miR-130b-5p promotes proliferation, migration and invasion of gastric cancer cells via targeting RASAL1
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- Published online on: March 5, 2018 https://doi.org/10.3892/ol.2018.8174
- Pages: 6361-6367
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Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
The aim of the present study was to investigate the targeted interaction between microRNA (miR)‑130b‑5p and RAS protein activator like 1 (RASAL1) gene and elucidate the function of miR‑130b‑5p in cell proliferation, migration and invasion in gastric cancer. Expression of miR‑130b‑5p and RASAL1 in seven gastric cell lines was detected by reverse transcription-quantitative polymerase chain reaction (RT‑qPCR). MGC803 cells were selected for further study since they exhibited a marked increase in expression of miR‑130b‑5p accompanied by decreased expression of RASAL1. MGC803 cells were transfected with miR‑130b‑5p mimics and miR‑130b‑5p inhibitor using Lipofectamine 2000 for over‑ and underexpression, respectively, with cells transfected with negative control (NC) sequence as the control. In addition, a luciferase reporter gene assay was performed to evaluate the targeted interaction between miR‑130b‑5p and RASAL1. Then, alterations in RASAL1 expression were detected by RT‑qPCR and western blot analysis following transfection with miR‑130b‑5p mimics and miR‑130b‑5p inhibitor. Cell proliferation, colony formation, and migration and invasion ability were detected by MTT, colony formation and Transwell assays, respectively. RASAL1 was demonstrated to be a target gene of miR‑130b‑5p by luciferase reporter gene assay. In addition, the expression of RASAL1 was significantly lower in MGC803 cells that were transfected with miR‑130b‑5p mimics and significantly higher in cells transfected with miR‑130b‑5p inhibitor in comparison with cells transfected with NC (P<0.05). Furthermore, the experimental group transfected with miR‑130b‑5p mimics manifested significantly higher cell proliferation, increased colony formation and increased migratory and invasive capacities (P<0.05). By contrast, cells transfected with miR‑130b‑5p inhibitor exhibited significantly lower cell proliferation, decreased colony formation and decreased migratory and invasive capacities, compared with cells transfected with NC (P<0.05). In conclusion, RASAL1 was demonstrated to be a target gene of miR‑130b‑5p. Overexpression of miR‑130b‑5p results in promoted proliferation, colony formation and migration and invasion abilities through targeted modulation of RASAL1.
