Therapeutic targeting of noncoding RNAs in hepatocellular carcinoma: Recent progress and future prospects (Review)
- Authors:
- Published online on: January 9, 2018 https://doi.org/10.3892/ol.2018.7758
- Pages: 3395-3402
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Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Advanced RNA sequencing technology has facilitated the identification of substantial amounts of noncoding RNAs (ncRNAs). It was reported that up to 98% of the human genome encodes ncRNAs, which were previously considered to be transcriptional noise (1–3). Although they have no protein-coding capacity, these RNAs directly function as structural, catalytic and regulatory RNAs. Previous studies have demonstrated their ability to serve a plethora of roles in a variety of molecular functions including the regulation of transcription, splicing, and translation, in addition to the maturation of other ncRNAs which have been extensively reviewed previously (4–8). Small noncoding RNAs (sncRNAs), with a size of <200 nucleotides, were first identified by exogenous RNA interference (RNAi) in plants and nematodes, and were later found to exist endogenously (9). Currently, sncRNAs are classified as small interfering RNAs (siRNAs), microRNAs (miRNAs) and piwi-interacting RNA (piRNAs), which mainly function as gene-regulators in animals and plants by pairing to targeted genes and directing their post-transcriptional repression (10). Approximately 2,000 ‘high confidence’ miRNAs have been reported in the human genome (miRBase 21, released July 3rd, 2014, http://www.mirbase.org/). Beyond the sncRNAs, the pervasive transcription of long noncoding RNAs (lncRNAs), with a size of >200 nucleotides, has also been discovered. lncRNAs include long intergenic noncoding RNAs, natural antisense transcripts, transcribed ultra-conserved regions and noncoding pseudogenes. Although the amount of lncRNAs is far greater than that of coding RNAs in mammals (11), the functions of the majority of lncRNAs remain unknown (12). Comprehensive analysis of published studies revealed three main modes of lncRNA-mediated regulation: i) Guiding the protein to a specific genomic region for gene regulation; ii) acting as the scaffold for the formation of functioning protein complex; and iii) competing with other factors for binding to the protein (13). An increasing amount of evidence demonstrates that ncRNAs play various biological roles in a wide range of cellular activities, including cellular function, pathways, motility, structure and stem cell biology (14,15). For example, it was reported that the lncRNA HOTTIP promoted cell proliferation and migration via activating the Wnt/β-catenin pathway (16). ncRNAs also serve regulatory functions in diverse pathological processes, including a number of malignancies (17–19). Due to the lack of studies on the majority of types of ncRNAs in HCC, the current review will focus on piRNA, miRNA and lncRNA, which have been extensively investigated in the pathogenesis of HCC.
sncRNAs in HCC
miRNAs in HCC
miRNAs are small molecules of ~22 nucleotides in length, that are able to regulate gene expression by inhibiting target mRNA translation or by inducing mRNA degradation through partial or complementary binding to the 3′-untranslated regions of target genes (10,20). miRNAs are involved in numerous biological processes, including embryonic development, differentiation, proliferation, apoptosis, invasion and autophagy (21–24). Aberrant expression of miRNAs has been implicated in numerous diseases, including cancer (25). The roles of miRNAs in cancer can be oncogenic or tumor-suppressive, as the regulatory targets of the miRNAs determine their effect on carcinogenesis.
Extensive data from previous studies describe the multiple roles of miRNAs in HCC. Decreased or silenced expression of tumor-suppressive miRNAs have the potential to contribute to the pathogenesis of HCC through increasing the expression of their oncogenic targets. Previous studies have demonstrated that downregulation of tumor-suppressive miRNAs, such as miR-122a, miR-34a, miR-199a and miR-200, can lead to the activation of expression of their oncogenic targets in HCC (26–29). Meanwhile, the effect of increased oncogenic miRNAs, such as miR-21, miR-182 and miR-221, may also lead to the suppression of anti-oncogenes in HCC (30–32).
Accumulating evidence based on dysregulated miRNAs in HCC demonstrates the involvement of miRNAs in different stages of tumor progression (33–35). Previous studies also demonstrated that miRNA profiling enables the classification of various types of tumor including HCC (36), and that these profiles can act as signatures associated with diagnosis, prognosis and response to drug treatment in cancer (36,37). Recently, circulating miRNAs were demonstrated to reflect different pathophysiological conditions of HCC, indicating that miRNAs may constitute promising and clinically useful biomarkers for this cancer type (38). Although vast quantities of miRNAs and their direct targets have been identified in HCC, the molecular mechanisms of interplay between miRNAs and their upstream regulators are still lacking. Previous studies identified that the expression levels of miRNAs are subject to transcriptional and post-transcriptional regulation, with miR-200, miR-20a, miR-106b and miR-941 were reported to be epigenetically regulated in HCC (39–41). Specific transcriptional factors, such as p53, heat shock transcription factor 1 and forkhead box D3, have been hypothesized to bind directly to miRNA promoters to regulate miRNA expression in liver cancer (42,43).
piRNAs in HCC
piRNAs are small molecules of 26–31 nucleotides in length and are the largest class of known sncRNA molecules expressed in animal cells (44,45). piRNAs interact with the PIWI subfamily of argonaute proteins to form functional RNA-protein complexes (46,47), which developed as a conserved defense mechanism to protect the genetic information of animal germ cells from the detrimental effects of molecular parasites, such as transposons (48). piRNAs have been identified as regulators for the development and maintenance of DNA integrity by transcriptional or post-transcriptional gene silencing. Typically, such post-transcriptional gene silencing is sequence-specific and is subject to catalytically active enzymes (49–52). piRNAs can also direct gene regulation through chromatin modifications, including repressive histone marks and DNA methylation (53–55). It was also reported that piRNAs are involved in the epigenetic silencing of transposable elements (56).
PIWI proteins serve key roles in the biogenesis and functions of piRNAs by directly binding to piRNAs (57). Previous reports elucidated the function of the piRNA/PIWI axis in various human cancer types (58,59). PIWI proteins are reported to be aberrantly and ectopically expressed in many cancer types, including HCC (56,60–62). In these studies, PIWI proteins were found to exert multiple functional roles in cancer, such as promoting cell proliferation, inhibiting cell apoptosis, facilitating cell migration and invasion, regulating genomic integrity and acting as potential biomarkers for cancer diagnosis and prognosis. Regarding the specific role of PIWI proteins in HCC, it was reported that the expression of PIWI proteins, including PIWIL1, was increased in HCC cells, with depletion of this protein culminating in decreased invasion and metastasis (62,63). Meanwhile, the presence of PIWI protein was also an independent risk factor affecting the overall survival and recurrence-free survival of HCC patients (62).
