The correlation between TNF-α-308 gene polymorphism and susceptibility to cervical cancer

Li,Liping; Liu,Jie; Liu,Chunjing; Lu,Xianghui

doi:10.3892/ol.2018.8246

The correlation between TNF-α-308 gene polymorphism and susceptibility to cervical cancer

Authors:
- Liping Li
- Jie Liu
- Chunjing Liu
- Xianghui Lu
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Affiliations: Department of Gynaecology and Obstetrics, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161000, P.R. China
Published online on: March 12, 2018 https://doi.org/10.3892/ol.2018.8246
Pages: 7163-7167
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tumor necrosis factor-α (TNF-α) is closely related to the occurrence of human cancers. Cervical cancer seriously affects female health. Therefore, our study aimed to investigate the correlation between the polymorphism of TNF‑α‑308 gene and susceptibility to cervical cancer. Whole blood was collected from 142 patients with cervical cancer and 150 healthy controls. PCR‑RFLP was used to detect the polymorphism of TNF‑α‑308 and the correlation between polymorphism of TNF‑α‑308 and the susceptibility to cervical cancer was analyzed. The three genotypes of TNF‑α‑308 were GG, GA and AA, and the distributions of genotypes of TNF‑α‑308 were consistent with Hardy‑Weinberg equilibrium in both cervical cancer group and control group. There were no significant differences in genotype and allele frequency between cervical cancer group and healthy control group (P>0.05). A/A genotype increased the risk of cervical cancer by 1.46 times with 95% confidence interval of 0.32-6.67. Different genotypes were not associated with tumor type (P>0.05). Different genotypes are correlated with cervical cancer TNM stages, tumor differentiation and lymph node metastasis. Proportion of GA+AA genotype in TNM stage III+IV group, low differentiation group and lymph node metastasis group were 28.1, 29.0 and 29.8%, respectively, which were significantly higher than those in stage I+II group, moderate/high differentiation group and non‑lymph node metastasis group (P<0.05). The results suggested that TNF‑α‑308 gene polymorphism is associated with the degree of malignancy of cervical cancer. Female patients with A allele have higher malignant degree of cervical cancer.

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