miR-494 inhibits cervical cancer cell proliferation through upregulation of SOCS6 expression
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- Published online on: December 19, 2017 https://doi.org/10.3892/ol.2017.7651
- Pages: 3075-3080
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Abstract
It is unclear how microRNA (miR)‑494 inhibits the proliferation of cervical cancer cells by altering the expression of SOCS6. Therefore, the present study aimed to investigate the molecular mechanism underlying miR‑494 regulation of suppressor of cytokine signaling 6 (SOCS6) in human cervical cancer samples and the human cervical cancer HeLa cell line. The expression of miR‑494 was determined using reverse transcription‑quantitative polymerase chain reaction. In addition, TargetScan was used to predict miR‑494 target genes and the luciferase reporter assay was used to determine whether SOCS6 was a direct target of miR‑494. The results of the present study demonstrated that compared with the cervical intraepithelial neoplasia and normal cervical tissues, the miR‑494 expression level in cervical cancer samples was significantly decreased (P<0.01). In addition, compared with normal cervical tissue, miR‑494 expression level was significantly decreased in cervical intraepithelial lesions (P<0.05). Furthermore, the expression of miR‑494 was associated with patients with or without lymph node metastasis, clinical stage and depth of stromal invasion (P<0.01); however, miR‑494 expression was not identified to be associated with age, tumor size and menopausal status (P>0.05). Transfection of a miR‑494 mimic significantly increased the expression level of miR‑494 in HeLa cells (P<0.01), and anti‑miR‑494 transfection decreased the expression of miR‑494 (P<0.01). An MTT proliferation assay and Boyden chamber invasion ability assay revealed that miR‑494 mimic transfection significantly inhibited the proliferation, and invasion ability of HeLa cells (P<0.01), whereas anti‑miR‑494 transfection significantly increased the proliferation and invasion ability (P<0.05). SOCS6 was predicted, using bioinformatics, to be the target gene of miR‑494 and this was validated using a luciferase reporter assay. Western blot analysis revealed that transfection of miR‑494 significantly increased the expression of SOCS6 in HeLa cells, and transfection of anti‑miR‑494 significantly decreased the expression of SOCS6. Therefore, the results of the present study demonstrated that miR‑494 expression in cervical cancer was significantly decreased. Exhibiting a decreased expression level of miR‑494 may result in enhanced proliferative and invasive abilities of HeLa cell, thus contributing to the occurrence, and development of cervical cancer.