In addition to the crucial role of PIWI proteins in tumorigenesis, piRNAs also serve a vital role in this process. Certain piRNAs, such as tRNA-derived RNA fragments, may be associated with PIWI proteins in cancer cells (64). At present, numerous piRNAs have been identified to be associated with cancer pathology, including human piRABC, piR-Hep1, piR-651, piR-823, piR-932, piR-4987, piR-20365, piR-20485 and piR-20582. piRNAs are dysregulated in different cancer types, with functional investigations of these piRNAs demonstrating that these RNAs inhibited cell proliferation and colony formation, regulated cell cycle, promoted cell apoptosis, correlated well with clinical stages of patients, and decreased proangiogenic activity in cancer (60,65–73). However, only a few studies have reported the role of piRNAs in HCC. piR-Hep1 expression was upregulated in ~50% of hepatic tumors as compared with the corresponding adjacent tissues. Further studies identified that piR-Hep1 inhibited cell viability, motility and invasiveness in HCC (70), which provided novel insights into this cancer type. However further study is necessary to determine the role of piRNAs in HCC.
Targeting sncRNAs in HCC therapy
sncRNAs antagonism for HCC therapy
In conditions where miRNAs or piRNAs act as oncogenes in cancer, strategies involving the antagonism of miRNA or piRNA activity are desirable. Two broad categories of molecules can be applied to block sncRNAs: i) Oligonucleotide miRNA/piRNA inhibitors; and ii) RNA sponges containing the RNA oligonucleotides complimentary to the target miRNAs or piRNAs.
Nucleic acid inhibitors can inhibit endogenous miRNAs or piRNAs that exhibit tumor-promoting functions in cancer. An miR-122 inhibitor has been tested in phase 2a clinical trials for treating patients with hepatitis C virus (HCV) infection. This provides a promising prospect for using antagonists of sncRNAs in liver disease therapy (74). Antagonists are typically chemically modified to bind to the target sncRNAs with high affinity. The functions of sncRNAs can be impeded or silenced by these antagonists due to antagonist sncRNA duplexes being unable to incorporate into the RNA-induced silencing complex as a result of high RNA duplex stability. Currently, the majority of advanced anti-miRNA therapies are in the preclinical stage; however, further studies are required to confirm their efficacy in cancer therapy. piRNA antagonists have also been reported in different cancer types (69). For example it was reported that piR-651 antagonists could induce cell cycle arrest in gastric cancer cells (69). Nevertheless, at present there are no studies reporting the use of piRNA antagonists in the treatment of HCC.
In addition to the nucleic acid inhibitors of miRNAs, researchers recently discovered that certain small molecules can also inhibit the expression of specific miRNAs in different cancer types; for example, inhibitors of miR-122 can inhibit HCV replication in liver cells (75). Additionally, a small molecule inhibitor of miR-525 was screened from a compound library, and it was revealed that this could inhibit the production of mature miR-525, upregulate the downstream protein ZNF395, and inhibit invasion of HCC cells (76). A powerful online database, The Connectivity Map, which uses gene-expression signatures to connect small molecules, genes, and disease (77,78), has provided a novel way to identify potential modulators of sncRNAs for cancer therapy. The gene-expression profiles following the gain or loss of sncRNAs in cancer cells may be used to compare with the gene-expression profiles obtained from cells following treatment with different compounds.
sncRNA replacement for HCC therapy
Conversely, the loss of sncRNAs can be reversed via utilizing RNA mimics to correct RNA deficiency. To improve their endogenous protective mechanisms, RNA mimics have their own strands resembling the sequences of the respective targets. As miRNA mimics may exhibit similar functions to tumor-suppressive small ncRNAs in cancer cells, they are potential anti-cancer therapeutics. It is reported that the delivery of miR-26a mimics can inhibit tumor progression in a murine HCC model (79). Fang et al (80) also reported that miR-188-5p mimics suppressed HCC cell proliferation and metastasis in vitro and in vivo. Another previous study identified that miR-95a mimics inhibit HCC growth (81). In addition, a piRNA mimic of piR-015520 was demonstrated to repress the expression of its target gene, melatonin receptor 1A (82). As such, the therapeutic effects involve the reactivation of pathways unfavorable to cancer cells.
In addition to the nucleic acid approach to sncRNA replacement (i.e., sncRNA mimics), certain small molecules have also been identified to activate the expression of sncRNAs in cancer. Various studies have screened small molecules that induce sncRNA expression in liver cancer. In HCC, Young et al (75) discovered that a small molecule may upregulate miR-122 in HCC cells, and subsequently upregulate caspase expression, culminating in apoptosis and reduced cell viability. Additionally, by screening from a nature products library, our previous study identified a small molecule named Rubone that may activate tumor-suppressive miR-34a expression in HCC cells (27). It was identified that miR-34a activation could increase miR-34a promoter activity via increasing p53 occupancy on the miR-34a promoter in wild type and mutant p53. Furthermore, our study demonstrated that a miR-34a activator significantly inhibited HCC cell growth and tumor growth by silencing the downstream oncogenic targets of this miRNA (27). However, small molecules that can modulate piRNA expression are still lacking. miRNA-based anti-HCC therapy has great potential, as reports have demonstrated that few adverse reactions would be caused in normal tissues when administered with an miRNA-based agent. The Connectivity Map tool may also be used to screen the activators of sncRNAs; for example, when gene expression profiles exhibit a high similarity following compound treatment or sncRNA overexpression, this indicates that the compound may be an activator of the corresponding sncRNA.
lncRNAs in HCC
Functional role of lncRNAs in HCC
The primary role of lncRNAs is to act as an adaptor that can mediate interactions between DNA, proteins and other RNAs (83). Previous mechanistic investigations revealed that lncRNAs exert their functional roles predominantly in two ways. First, they can bind directly to DNA or other RNA molecules (84). Second, lncRNAs may form secondary structures that function as binding sites for proteins or small molecules (85,86). The aforementioned properties of lncRNAs may enable a much broader range of functions than with sncRNAs. Additionally, the increased number of binding sites that lncRNAs contain may allow more functional interactions when compared with sncRNAs. Studies have demonstrated that lncRNAs serve critical roles in cellular processes, with specific lncRNAs possessing the ability to modulate cancer epigenomes and contribute to different pathological conditions, such as proliferation, apoptosis, metastasis, migration and epithelial-to-mesenchymal transition (EMT) (87–90). Consequently, comprehension of the molecular mechanisms of lncRNAs in tumor development and progression may provide a novel avenue in cancer therapy.
