miR‑200b inhibits CD133+ glioma cells by targeting the AKT pathway

Liu,Aiqun; Yu,Qingyun; Peng,Zhongxing; Huang,Yeqing; Diao,Shengpeng; Cheng,Jing; Wang,Wentao; Hong,Mingfan

doi:10.3892/ol.2017.6055

miR‑200b inhibits CD133+ glioma cells by targeting the AKT pathway

Authors:
- Aiqun Liu
- Qingyun Yu
- Zhongxing Peng
- Yeqing Huang
- Shengpeng Diao
- Jing Cheng
- Wentao Wang
- Mingfan Hong
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Affiliations: Department of Neurology, Jinan University, Guangzhou, Guangdong, 510632, P.R. China, Department of Neurology, School of Clinical Medicine, First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China, Department of Neurosurgery, School of Clinical Medicine, First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China
Published online on: April 20, 2017 https://doi.org/10.3892/ol.2017.6055
Pages: 4701-4707
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA‑200b (miR‑200b) is a tumor suppressor in multiple tumor types, including gastric cancer, breast cancer, ovarian cancer and glioma. The biological significance of a known normal and cancer stem cell marker, CD133, remains elusive. The aim of the present study was to identify the function and mechinism of miR‑200b in suppressing CD133+ glioma cells. CD133+ glioma cells were sorted by flow cytometry. The expression of miR-200b, Ki67, GAP43, GFAP and CD133 were tested by reverse transcription-quantitative polymerase chain reaction. The binding of miR-200b to prominin 1 (PROM1) was certificated by luciferase reporter assay. Cell proliferation was analyzed by bromodeoxyuridine staining. The protein level of CD133, p-AKT, AKT and Notch1 was detected by western blot analysis. Analysis of glioma samples revealed that CD133 expression is negatively associated with miR‑200b. PROM1, which is the gene that codes CD133, was certified to be a target of miR‑200b. miR‑200b expression inhibited the stemness properties and division of the CD133+ glioma cells. Our results identified a miR‑200b/CD133/PI3K/Akt signaling axis, exploring the fundamental role of miR‑200b and CD133 in glioma stem cell behavior.

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