MicroRNA‑193a‑3p inhibits cell proliferation in prostate cancer by targeting cyclin D1

Liu,Yunfu; Xu,Xin; Xu,Xianglai; Li,Shiqi; Liang,Zhen; Hu,Zhenghui; Wu,Jian; Zhu,Yi; Jin,Xiaodong; Wang,Xiao; Lin,Yiwei; Chen,Hong; Mao,Yeqing; Luo,Jindan; Zheng,Xiangyi; Xie,Liping

doi:10.3892/ol.2017.6865

MicroRNA‑193a‑3p inhibits cell proliferation in prostate cancer by targeting cyclin D1

Authors:
- Yunfu Liu
- Xin Xu
- Xianglai Xu
- Shiqi Li
- Zhen Liang
- Zhenghui Hu
- Jian Wu
- Yi Zhu
- Xiaodong Jin
- Xiao Wang
- Yiwei Lin
- Hong Chen
- Yeqing Mao
- Jindan Luo
- Xiangyi Zheng
- Liping Xie
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Affiliations: Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
Published online on: September 1, 2017 https://doi.org/10.3892/ol.2017.6865
Pages: 5121-5128
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs) are small non‑coding RNAs that affect various biological processes by altering the expression of a target gene. An miRNA microarray analysis has previously revealed a significant decrease in miR‑193a‑3p levels in prostate cancer tissues compared with that in their benign prostate hyperplasia counterparts. However, the role of miR‑193a‑3p has yet to be elucidated. In the present study, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to evaluate the expression levels of miR‑193a‑3p in two human prostate cancer cell lines. Forced overexpression of miR‑193a‑3p was established by transfecting mimics into DU‑145 and PC3 cell lines. Cell proliferation and the cell cycle were assessed using a cell viability assay, flow cytometry and a colony formation assay. In addition, the target gene of miR‑193a‑3p was determined by a luciferase assay, RT‑qPCR and western blot analysis. The regulation of the cell cycle by miR‑193a‑3p was also evaluated by western blotting. The results demonstrated that miR‑193a‑3p expression levels were lower in prostate cancer cell lines as compared with the RWPE normal prostate epithelium cell line. Subsequent gain‑of‑function studies revealed that stable miR‑193a‑3p transfection inhibited cell viability, proliferation and colony formation, and induced G1 phase arrest in prostate cancer cells. A luciferase assay and western blot analysis identified cyclin D1 (CCND1) as a direct target gene of miR‑193a‑3p. In addition, the forced expression of CCND1 was able to counter the inhibitory effects of miR‑193a‑3p transfection in the prostate cancer cells. In summary, the results suggest that miR‑193a‑3p may inhibit the viability, proliferation and survival of prostate cancer cells by regulating the expression profile of CCND1, and that miR‑193a‑3p may be a novel therapeutic biomarker for prostate cancer.

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