The roles of lncRNAs in HCC have been reported in recent publications (91–94). In these studies, the upregulated lncRNAs [including hepatocellular carcinoma upregulated long non-coding RNA (HULC); H19; PCBP2 overlapping transcript 1/transcribed ultra conserved region 338; metastasis-associated lung adenocarcinoma transcript 1 (MALAT1); HOX transcript antisense RNA (HOTAIR); HOXA distal transcript antisense RNA (HOTTIP); hepatocellular carcinoma upregulated EZH2-associated long non-coding RNA (HEIH); ribosomal oxygenase 2/mineral dust-induced gene; plasmacytoma variant translocation 1 (PVT1); long intergenic non-protein coding RNA 974 (LINC00974); ubiquitin-fold modifier conjugating enzyme 1 (UFC1); PCNA antisense RNA 1; urothelial cancer-associated 1; colon cancer-associated transcript 1 (CCAT1); neutral amino acid transporter B (ATB); and upregulated in hepatocellular carcinoma (URHC)], in addition to the downregulated lncRNAs [including maternally expressed 3 (MEG3/GTL2); phosphatase and tensin homolog pseudogene 1 (PTENP1); long intergenic non-protein coding RNA 1018 (LINC01018/SRHC); and methallothionein 1D pseudogene (MT1DP)], are summarized in detail. In these studies, the lncRNAs in HCC were also divided into two groups: i) lncRNAs associated with tumor growth and proliferation (such as PTENP1, MEG3, CCAT1, ZNRD1 antisense RNA 1, UFC1, lncRNA-hPVT1 and HULC); and ii) lncRNAs associated with metastasis and prognosis (such as H19, MALAT1, HOTAIR, HOTTIP, HEIH, ATB and lncRNA-p21). Tang et al (95) discovered novel lncRNAs associated with HCC that have also been identified to have multiple functions; for example LINC00974 can activate the transforming growth factor-β and Notch signaling pathways, which promote the invasion and proliferation of HCC. High levels of URHC can inhibit tumor growth via activation of tumor-suppressive gene expression in HCC (96,97). SRHC inhibited cell proliferation and promoted cell differentiation in HCC (98). MT1DP has demonstrated an ability to inhibit the transformative phenotype of liver cancer cells and cell proliferation (99). However, more preclinical models of HCC are required to provide more support for the clinical applications of lncRNAs.
Targeting lncRNAs in HCC therapy
Owing to the large amount of lncRNAs that have been implicated in HCC, these RNAs represent rational candidates for potential use in HCC therapy. According to previously published literature, in HCC, a greater number of upregulated lncRNAs than downregulated lncRNAs have been identified (94,100). Therapeutic strategies that reduce the endogenous transcript levels of lncRNAs may have more favorable results for HCC therapy. Currently, RNAi-based techniques are extensively used to inhibit lncRNAs in HCC cells. Du et al (101) reported that knockdown of HULC and MALAT1 by RNAi-based technique can inhibit HCC cell proliferation. It was also reported that using antisense oligonucleotides, which are short, single-stranded DNAs or RNAs designed with antisense sequences of their target RNAs, could silence lncRNA function through degradation of lncRNA transcripts (102,103). For example, antisense oligonucleotides directed against insulin-like growth factor-II mRNAs could delay the progress of HCC (104). Hammerhead ribozyme (HamRz) is another method to silence lncRNAs in liver cancer via destabilizing the phosphodiester backbone of its targets (105). Previous research has demonstrated that targeting lncRNAs with HamRz, has anti-cancer potential in different cancer types, including colorectal and lung (106). However, to date, there has been no research on the role of HamRz in HCC, which needs further study.
On the other hand, information on the secondary structure of lncRNAs can be utilized to screen potential modulators of this class of RNAs. The specificity and affinity of aptamers to the secondary structure of the target RNA are much higher than those of the complementary oligonucleotides (107). Although aptamers were reported to modulate lncRNAs through secondary structure interaction (108,109), presently, there have been no studies examining this strategy in cancer therapy, which may be a novel direction for investigating modulators of lncRNAs in HCC.
Comparable to the strategy for screening the modulators of sncRNAs, small molecules also have the potential to modulate lncRNAs. Three compounds were identified to selectively modulate lncRNAs expression and subsequently repair various disorders in mice (110–112). Previous research demonstrated the feasibility of using a high-throughput screening method to identify modulators of lncRNAs (113). This could facilitate the development of a method utilizing small molecules to target lncRNAs that are dysregulated in HCC as a potential cancer therapy. The Connectivity Map tool is another strategy to elucidate modulators of lncRNAs in future studies.
Conclusions
HCC continues to be one of the most common malignancies and is a leading cause of cancer-associated fatalities due to its high mortality rate and the limited therapeutic options. Presently, sorafenib is the sole drug that has been approved for first-line treatment of HCC (114–116). Owing to recent failures of clinical trials undertaken to investigate novel drugs for HCC, the development of more effective drugs is urgently required.
ncRNAs exert their genomic regulation via transcriptional, post-transcriptional, and epigenetic modification, and the pathways that ncRNAs are involved in play crucial roles in HCC progression (Fig. 1). Various preclinical studies suggest that modulation of ncRNAs can elicit significant anti-cancer effects. In previous studies, a large number of ncRNAs, including miRNAs and lncRNAs, have been identified and several ncRNAs have been investigated in HCC (117). However, no ncRNAs have yet entered into clinical practice, with the exception of several that have been investigated in phase I and II clinical trials. HCC therapeutic treatments based on the targeting ncRNAs require further assessment. In addition, efficient delivery of agents to the targeted ncRNAs in HCC therapy remains a crucial issue. Nucleic acid-based drugs are well-established agents in targeting ncRNAs; however, the delivery of this drug type remains challenging including low biological stability and the poor tissue penetrance and cellular uptake, making it hard to pursue (118,119). Meanwhile, nucleic acid-based drugs are usually poor in terms of accessibility into cancer cells. Small molecules with low molecular weight typically possess superior biological effects; numerous commonly used potent anti-cancer drugs, including erlotinib, gefitinib and sorafenib, are small molecules (120). Small molecules also show significant advantages for anti-cancer drug development for the following reasons: i) A wide range of small molecules can be synthesized within a short time; ii) compounds can be easily screened for interactions with a target; and iii) they are orally bioavailable and cost-effective (121). However, the safety of small molecule-based drugs must be established and verified. Therefore, nucleic acid and small molecule-based agents require further investigation in preclinical studies, and show promising potential as a drug of choice for HCC treatment in clinical practice.
