MED28 increases the colony‑forming ability of breast cancer cells by stabilizing the ZNF224 protein upon DNA damage

  • Authors:
    • Jin Gu Cho
    • Key‑Hwan Lim
    • Sang Gyu Park
  • View Affiliations

  • Published online on: December 29, 2017     https://doi.org/10.3892/ol.2017.7718
  • Pages: 3147-3154
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Abstract

The regulation of gene expression by transcription factors serves a critical function in cell proliferation. Zinc‑finger protein 224 (ZNF224), a Krüppel‑associated‑box‑containing zinc finger protein, is known to serve a crucial function in integrating the transcriptional co‑factors that activate transcriptional regulation pathways in the cell. A previous study demonstrated that ZNF224 enhances cell proliferation by downregulating the expression of p21 and p53. The present study identified mediator complex subunit 28 (MED28) as a potential binding partner for ZNF224; this was confirmed by co‑immunoprecipitation and a surface plasmon resonance assay. Additionally, the KRAB domain at the N‑terminal of ZNF224 interacts with the MED domain of MED28. Bimolecular fluorescence complementation analysis revealed that ZNF224 associates with MED28 in the nucleus. In addition, ZNF224 was rapidly degraded upon treatment with the DNA‑damaging agent camptothecin (CPT). Transient overexpression of MED28 inhibited the CPT‑mediated degradation of ZNF224, resulting in increased colony formation by MCF‑7 cells. The molecular mechanisms that underlie the biological outcomes of MED28 expression have not yet been fully elucidated. The present study provides molecular evidence for the function of ZNF224 and MED28 in the DNA‑damage response.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cho J, Lim KH and Park S: MED28 increases the colony‑forming ability of breast cancer cells by stabilizing the ZNF224 protein upon DNA damage. Oncol Lett 15: 3147-3154, 2018.
APA
Cho, J., Lim, K., & Park, S. (2018). MED28 increases the colony‑forming ability of breast cancer cells by stabilizing the ZNF224 protein upon DNA damage. Oncology Letters, 15, 3147-3154. https://doi.org/10.3892/ol.2017.7718
MLA
Cho, J., Lim, K., Park, S."MED28 increases the colony‑forming ability of breast cancer cells by stabilizing the ZNF224 protein upon DNA damage". Oncology Letters 15.3 (2018): 3147-3154.
Chicago
Cho, J., Lim, K., Park, S."MED28 increases the colony‑forming ability of breast cancer cells by stabilizing the ZNF224 protein upon DNA damage". Oncology Letters 15, no. 3 (2018): 3147-3154. https://doi.org/10.3892/ol.2017.7718