Hock, C., Sauter, A., Stuck, B. A., Hörmann, K., Birk, R., Aderhold, C."Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma". Oncology Letters 13, no. 5 (2017): 3269-3276. https://doi.org/10.3892/ol.2017.5844