Acknowledgements
This study was supported by grants from the National Natural Science Foundation of China (grant nos. 81503093, 81473269, 81602166 and 81672444).
References
|
International Human Genome Sequencing Consortium, . Finishing the euchromatic sequence of the human genome. Nature. 431:931–945. 2004. View Article : Google Scholar : PubMed/NCBI | |
|
Yamada K, Lim J, Dale JM, Chen H, Shinn P, Palm CJ, Southwick AM, Wu HC, Kim C, Nguyen M, et al: Empirical analysis of transcriptional activity in the Arabidopsis genome. Science. 302:842–846. 2003. View Article : Google Scholar : PubMed/NCBI | |
|
Pennisi E: Shining a light on the genome's ‘dark matter’. Science. 330:16142010. View Article : Google Scholar : PubMed/NCBI | |
|
He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI | |
|
Miska EA: How microRNAs control cell division, differentiation and death. Curr Opin Genet Devel. 15:563–568. 2005. View Article : Google Scholar | |
|
Nagano T and Fraser P: No-nonsense functions for long noncoding RNAs. Cell. 145:178–181. 2011. View Article : Google Scholar : PubMed/NCBI | |
|
Rinn JL and Chang HY: Genome regulation by long noncoding RNAs. Ann Rev Biochem. 81:145–166. 2012. View Article : Google Scholar : PubMed/NCBI | |
|
Khanduja JS, Calvo IA, Joh RI, Hill IT and Motamedi M: Nuclear noncoding RNAs and genome stability. Mol Cell. 63:7–20. 2016. View Article : Google Scholar : PubMed/NCBI | |
|
Hannon GJ, Rivas FV, Murchison EP and Steitz JA: The expanding universe of noncoding RNAs. Cold Spring Harb Symp Quant Biol. 71:551–564. 2006. View Article : Google Scholar : PubMed/NCBI | |
|
Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI | |
|
Djebali S, Davis CA, Merkel A, Dobin A, Lassmann T, Mortazavi A, Tanzer A, Lagarde J, Lin W, Schlesinger F, et al: Landscape of transcription in human cells. Nature. 489:101–108. 2012. View Article : Google Scholar : PubMed/NCBI | |
|
Takahashi K, Yan I, Haga H and Patel T: Long noncoding RNA in liver diseases. Hepatology. 60:744–753. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Guttman M, Donaghey J, Carey BW, Garber M, Grenier JK, Munson G, Young G, Lucas AB, Ach R, Bruhn L, et al: lincRNAs act in the circuitry controlling pluripotency and differentiation. Nature. 477:295–300. 2011. View Article : Google Scholar : PubMed/NCBI | |
|
Tao H, Yang JJ and Shi KH: Non-coding RNAs as direct and indirect modulators of epigenetic mechanism regulation of cardiac fibrosis. Expert Opin Ther Targets. 19:707–716. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Fu XD: Non-coding RNA: A new frontier in regulatory biology. Nat Sci Rev. 1:190–204. 2014. View Article : Google Scholar | |
|
Liao B, Chen R, Lin F, Mai A, Chen J, Li H, Xu Z and Dong S: Long noncoding RNA HOTTIP promotes endothelial cell proliferation and migration via activation of the Wnt/beta-catenin pathway. J Cell Biochem. 2017. | |
|
Peng JF, Zhuang YY, Huang FT and Zhang SN: Noncoding RNAs and pancreatic cancer. World J Gastroenterol. 22:801–814. 2016. View Article : Google Scholar : PubMed/NCBI | |
|
Zhang M and Du X: Noncoding RNAs in gastric cancer: Research progress and prospects. World J Gastroenterol. 22:6610–6618. 2016. View Article : Google Scholar : PubMed/NCBI | |
|
Adams BD, Parsons C, Walker L, Zhang WC and Slack FJ: Targeting noncoding RNAs in disease. J Clin Invest. 127:761–771. 2017. View Article : Google Scholar : PubMed/NCBI | |
|
Shukla GC, Singh J and Barik S: MicroRNAs: Processing, maturation, target recognition and regulatory functions. Mol Cell Pharmacol. 3:83–92. 2011.PubMed/NCBI | |
|
Marson A, Levine SS, Cole MF, Frampton GM, Brambrink T, Johnstone S, Guenther MG, Johnston WK, Wernig M, Newman J, et al: Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells. Cell. 134:521–533. 2008. View Article : Google Scholar : PubMed/NCBI | |
|
Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI | |
|
Shivdasani RA: MicroRNAs: Regulators of gene expression and cell differentiation. Blood. 108:3646–3653. 2006. View Article : Google Scholar : PubMed/NCBI | |
|
Wu SY, Lan SH and Liu HS: Autophagy and microRNA in hepatitis B virus-related hepatocellular carcinoma. World J Gastroenterol. 22:176–187. 2016. View Article : Google Scholar : PubMed/NCBI | |
|
Zhang L, Yu J, Wong CC, Ling TK, Li ZJ, Chan KM, Ren SX, Shen J, Chan RL, Lee CC, et al: Cathelicidin protects against Helicobacter pylori colonization and the associated gastritis in mice. Gene Ther. 20:751–760. 2013. View Article : Google Scholar : PubMed/NCBI | |
|
Tsai WC, Hsu SD, Hsu CS, Lai TC, Chen SJ, Shen R, Huang Y, Chen HC, Lee CH, Tsai TF, et al: MicroRNA-122 plays a critical role in liver homeostasis and hepatocarcinogenesis. J Clin Invest. 122:2884–2897. 2012. View Article : Google Scholar : PubMed/NCBI | |
|
Xiao Z, Li CH, Chan SL, Xu F, Feng L, Wang Y, Jiang JD, Sung JJ, Cheng CH and Chen Y: A small-molecule modulator of the tumor-suppressor miR34a inhibits the growth of hepatocellular carcinoma. Cancer Res. 74:6236–6247. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Hou J, Lin L, Zhou W, Wang Z, Ding G, Dong Q, Qin L, Wu X, Zheng Y, Yang Y, et al: Identification of miRNomes in human liver and hepatocellular carcinoma reveals miR-199a/b-3p as therapeutic target for hepatocellular carcinoma. Cancer Cell. 19:232–243. 2011. View Article : Google Scholar : PubMed/NCBI | |
|
Zhang L, Yang F, Yuan JH, Yuan SX, Zhou WP, Huo XS, Xu D, Bi HS, Wang F and Sun SH: Epigenetic activation of the MiR-200 family contributes to H19-mediated metastasis suppression in hepatocellular carcinoma. Carcinogenesis. 34:577–586. 2013. View Article : Google Scholar : PubMed/NCBI | |
