Long non-coding RNA HOTAIR up‑regulates chemokine (C‑C motif) ligand 2 and promotes proliferation of macrophages and myeloid‑derived suppressor cells in hepatocellular carcinoma cell lines

Fujisaka,Yasuyuki; Iwata,Tomoaki; Tamai,Keiichi; Nakamura,Mao; Mochizuki,Mai; Shibuya,Rie; Yamaguchi,Kazunori; Shimosegawa,Tooru; Satoh,Kennichi

doi:10.3892/ol.2017.7322

Long non-coding RNA HOTAIR up‑regulates chemokine (C‑C motif) ligand 2 and promotes proliferation of macrophages and myeloid‑derived suppressor cells in hepatocellular carcinoma cell lines

Authors:
- Yasuyuki Fujisaka
- Tomoaki Iwata
- Keiichi Tamai
- Mao Nakamura
- Mai Mochizuki
- Rie Shibuya
- Kazunori Yamaguchi
- Tooru Shimosegawa
- Kennichi Satoh
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Affiliations: Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori, Miyagi 981‑1293, Japan, Division of Molecular and Cellular Oncology, Miyagi Cancer Center Research Institute, Natori, Miyagi 981‑1293, Japan, Department of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980‑8575, Japan
Published online on: November 1, 2017 https://doi.org/10.3892/ol.2017.7322
Pages: 509-514

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Abstract

Accumulating evidence demonstrated that Hox antisense intergenic RNA (HOTAIR) serves essential roles in the development and metastasis of several types of cancer. In hepatocellular carcinoma (HCC), high expression of HOTAIR is associated with poor prognosis, and HOTAIR regulates cell migration and proliferation. However, the downstream molecular targets of HOTAIR depend on the cancer cell types, and little is known about the precise molecular mechanisms of HOTAIR involved in cancer development. The present study investigated the role of HOTAIR in HCC cell lines. Notably, the overexpression of HOTAIR in HCC cell lines did not affect cell migration and proliferation capability. In the microarray analysis, C‑C motif chemokine ligand (CCL)2 was identified to be differentially expressed in HOTAIR‑overexpressing cells, and it was confirmed that HOTAIR promotes the secretion of CCL2. Furthermore, it was revealed that the proportion of macrophages and myeloid‑derived suppressor cells (MDSCs) were increased when peripheral blood mononuclear cells were co‑cultured with HOTAIR‑overexpressing cells. Collectively, these data suggest that HOTAIR regulates CCL2 expression, which may be involved in the recruitment of macrophages and MDSCs to the tumor microenvironment.

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