Monoclonal antibodies against autocrine motility factor suppress gastric cancer

  • Authors:
    • Hahn‑Sun Jung
    • Su In Lee
    • Seung‑Hoon Kang
    • Jin Sang Wang
    • Eun Hee Yang
    • Byungwook Jeon
    • Jayhyuk Myung
    • Ji Young Baek
    • Song‑Kyu Park
  • View Affiliations

  • Published online on: April 13, 2017     https://doi.org/10.3892/ol.2017.6037
  • Pages: 4925-4932
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Abstract

Autocrine motility factor (AMF), which is a secreted form of phosphoglucose isomerase, is mainly secreted by various tumors and has cytokine‑like activity. AMF is known to stimulate proliferation, survival and metastasis of cancer cells, and angiogenesis within a tumor. The present study investigated whether inhibition of AMF using targeted-antibodies was able to suppress the growth of cancer. A migration assay using a Boyden chamber was utilized to measure the activity of AMF on the motility of cancer cells. A recombinant human AMF (rhAMF) prepared from E. coli transformed with the pET22b‑AMF vector increased the motility of MDA‑MB‑231 and A549 cells, but it did not affect that of NCI‑N87 or HepG2 cells, which exhibited the ability to secrete high amounts of their own endogenous AMF into the culture medium. The extent to which the AMF receptor was expressed on cancer cells did not correlate clearly with the cell motility stimulated by rhAMF. In A549‑xenografted nude mice treated with sunitinib or cetuximab, a decrease in the plasma AMF concentration was accompanied by a reduction in tumor weight, suggesting an association between the plasma AMF concentration and anticancer activity. A monoclonal antibody (9A‑4H), which revealed a high binding affinity for E. coli-derived rhAMF, significantly suppressed the growth of tumors in Balb/c nude mice transplanted with the human gastric cancer cell line NCI‑N87, to the similar extent as trastuzumab, an anticancer antibody. The present study suggests, for the first time, that an antibody specific to AMF may be a therapeutic agent for gastric cancer.
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June-2017
Volume 13 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Jung HS, Lee SI, Kang SH, Wang JS, Yang EH, Jeon B, Myung J, Baek JY and Park SK: Monoclonal antibodies against autocrine motility factor suppress gastric cancer. Oncol Lett 13: 4925-4932, 2017
APA
Jung, H., Lee, S.I., Kang, S., Wang, J.S., Yang, E.H., Jeon, B. ... Park, S. (2017). Monoclonal antibodies against autocrine motility factor suppress gastric cancer. Oncology Letters, 13, 4925-4932. https://doi.org/10.3892/ol.2017.6037
MLA
Jung, H., Lee, S. I., Kang, S., Wang, J. S., Yang, E. H., Jeon, B., Myung, J., Baek, J. Y., Park, S."Monoclonal antibodies against autocrine motility factor suppress gastric cancer". Oncology Letters 13.6 (2017): 4925-4932.
Chicago
Jung, H., Lee, S. I., Kang, S., Wang, J. S., Yang, E. H., Jeon, B., Myung, J., Baek, J. Y., Park, S."Monoclonal antibodies against autocrine motility factor suppress gastric cancer". Oncology Letters 13, no. 6 (2017): 4925-4932. https://doi.org/10.3892/ol.2017.6037