|
Trebinjac S, Radulović R and Buljina A: Prevalence of gonarthrosis and the duration of rehabilitation. Med Arh. 43:179–182. 1989.(In Croatian). PubMed/NCBI | |
|
Wang C, Ren R, Hu H, Tan C, Han M, Wang X and Zheng Y: MiR-182 is up-regulated and targeting Cebpa in hepatocellular carcinoma. Chin J Cancer Res. 26:17–29. 2014.PubMed/NCBI | |
|
Park JK, Kogure T, Nuovo GJ, Jiang J, He L, Kim JH, Phelps MA, Papenfuss TL, Croce CM, Patel T and Schmittgen TD: miR-221 silencing blocks hepatocellular carcinoma and promotes survival. Cancer Res. 71:7608–7616. 2011. View Article : Google Scholar : PubMed/NCBI | |
|
Murakami Y, Yasuda T, Saigo K, Urashima T, Toyoda H, Okanoue T and Shimotohno K: Comprehensive analysis of microRNA expression patterns in hepatocellular carcinoma and non-tumorous tissues. Oncogene. 25:2537–2545. 2006. View Article : Google Scholar : PubMed/NCBI | |
|
Budhu A, Jia HL, Forgues M, Liu CG, Goldstein D, Lam A, Zanetti KA, Ye QH, Qin LX, Croce CM, et al: Identification of metastasis-related microRNAs in hepatocellular carcinoma. Hepatology. 47:897–907. 2008. View Article : Google Scholar : PubMed/NCBI | |
|
Ladeiro Y, Couchy G, Balabaud C, Bioulac-Sage P, Pelletier L, Rebouissou S and Zucman-Rossi J: MicroRNA profiling in hepatocellular tumors is associated with clinical features and oncogene/tumor suppressor gene mutations. Hepatology. 47:1955–1963. 2008. View Article : Google Scholar : PubMed/NCBI | |
|
Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, Ebert BL, Mak RH, Ferrando AA, et al: MicroRNA expression profiles classify human cancers. Nature. 435:834–838. 2005. View Article : Google Scholar : PubMed/NCBI | |
|
Xu Y, Huang J, Ma L, Shan J, Shen J, Yang Z, Liu L, Luo Y, Yao C and Qian C: MicroRNA-122 confers sorafenib resistance to hepatocellular carcinoma cells by targeting IGF-1R to regulate RAS/RAF/ERK signaling pathways. Cancer Lett. 371:171–181. 2016. View Article : Google Scholar : PubMed/NCBI | |
|
Afonso MB, Rodrigues PM, Simao AL and Castro RE: Circulating microRNAs as potential biomarkers in non-alcoholic fatty liver disease and hepatocellular carcinoma. J Clin Med. 5:E302016. View Article : Google Scholar : PubMed/NCBI | |
|
Ning X, Shi Z, Liu X, Zhang A, Han L, Jiang K, Kang C and Zhang Q: DNMT1 and EZH2 mediated methylation silences the microRNA-200b/a/429 gene and promotes tumor progression. Cancer Lett. 359:198–205. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Yang H, Lan P, Hou Z, Guan Y, Zhang J, Xu W, Tian Z and Zhang C: Histone deacetylase inhibitor SAHA epigenetically regulates miR-17-92 cluster and MCM7 to upregulate MICA expression in hepatoma. Br J Cancer. 112:112–121. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Zhang PP, Wang XL, Zhao W, Qi B, Yang Q, Wan HY, Shuang ZY, Liu M, Li X, Li S and Tang H: DNA methylation-mediated repression of miR-941 enhances lysine (K)-specific demethylase 6B expression in hepatoma cells. J Biol Chem. 289:24724–24735. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Li Y, Xu D, Bao C, Zhang Y, Chen D, Zhao F, Ding J, Liang L, Wang Q, Liu L, et al: MicroRNA-135b, a HSF1 target, promotes tumor invasion and metastasis by regulating RECK and EVI5 in hepatocellular carcinoma. Oncotarget. 6:2421–2433. 2015.PubMed/NCBI | |
|
Liu LL, Lu SX, Li M, Li LZ, Fu J, Hu W, Yang YZ, Luo RZ, Zhang CZ and Yun JP: FoxD3-regulated microRNA-137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting AKT2. Oncotarget. 5:5113–5124. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Kojima T: Kanagawa prefectural institute of public health. Chudoku Kenkyu. 22:266–269. 2009.(In Japanese). PubMed/NCBI | |
|
Pantano L, Jodar M, Bak M, Ballescà JL, Tommerup N, Oliva R and Vavouri T: The small RNA content of human sperm reveals pseudogene-derived piRNAs complementary to protein-coding genes. RNA. 21:1085–1095. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Girard A, Sachidanandam R, Hannon GJ and Carmell MA: A germline-specific class of small RNAs binds mammalian Piwi proteins. Nature. 442:199–202. 2006.PubMed/NCBI | |
|
Grivna ST, Beyret E, Wang Z and Lin H: A novel class of small RNAs in mouse spermatogenic cells. Genes Dev. 20:1709–1714. 2006. View Article : Google Scholar : PubMed/NCBI | |
|
Czech B and Hannon GJ: One loop to rule them all: The Ping-Pong cycle and piRNA-guided silencing. Trends Biochem Sci. 41:324–337. 2016. View Article : Google Scholar : PubMed/NCBI | |
|
Li C, Vagin VV, Lee S, Xu J, Ma S, Xi H, Seitz H, Horwich MD, Syrzycka M, Honda BM, et al: Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. Cell. 137:509–521. 2009. View Article : Google Scholar : PubMed/NCBI | |
|
De Fazio S, Bartonicek N, Di Giacomo M, Abreu-Goodger C, Sankar A, Funaya C, Antony C, Moreira PN, Enright AJ and O'Carroll D: The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements. Nature. 480:259–263. 2011. View Article : Google Scholar : PubMed/NCBI | |
|
Reuter M, Berninger P, Chuma S, Shah H, Hosokawa M, Funaya C, Antony C, Sachidanandam R and Pillai RS: Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing. Nature. 480:264–267. 2011. View Article : Google Scholar : PubMed/NCBI | |
|
