Suv4‑20h1 promotes G1 to S phase transition by downregulating p21WAF1/CIP1 expression in chronic myeloid leukemia K562 cells

Wu,Yupeng; Wang,Yadong; Liu,Ming; Nie,Min; Wang,Ying; Deng,Yexuan; Yao,Bing; Gui,Tao; Li,Xinyu; Ma,Lingling; Guo,Chan; Ma,Chi; Ju,Junyi; Zhao,Quan

doi:10.3892/ol.2018.8092

Suv4‑20h1 promotes G1 to S phase transition by downregulating p21WAF1/CIP1 expression in chronic myeloid leukemia K562 cells

Authors:
- Yupeng Wu
- Yadong Wang
- Ming Liu
- Min Nie
- Ying Wang
- Yexuan Deng
- Bing Yao
- Tao Gui
- Xinyu Li
- Lingling Ma
- Chan Guo
- Chi Ma
- Junyi Ju
- Quan Zhao
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Affiliations: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, P.R. China
Published online on: February 21, 2018 https://doi.org/10.3892/ol.2018.8092
Pages: 6123-6130
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Methylation of histone H4 lysine 20 (H4K20) has been associated with cancer. However, the functions of the histone methyltransferases that trigger histone H4K20 methylation in cancers, including suppressor of variegation 4‑20 homolog 1 (Suv4‑20h1), remain elusive. In the present study, it was demonstrated that the knockdown of the histone H4K20 methyltransferase Suv4‑20h1 resulted in growth inhibition in chronic myeloid leukemia K562 cells. Disruption of Suv4‑20h1 expression induced G1 arrest in the cell cycle and increased expression levels of cyclin dependent kinase inhibitor 1A (p21WAF1/CIP1), an essential cell cycle protein involved in checkpoint regulation. Chromatin immunoprecipitation analysis demonstrated that Suv4‑20h1 directly binds to the promoter of the p21 gene and that methylation of histone H4K20 correlates with repression of p21 expression. Thus, these data suggest that Suv4‑20h1 is important for the regulation of the cell cycle in K562 cells and may be a potential therapeutic target for leukemia.

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