miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways

  • Authors:
    • Xiaofeng Han
    • Jianwei Zheng
    • Yunlei Wang
    • Zhigang Gao
  • View Affiliations

  • Published online on: June 5, 2018     https://doi.org/10.3892/ol.2018.8905
  • Pages: 2638-2644
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Abstract

In the present study, the effects of microRNA‑29a (miRNA‑29a) on colon cancer cell viability and the molecular mechanisms underlying the effects were investigated. The expression of miRNA‑29a in colon cancer serum samples was notably downregulated, compared with in the normal group. First, miRNA‑29a mimic was used to increase the expression of miRNA‑29a in HCT‑116 cells. Furthermore, upregulation of miRNA‑29a suppressed cell viability, increased lactate dehydrogenase levels and apoptosis, and promoted caspase‑3/9 activities and B‑cell lymphoma 2‑associated X protein and phosphatase and tensin homolog (PTEN) protein expression in colon cancer cells. Furthermore, upregulation of miRNA‑29a decreased phosphoinositide 3‑kinase, phosphorylated (p)‑protein kinase B (Akt) and p‑glycogen synthase kinase 3β (GSK3β) protein expression and suppressed the Wnt/β‑catenin signaling pathway in colon cancer cells. The results of the present study verified that the protective effects of miRNA‑29a suppress the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways in colon cancer.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Han X, Zheng J, Wang Y and Gao Z: miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways. Oncol Lett 16: 2638-2644, 2018.
APA
Han, X., Zheng, J., Wang, Y., & Gao, Z. (2018). miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways. Oncology Letters, 16, 2638-2644. https://doi.org/10.3892/ol.2018.8905
MLA
Han, X., Zheng, J., Wang, Y., Gao, Z."miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways". Oncology Letters 16.2 (2018): 2638-2644.
Chicago
Han, X., Zheng, J., Wang, Y., Gao, Z."miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways". Oncology Letters 16, no. 2 (2018): 2638-2644. https://doi.org/10.3892/ol.2018.8905