Wang W, Yoshikawa M, Han BW, Izumi N, Tomari Y, Weng Z and Zamore PD: The initial uridine of primary piRNAs does not create the tenth adenine that Is the hallmark of secondary piRNAs. Mol Cell. 56:708–716. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Pezic D, Manakov SA, Sachidanandam R and Aravin AA: piRNA pathway targets active LINE1 elements to establish the repressive H3K9me3 mark in germ cells. Genes Dev. 28:1410–1428. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Itou D, Shiromoto Y, Yukiho SY, Ishii C, Nishimura T, Ogonuki N, Ogura A, Hasuwa H, Fujihara Y, Kuramochi-Miyagawa S and Nakano T: Induction of DNA methylation by artificial piRNA production in male germ cells. Current Biol. 25:901–906. 2015. View Article : Google Scholar | |
|
Yan H, Wu QL, Sun CY, Ai LS, Deng J, Zhang L, Chen L, Chu ZB, Tang B, Wang K, et al: piRNA-823 contributes to tumorigenesis by regulating de novo DNA methylation and angiogenesis in multiple myeloma. Leukemia. 29:196–206. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Ng KW, Anderson C, Marshall EA, Minatel BC, Enfield KS, Saprunoff HL, Lam WL and Martinez VD: Piwi-interacting RNAs in cancer: Emerging functions and clinical utility. Mol Cancer. 15:52016. View Article : Google Scholar : PubMed/NCBI | |
|
Juliano C, Wang J and Lin H: Uniting germline and stem cells: The function of Piwi proteins and the piRNA pathway in diverse organisms. Ann Rev Genet. 45:447–469. 2011. View Article : Google Scholar : PubMed/NCBI | |
|
Moyano M and Stefani G: piRNA involvement in genome stability and human cancer. J Hematol Oncol. 8:382015. View Article : Google Scholar : PubMed/NCBI | |
|
Assumpção CB, Calcagno DQ, Araújo TM, Santos SE, Santos ÂK, Riggins GJ, Burbano RR and Assumpção PP: The role of piRNA and its potential clinical implications in cancer. Epigenomics. 7:975–984. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Kwon C, Tak H, Rho M, Chang HR, Kim YH, Kim KT, Balch C, Lee EK and Nam S: Detection of PIWI and piRNAs in the mitochondria of mammalian cancer cells. Biochem Biophys Res Commun. 446:218–223. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Chen C, Liu J and Xu G: Overexpression of PIWI proteins in human stage III epithelial ovarian cancer with lymph node metastasis. Cancer Biomark. 13:315–321. 2013. View Article : Google Scholar : PubMed/NCBI | |
|
Zhao YM, Zhou JM, Wang LR, He HW, Wang XL, Tao ZH, Sun HC, Wu WZ, Fan J, Tang ZY and Wang L: HIWI is associated with prognosis in patients with hepatocellular carcinoma after curative resection. Cancer. 118:2708–2717. 2012. View Article : Google Scholar : PubMed/NCBI | |
|
Xie Y, Yang Y, Ji D, Zhang D, Yao X and Zhang X: Hiwi downregulation, mediated by shRNA, reduces the proliferation and migration of human hepatocellular carcinoma cells. Mol Med Rep. 11:1455–1461. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Keam SP, Young PE, McCorkindale AL, Dang TH, Clancy JL, Humphreys DT, Preiss T, Hutvagner G, Martin DI, Cropley JE and Suter CM: The human Piwi protein Hiwi2 associates with tRNA-derived piRNAs in somatic cells. Nucleic Acids Res. 42:8984–8995. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Hashim A, Rizzo F, Marchese G, Ravo M, Tarallo R, Nassa G, Giurato G, Santamaria G, Cordella A, Cantarella C and Weisz A: RNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer. Oncotarget. 5:9901–9910. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Lu Y, Li C, Zhang K, Sun H, Tao D, Liu Y, Zhang S and Ma Y: Identification of piRNAs in Hela cells by massive parallel sequencing. BMB Rep. 43:635–641. 2010. View Article : Google Scholar : PubMed/NCBI | |
|
Cheng J, Deng H, Xiao B, Zhou H, Zhou F, Shen Z and Guo J: piR-823, a novel non-coding small RNA, demonstrates in vitro and in vivo tumor suppressive activity in human gastric cancer cells. Cancer Lett. 315:12–17. 2012. View Article : Google Scholar : PubMed/NCBI | |
|
Huang G, Hu H, Xue X, Shen S, Gao E, Guo G, Shen X and Zhang X: Altered expression of piRNAs and their relation with clinicopathologic features of breast cancer. Clin Trans Oncol. 15:563–568. 2013. View Article : Google Scholar | |
|
Cheng J, Guo JM, Xiao BX, Miao Y, Jiang Z, Zhou H and Li QN: piRNA, the new non-coding RNA, is aberrantly expressed in human cancer cells. Clin Chim Acta. 412:1621–1625. 2011. View Article : Google Scholar : PubMed/NCBI | |
|
Law PT, Qin H, Ching AK, Lai KP, Co NN, He M, Lung RW, Chan AW, Chan TF and Wong N: Deep sequencing of small RNA transcriptome reveals novel non-coding RNAs in hepatocellular carcinoma. J Hepatol. 58:1165–1173. 2013. View Article : Google Scholar : PubMed/NCBI | |
|
Chu H, Hui G, Yuan L, Shi D, Wang Y, Du M, Zhong D, Ma L, Tong N, Qin C, et al: Identification of novel piRNAs in bladder cancer. Cancer Lett. 356:561–567. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Li PF, Chen SC, Xia T, Jiang XM, Shao YF, Xiao BX and Guo JM: Non-coding RNAs and gastric cancer. World J Gastroenterol. 20:5411–5419. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Zhang H, Ren Y, Xu H, Pang D, Duan C and Liu C: The expression of stem cell protein Piwil2 and piR-932 in breast cancer. Surg Oncol. 22:217–223. 2013. View Article : Google Scholar : PubMed/NCBI | |
|
Janssen HL, Reesink HW, Lawitz EJ, Zeuzem S, Rodriguez-Torres M, Patel K, van der Meer AJ, Patick AK, Chen A, Zhou Y, et al: Treatment of HCV infection by targeting microRNA. N Engl J Med. 368:1685–1694. 2013. View Article : Google Scholar : PubMed/NCBI | |
|
Young DD, Connelly CM, Grohmann C and Deiters A: Small molecule modifiers of microRNA miR-122 function for the treatment of hepatitis C virus infection and hepatocellular carcinoma. J Am Chem Soc. 132:7976–7981. 2010. View Article : Google Scholar : PubMed/NCBI | |
