Microbiota dysbiosis is associated with HPV‑induced cervical carcinogenesis

Kwasniewski,Wojciech; Wolun‑Cholewa,Maria; Kotarski,Jan; Warchol,Wojciech; Kuzma,Dorota; Kwasniewska,Anna; Gozdzicka‑Jozefiak,Anna

doi:10.3892/ol.2018.9509

Microbiota dysbiosis is associated with HPV‑induced cervical carcinogenesis

Authors:
- Wojciech Kwasniewski
- Maria Wolun‑Cholewa
- Jan Kotarski
- Wojciech Warchol
- Dorota Kuzma
- Anna Kwasniewska
- Anna Gozdzicka‑Jozefiak
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Affiliations: Department of Gynaecological Oncology and Gynaecology, Medical University of Lublin, 20‑081 Lublin, Poland, Department of Cell Biology, Poznan University of Medical Sciences, 61‑701 Poznan, Poland, Department of Biophysics, Poznan University of Medical Sciences, 61‑701 Poznan, Poland, Department of Molecular Virology, Adam Mickiewicz University, 61‑712 Poznan, Poland, Department of Obstetrics and Gynaecology, Medical University of Lublin, 20‑081 Lublin, Poland
Published online on: September 26, 2018 https://doi.org/10.3892/ol.2018.9509
Pages: 7035-7047

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Abstract

Cervical microbial communities serve a crucial role in the persistence and development of oncogenic human papilloma virus (HPV) infections. In the present study, the authors hypothesised that disturbed heterogeneity of microbial flora was associated with HPV‑induced carcinogenesis. Swabs of the cervical microbiota were collected from 250 women and the 16S ribosomal DNA was sequenced using a high throughput assay. The swabs of cervical microbiota were grouped according to the community state types (CSTs) as follows: Healthy cervical swabs; swabs taken from low‑grade squamous intra‑epithelial lesions (LSIL) and swabs taken from high‑grade squamous intra‑epithelial lesions (HSIL). Analysis of the bacterial classes revealed that the CST cervical swabs of the volunteers were characterised by Lactobacillus crispatus, Lactobacillus iners and Lactobacillus taiwanensis, however, Gardnerella vaginalis and Lactobacillus acidophilus were absent. In the CST of patients with LSIL the predominant type of bacteria was Lactobacillus acidophilus and Lactobacillus iners, however Lactobacillus crispatus was not detected. Swabs from CST women diagnosed with HSIL exhibited abundant Gardnerella vaginalis and Lactobacillus acidophilus, however, lacked Lactobacillus taiwanensis, Lactobacillus iners and Lactobacillus crispatus. The abundance of Lactobacillus acidophilus in swabs from the healthy women was compared with the swabs from the women with LSIL. The results of the present study indicated that the development of HPV‑induced cancer is associated with a high diversity of vaginal microbiota, which is involved in the control of viral persistence, and is therefore indicative of disease prognosis.

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