Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells

Yang,Hai‑Bo; Gao,Hui‑Ru; Ren,Yuan‑Jing; Fang,Fei‑Xiang; Tian,Hong‑Tao; Gao,Zhen‑Jiang; Song,Wei; Huang,Shao‑Min; Zhao,An‑Fang

doi:10.3892/ol.2018.8303

Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells

Authors:
- Hai‑Bo Yang
- Hui‑Ru Gao
- Yuan‑Jing Ren
- Fei‑Xiang Fang
- Hong‑Tao Tian
- Zhen‑Jiang Gao
- Wei Song
- Shao‑Min Huang
- An‑Fang Zhao
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Affiliations: Department of Biological Pharmacy, School of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan 467044, P.R. China, Department of Obstetrics and Gynecology, Center for Reproductive Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361004, P.R. China
Published online on: March 20, 2018 https://doi.org/10.3892/ol.2018.8303
Pages: 7993-7998

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Abstract

Resistance to apoptosis is an characteristic of cancer cells that serves a critical function in tumor development and represents a target for antitumor therapy. Isoimperatorin (ISOIM), a coumarin compound, exhibits antitumor functions in multiple types of tumor cells. However, its antitumor effects and molecular mechanisms with respect to gastric cancer have not been elucidated. The present study assessed the anti‑proliferative and apoptotic effects of ISOIM on human BGC‑823 gastric cancer cells and elucidated its underlying molecular mechanisms. Cell proliferation was evaluated using MTT assays. Analysis of cell morphology was performed by hematoxylin and eosin, Hoechst 33258 and acridine orange/ethidium bromide staining. In addition, cell cycle and apoptosis was evaluated using flow cytometry analysis; expression of apoptosis‑associated proteins was studied by western blotting. The results of the present study revealed that ISOIM signiﬁcantly inhibited cell proliferation by arresting the cell cycle at the G2/M phase and induced apoptosis by increasing Bcl‑2‑associated X (Bax) expression with a concomitant decrease in Bcl‑2 expression, resulting in a decreased Bcl‑2/Bax ratio compared with the control. In addition, ISOIM treatment also resulted in cytochrome c translocating from the mitochondria to the cytosol. Furthermore, caspase‑3 was significantly activated in response to treatment with ISOIM, suggesting that apoptosis in BGC‑823 cells is induced in the mitochondrial pathway. Taken together, the results of the present study indicate that ISOIM may signiﬁcantly induce apoptosis in BGC‑823 cells and that the pro‑apoptotic mechanisms of ISOIM could be associated with the mitochondrial pathway.

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