|
Childs-Disney JL and Disney MD: Small molecule targeting of a MicroRNA associated with hepatocellular carcinoma. ACS Chem Biol. 11:375–380. 2016. View Article : Google Scholar : PubMed/NCBI | |
|
Lamb J: The Connectivity Map: A new tool for biomedical research. Nat Rev Cancer. 7:54–60. 2007. View Article : Google Scholar : PubMed/NCBI | |
|
Toyoshiba H, Sawada H, Naeshiro I and Horinouchi A: Similar compounds searching system by using the gene expression microarray database. Toxicol Lett. 186:52–57. 2009. View Article : Google Scholar : PubMed/NCBI | |
|
Kota J, Chivukula RR, O'Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR, et al: Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. Cell. 137:1005–1017. 2009. View Article : Google Scholar : PubMed/NCBI | |
|
Fang F, Chang RM, Yu L, Lei X, Xiao S, Yang H and Yang LY: MicroRNA-188-5p suppresses tumor cell proliferation and metastasis by directly targeting FGF5 in hepatocellular carcinoma. J Hepatol. 63:874–885. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Xiao Z, Ching Chow S, Han Li C, Chun Tang S, Tsui SK, Lin Z and Chen Y: Role of microRNA-95 in the anticancer activity of Brucein D in hepatocellular carcinoma. Eur J Pharmacol. 728:141–150. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Esposito T, Magliocca S, Formicola D and Gianfrancesco F: piR_015520 belongs to Piwi-associated RNAs regulates expression of the human melatonin receptor 1A gene. PLoS One. 6:e227272011. View Article : Google Scholar : PubMed/NCBI | |
|
Li X, Wu Z, Fu X and Han W: lncRNAs: Insights into their function and mechanics in underlying disorders. Mutat Res Rev Mutat Res. 762:1–21. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Bonasio R, Tu S and Reinberg D: Molecular signals of epigenetic states. Science. 330:612–616. 2010. View Article : Google Scholar : PubMed/NCBI | |
|
Delihas N: Complexity of a small non-protein coding sequence in chromosomal region 22q11.2: Presence of specialized DNA secondary structures and RNA exon/intron motifs. BMC Genomics. 16:7852015. View Article : Google Scholar : PubMed/NCBI | |
|
Jenkins AM, Waterhouse RM and Muskavitch MA: Long non-coding RNA discovery across the genus anopheles reveals conserved secondary structures within and beyond the Gambiae complex. BMC Genomics. 16:3372015. View Article : Google Scholar : PubMed/NCBI | |
|
Dhamija S and Diederichs S: From junk to master regulators of invasion: lncRNA functions in migration, EMT and metastasis. Int J Cancer. 139:269–280. 2016. View Article : Google Scholar : PubMed/NCBI | |
|
Huang B, Song JH, Cheng Y, Abraham JM, Ibrahim S, Sun Z, Ke X and Meltzer SJ: Long non-coding antisense RNA KRT7-AS is activated in gastric cancers and supports cancer cell progression by increasing KRT7 expression. Oncogene. 35:4927–4936. 2016. View Article : Google Scholar : PubMed/NCBI | |
|
Zequn N, Xuemei Z, Wei L, Zongjuan M, Yujie Z, Yanli H, Yuping Z, Xia M, Wei W, Wenjing D, et al: The role and potential mechanisms of LncRNA-TATDN1 on metastasis and invasion of non-small cell lung cancer. Oncotarget. 7:18219–18228. 2016. View Article : Google Scholar : PubMed/NCBI | |
|
Zhuang J, Lu Q, Shen B, Huang X, Shen L, Zheng X, Huang R, Yan J and Guo H: TGFβ1 secreted by cancer-associated fibroblasts induces epithelial-mesenchymal transition of bladder cancer cells through lncRNA-ZEB2NAT. Sci Rep. 5:119242015. View Article : Google Scholar : PubMed/NCBI | |
|
Liu YR, Tang RX, Huang WT, Ren FH, He RQ, Yang LH, Luo DZ, Dang YW and Chen G: Long noncoding RNAs in hepatocellular carcinoma: Novel insights into their mechanism. World J Hepatol. 7:2781–2791. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Yang X, Xie X, Xiao YF, Xie R, Hu CJ, Tang B, Li BS and Yang SM: The emergence of long non-coding RNAs in the tumorigenesis of hepatocellular carcinoma. Cancer Lett. 360:119–124. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Shibata C, Otsuka M, Kishikawa T, Ohno M, Yoshikawa T, Takata A and Koike K: Diagnostic and therapeutic application of noncoding RNAs for hepatocellular carcinoma. World J Hepatol. 7:1–6. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Huang JL, Zheng L, Hu YW and Wang Q: Characteristics of long non-coding RNA and its relation to hepatocellular carcinoma. Carcinogenesis. 35:507–514. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Tang J, Zhuo H, Zhang X, Jiang R, Ji J, Deng L, Qian X, Zhang F and Sun B: A novel biomarker Linc00974 interacting with KRT19 promotes proliferation and metastasis in hepatocellular carcinoma. Cell Death Dis. 5:e15492014. View Article : Google Scholar : PubMed/NCBI | |
|
Xu WH, Zhang JB, Dang Z, Li X, Zhou T, Liu J, Wang DS, Song WJ and Dou KF: Long non-coding RNA URHC regulates cell proliferation and apoptosis via ZAK through the ERK/MAPK signaling pathway in hepatocellular carcinoma. Int J Biol Sci. 10:664–676. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Shonkoff JP: Building a new biodevelopmental framework to guide the future of early childhood policy. Child Dev. 81:357–367. 2010. View Article : Google Scholar : PubMed/NCBI | |
|
Zheng H, Yang S, Yang Y, Yuan SX, Wu FQ, Wang LL, Yan HL, Sun SH and Zhou WP: Epigenetically silenced long noncoding-SRHC promotes proliferation of hepatocellular carcinoma. J Cancer Res Clin Oncol. 141:1195–1203. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Yu W, Qiao Y, Tang X, Ma L, Wang Y, Zhang X, Weng W, Pan Q, Yu Y, Sun F and Wang J: Tumor suppressor long non-coding RNA MT1DP is negatively regulated by YAP and Runx2 to inhibit FoxA1 in liver cancer cells. Cell Signal. 26:2961–2968. 2014. View Article : Google Scholar : PubMed/NCBI | |
