Characterization of γδ T cells in patients with non-small cell lung cancer

  • Authors:
    • Yi Bao
    • Li Guo
    • Juanfen Mo
  • View Affiliations

  • Published online on: May 17, 2017     https://doi.org/10.3892/ol.2017.6191
  • Pages: 1133-1140
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Abstract

Systemic immune defects that are associated with disease progression exist in a variety of malignancies. γδ T cells are innate‑like lymphocytes that do not require self‑major histocompatibility complex‑restricted priming. Ex vivo‑expanded circulating γδ T cells exhibit promising antitumor activity and are a potential candidate for the treatment of various malignancies, including non‑small cell lung cancer (NSCLC). In the present study, flow cytometry was used as a method to study the phenotypes and characteristics of γδ T cells. A lower frequency of circulating γδ T cells was observed in NSCLC patients than in healthy controls. In advanced NSCLC patients, γδ T cells were also detected in the pleural effusion, but the frequency of γδ T cells here was significantly lower than in the peripheral blood. Vδ1+and Vδ1‑Vδ2‑ T cells represented the most enriched subsets in the pleural effusion. Moreover, the present study demonstrated that Vδ1+ T cells are a type of γδ T cells characterized by a cluster of differentiation (CD)3dim T‑cell receptor (TCR)γδbright phenotype, whereas Vδ2+ T cells represent a CD3brightTCRγδdim phenotype, according to the fluorescence intensity of CD3 and γδTCR using flow cytometry. Finally, the present study reported a decrease in the expression of CD27 and CD28 molecules on the surface of circulating γδ T cells in NSCLC. The present data suggest the existence of a dysregulated repertoire of γδ T cells in NSCLC, which exhibit impaired activation and a reformed cytokine‑releasing profile. Although the ex vivo expansion of γδ T cells may be a prospective therapeutic strategy in NSCLC patients, it remains necessary to clarify which subsets (Vδ1 or Vδ2) should be expanded and the sources from which γδ T cells should be generated.
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July-2017
Volume 14 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Bao Y, Guo L and Mo J: Characterization of γδ T cells in patients with non-small cell lung cancer. Oncol Lett 14: 1133-1140, 2017.
APA
Bao, Y., Guo, L., & Mo, J. (2017). Characterization of γδ T cells in patients with non-small cell lung cancer. Oncology Letters, 14, 1133-1140. https://doi.org/10.3892/ol.2017.6191
MLA
Bao, Y., Guo, L., Mo, J."Characterization of γδ T cells in patients with non-small cell lung cancer". Oncology Letters 14.1 (2017): 1133-1140.
Chicago
Bao, Y., Guo, L., Mo, J."Characterization of γδ T cells in patients with non-small cell lung cancer". Oncology Letters 14, no. 1 (2017): 1133-1140. https://doi.org/10.3892/ol.2017.6191