|
Li C, Chen J, Zhang K, Feng B, Wang R and Chen L: Progress and prospects of long noncoding RNAs (lncRNAs) in hepatocellular carcinoma. Cell Physiol Biochem. 36:423–434. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Du Y, Kong G, You X, Zhang S, Zhang T, Gao Y, Ye L and Zhang X: Elevation of highly up-regulated in liver cancer (HULC) by hepatitis B virus X protein promotes hepatoma cell proliferation via down-regulating p18. J Biol Chem. 287:26302–26311. 2012. View Article : Google Scholar : PubMed/NCBI | |
|
Tripathi V, Ellis JD, Shen Z, Song DY, Pan Q, Watt AT, Freier SM, Bennett CF, Sharma A, Bubulya PA, et al: The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation. Mol Cell. 39:925–938. 2010. View Article : Google Scholar : PubMed/NCBI | |
|
Lin R, Roychowdhury-Saha M, Black C, Watt AT, Marcusson EG, Freier SM and Edgington TS: Control of RNA processing by a large non-coding RNA over-expressed in carcinomas. FEBS Lett. 585:671–676. 2011. View Article : Google Scholar : PubMed/NCBI | |
|
Ghosh MK, Patra F, Ghosh S, Hossain CM and Mukherjee B: Antisense oligonucleotides directed against insulin-like growth factor-II messenger ribonucleic acids delay the progress of rat hepatocarcinogenesis. J Carcinog. 13:22014. View Article : Google Scholar : PubMed/NCBI | |
|
Tedeschi L, Lande C, Cecchettini A and Citti L: Hammerhead ribozymes in therapeutic target discovery and validation. Drug Discov Today. 14:776–783. 2009. View Article : Google Scholar : PubMed/NCBI | |
|
Pavco PA, Bouhana KS, Gallegos AM, Agrawal A, Blanchard KS, Grimm SL, Jensen KL, Andrews LE, Wincott FE, Pitot PA, et al: Antitumor and antimetastatic activity of ribozymes targeting the messenger RNA of vascular endothelial growth factor receptors. Clin Cancer Res. 6:2094–2103. 2000.PubMed/NCBI | |
|
Darfeuille F, Reigadas S, Hansen JB, Orum H, Di Primo C and Toulmé JJ: Aptamers targeted to an RNA hairpin show improved specificity compared to that of complementary oligonucleotides. Biochemistry. 45:12076–12082. 2006. View Article : Google Scholar : PubMed/NCBI | |
|
Kolb G, Reigadas S, Castanotto D, Faure A, Ventura M, Rossi JJ and Toulmé JJ: Endogenous expression of an anti-TAR aptamer reduces HIV-1 replication. RNA Biol. 3:150–156. 2006. View Article : Google Scholar : PubMed/NCBI | |
|
Watrin M, Von Pelchrzim F, Dausse E, Schroeder R and Toulme JJ: In vitro selection of RNA aptamers derived from a genomic human library against the TAR RNA element of HIV-1. Biochemistry. 48:6278–6284. 2009. View Article : Google Scholar : PubMed/NCBI | |
|
Pushechnikov A, Lee MM, Childs-Disney JL, Sobczak K, French JM, Thornton CA and Disney MD: Rational design of ligands targeting triplet repeating transcripts that cause RNA dominant disease: Application to myotonic muscular dystrophy type 1 and spinocerebellar ataxia type 3. J Am Chem Soc. 131:9767–9779. 2009. View Article : Google Scholar : PubMed/NCBI | |
|
Wheeler TM, Sobczak K, Lueck JD, Osborne RJ, Lin X, Dirksen RT and Thornton CA: Reversal of RNA dominance by displacement of protein sequestered on triplet repeat RNA. Science. 325:336–339. 2009. View Article : Google Scholar : PubMed/NCBI | |
|
Parsons J, Castaldi MP, Dutta S, Dibrov SM, Wyles DL and Hermann T: Conformational inhibition of the hepatitis C virus internal ribosome entry site RNA. Nat Chem Biol. 5:823–825. 2009. View Article : Google Scholar : PubMed/NCBI | |
|
Pedram Fatemi R, Salah-Uddin S, Modarresi F, Khoury N, Wahlestedt C and Faghihi MA: Screening for small-molecule modulators of long noncoding RNA-protein interactions using alphascreen. J Biomol Screen. 20:1132–1141. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Abou-Alfa GK, Schwartz L, Ricci S, Amadori D, Santoro A, Figer A, De Greve J, Douillard JY, Lathia C, Schwartz B, et al: Phase II study of sorafenib in patients with advanced hepatocellular carcinoma. J Clin Oncol. 24:4293–4300. 2006. View Article : Google Scholar : PubMed/NCBI | |
|
Ben Mousa A: Sorafenib in the treatment of advanced hepatocellular carcinoma. Saudi J Gastroenterol. 14:40–42. 2008. View Article : Google Scholar : PubMed/NCBI | |
|
Waller LP, Deshpande V and Pyrsopoulos N: Hepatocellular carcinoma: A comprehensive review. World J Hepatol. 7:2648–2663. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
George J and Patel T: Noncoding RNA as therapeutic targets for hepatocellular carcinoma. Semin Liver Dis. 35:63–74. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Shen J, Wu WK, Ren SX, Zhang L, Chan RL, Wong CC, Lu L and Cho CH: miRNAs in gastrointestinal and liver cancers: Their perspectives and clinical applications. Curr Pharm Des. 19:1301–1310. 2013. View Article : Google Scholar : PubMed/NCBI | |
|
Movahedi F, Hu RG, Becker DL and Xu C: Stimuli-responsive liposomes for the delivery of nucleic acid therapeutics. Nanomedicine. 11:1575–1584. 2015. View Article : Google Scholar : PubMed/NCBI | |
|
Hoelder S, Clarke PA and Workman P: Discovery of small molecule cancer drugs: Successes, challenges and opportunities. Mol Oncol. 6:155–176. 2012. View Article : Google Scholar : PubMed/NCBI | |
|
Kuentz MT and Arnold Y: Influence of molecular properties on oral bioavailability of lipophilic drugs-mapping of bulkiness and different measures of polarity. Pharm Dev Technol. 14:312–320. 2009. View Article : Google Scholar : PubMed/NCBI |
